Consistent acidogenic co-fermentation of waste activated sludge and food waste under thermophilic conditions

Acidogenic co-fermentation of waste activated sludge (WAS) and food waste (FW) under thermophilic conditions enhances process consistency, while overcoming the problem of acetic acid consumption due to growing methanogens. Two long-term continuous co-fermentation experiments were carried out with a...

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Autores: Pérez Esteban, Noemí, Tully, Rose, Peces, Miriam, Dosta Parras, Joan, Astals Garcia, Sergi
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/224655
Acceso en línea:https://hdl.handle.net/2445/224655
Access Level:acceso abierto
Palabra clave:Metabolisme microbià
Microbial metabolism
Anaerobic digestion
Thermophilic microorganisms
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spelling Consistent acidogenic co-fermentation of waste activated sludge and food waste under thermophilic conditionsPérez Esteban, NoemíTully, RosePeces, MiriamDosta Parras, JoanAstals Garcia, SergiMetabolisme microbiàMicrobial metabolismAnaerobic digestionThermophilic microorganismsAcidogenic co-fermentation of waste activated sludge (WAS) and food waste (FW) under thermophilic conditions enhances process consistency, while overcoming the problem of acetic acid consumption due to growing methanogens. Two long-term continuous co-fermentation experiments were carried out with a WAS:FW mixture (70:30 % in VS) at organic loading rate of 8 gVS/(L·d). Experiment 1 assessed the impact of temperature (35 °C and 55 °C) and WAS origin (WAS_A and WAS_B) in two collection periods. Experiment 2 evaluated the consistency at 55 °C by testing three WAS origins (WAS_A, WAS_B and WAS_C) in 3 additional collection periods. Experimental results showed that at 55 °C, the solubilisation yield was enhanced compared to 35 °C, although this did not always lead to higher fermentation yield. The fermentation product profile was affected by the operating temperature, with 55 °C promoting the accumulation of acetic and butyric acids. Acetic acid consumption was only detected at 35 °C in fermenters treating WAS_A, whereas it was not observed in fermenters treating WAS_B. This consumption was prevented at 55 °C, as none of the 13 fermenters continuous operation showed acetic acid consumption. Acetic acid consumption was attributed to species midas_s_9557 (genus Methanosarcina), an aceticlastic methanogen, which did not grow under 55 °C. Temperature had a more significant effect on the microbial community structure than WAS origin. Functional redundancy was demonstrated by each fermenter having its own distinct microbial consortium while maintaining constant metabolic functions at 55 °C. Overall, the acidogenic co-fermentation of WAS and FW at 55 °C is regarded as a robust and consistent biotechnology.Elsevier Ltd.2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/224655Articles publicats en revistes (Enginyeria Química i Química Analítica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1016/j.watres.2024.122970Water Research, 2025, vol. 271https://doi.org/10.1016/j.watres.2024.122970cc-by-nc-nd (c) Pérez Esteban, Noemí et al., 2025http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2246552026-05-27T06:46:51Z
dc.title.none.fl_str_mv Consistent acidogenic co-fermentation of waste activated sludge and food waste under thermophilic conditions
title Consistent acidogenic co-fermentation of waste activated sludge and food waste under thermophilic conditions
spellingShingle Consistent acidogenic co-fermentation of waste activated sludge and food waste under thermophilic conditions
Pérez Esteban, Noemí
Metabolisme microbià
Microbial metabolism
Anaerobic digestion
Thermophilic microorganisms
title_short Consistent acidogenic co-fermentation of waste activated sludge and food waste under thermophilic conditions
title_full Consistent acidogenic co-fermentation of waste activated sludge and food waste under thermophilic conditions
title_fullStr Consistent acidogenic co-fermentation of waste activated sludge and food waste under thermophilic conditions
title_full_unstemmed Consistent acidogenic co-fermentation of waste activated sludge and food waste under thermophilic conditions
title_sort Consistent acidogenic co-fermentation of waste activated sludge and food waste under thermophilic conditions
dc.creator.none.fl_str_mv Pérez Esteban, Noemí
Tully, Rose
Peces, Miriam
Dosta Parras, Joan
Astals Garcia, Sergi
author Pérez Esteban, Noemí
author_facet Pérez Esteban, Noemí
Tully, Rose
Peces, Miriam
Dosta Parras, Joan
Astals Garcia, Sergi
author_role author
author2 Tully, Rose
Peces, Miriam
Dosta Parras, Joan
Astals Garcia, Sergi
author2_role author
author
author
author
dc.subject.none.fl_str_mv Metabolisme microbià
Microbial metabolism
Anaerobic digestion
Thermophilic microorganisms
topic Metabolisme microbià
Microbial metabolism
Anaerobic digestion
Thermophilic microorganisms
description Acidogenic co-fermentation of waste activated sludge (WAS) and food waste (FW) under thermophilic conditions enhances process consistency, while overcoming the problem of acetic acid consumption due to growing methanogens. Two long-term continuous co-fermentation experiments were carried out with a WAS:FW mixture (70:30 % in VS) at organic loading rate of 8 gVS/(L·d). Experiment 1 assessed the impact of temperature (35 °C and 55 °C) and WAS origin (WAS_A and WAS_B) in two collection periods. Experiment 2 evaluated the consistency at 55 °C by testing three WAS origins (WAS_A, WAS_B and WAS_C) in 3 additional collection periods. Experimental results showed that at 55 °C, the solubilisation yield was enhanced compared to 35 °C, although this did not always lead to higher fermentation yield. The fermentation product profile was affected by the operating temperature, with 55 °C promoting the accumulation of acetic and butyric acids. Acetic acid consumption was only detected at 35 °C in fermenters treating WAS_A, whereas it was not observed in fermenters treating WAS_B. This consumption was prevented at 55 °C, as none of the 13 fermenters continuous operation showed acetic acid consumption. Acetic acid consumption was attributed to species midas_s_9557 (genus Methanosarcina), an aceticlastic methanogen, which did not grow under 55 °C. Temperature had a more significant effect on the microbial community structure than WAS origin. Functional redundancy was demonstrated by each fermenter having its own distinct microbial consortium while maintaining constant metabolic functions at 55 °C. Overall, the acidogenic co-fermentation of WAS and FW at 55 °C is regarded as a robust and consistent biotechnology.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/224655
url https://hdl.handle.net/2445/224655
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.watres.2024.122970
Water Research, 2025, vol. 271
https://doi.org/10.1016/j.watres.2024.122970
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Pérez Esteban, Noemí et al., 2025
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Pérez Esteban, Noemí et al., 2025
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier Ltd.
publisher.none.fl_str_mv Elsevier Ltd.
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Química i Química Analítica)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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