Characterisation of the resistome and virulome of Pseudomonas aeruginosa isolates from patients with ventilatorassociated pneumonia

ABSTRACT : Introduction/objective: Ventilator-associated pneumonia (VAP) is the hospital-acquired infection with the greatest impact on patient outcomes and health care costs. Endogenous colonization by aerobic gram-negative bacteria such as Pseudomonas aeruginosa play a pivotal role in the pathogen...

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Autor: Viadero Valderrama, Carlos
Tipo de recurso: tesis de maestría
Fecha de publicación:2020
País:España
Institución:Universidad de Cantabria (UC)
Repositorio:UCrea Repositorio Abierto de la Universidad de Cantabria
Idioma:inglés
OAI Identifier:oai:repositorio.unican.es:10902/20407
Acceso en línea:http://hdl.handle.net/10902/20407
Access Level:acceso abierto
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spelling Characterisation of the resistome and virulome of Pseudomonas aeruginosa isolates from patients with ventilatorassociated pneumoniaViadero Valderrama, CarlosABSTRACT : Introduction/objective: Ventilator-associated pneumonia (VAP) is the hospital-acquired infection with the greatest impact on patient outcomes and health care costs. Endogenous colonization by aerobic gram-negative bacteria such as Pseudomonas aeruginosa play a pivotal role in the pathogenesis of VAP. The aim of this work was the characterisation of the resistome and virulome of a collection of P. aeruginosa isolates from patients with VAP collected during a multicentre study. Material and methods: We analysed 38 isolates (1 per patient) of P. aeruginosa from patients with VAP. Minimum inhibitory concentrations (MICs) of 16 different antibiotics were determined by broth microdilution according to the Clinical and Laboratory Standard Institute (CLSI) and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) clinical breakpoints. The complete genomes of all the isolates were sequenced using the MiSeq platform (Illumina) to determine their clonal relationship, as well as the presence of antibiotic resistance and virulence related genes. Results: According to antibiotic resistance, 10.5% (n=4) of the isolates were multi-susceptible and 23.7% (n=9) moderately resistant, while 21.1% (n=8) were multidrug-resistant and 44.7% (n=17) were extensively resistant. Forty-five genes related with antibiotic resistance were detected: blaGES-7 and blaVIM (1,2 and 20) were observed on extensively resistant isolates. According to the results obtained from the Virulence finder database (VFDB), the virulome of the whole population was made up of 294 genes, among which the most significant were the effectors (exo-) from the type III secretion system.Máster en Biología Molecular y BiomedicinaOcampo Sosa, Alain AntonioUniversidad de Cantabria20202020-10-15master thesishttp://purl.org/coar/resource_type/c_bdccNAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/masterThesishttp://hdl.handle.net/10902/20407reponame:UCrea Repositorio Abierto de la Universidad de Cantabriainstname:Universidad de Cantabria (UC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:repositorio.unican.es:10902/204072026-06-02T12:39:31Z
dc.title.none.fl_str_mv Characterisation of the resistome and virulome of Pseudomonas aeruginosa isolates from patients with ventilatorassociated pneumonia
title Characterisation of the resistome and virulome of Pseudomonas aeruginosa isolates from patients with ventilatorassociated pneumonia
spellingShingle Characterisation of the resistome and virulome of Pseudomonas aeruginosa isolates from patients with ventilatorassociated pneumonia
Viadero Valderrama, Carlos
title_short Characterisation of the resistome and virulome of Pseudomonas aeruginosa isolates from patients with ventilatorassociated pneumonia
title_full Characterisation of the resistome and virulome of Pseudomonas aeruginosa isolates from patients with ventilatorassociated pneumonia
title_fullStr Characterisation of the resistome and virulome of Pseudomonas aeruginosa isolates from patients with ventilatorassociated pneumonia
title_full_unstemmed Characterisation of the resistome and virulome of Pseudomonas aeruginosa isolates from patients with ventilatorassociated pneumonia
title_sort Characterisation of the resistome and virulome of Pseudomonas aeruginosa isolates from patients with ventilatorassociated pneumonia
dc.creator.none.fl_str_mv Viadero Valderrama, Carlos
author Viadero Valderrama, Carlos
author_facet Viadero Valderrama, Carlos
author_role author
dc.contributor.none.fl_str_mv Ocampo Sosa, Alain Antonio
Universidad de Cantabria
description ABSTRACT : Introduction/objective: Ventilator-associated pneumonia (VAP) is the hospital-acquired infection with the greatest impact on patient outcomes and health care costs. Endogenous colonization by aerobic gram-negative bacteria such as Pseudomonas aeruginosa play a pivotal role in the pathogenesis of VAP. The aim of this work was the characterisation of the resistome and virulome of a collection of P. aeruginosa isolates from patients with VAP collected during a multicentre study. Material and methods: We analysed 38 isolates (1 per patient) of P. aeruginosa from patients with VAP. Minimum inhibitory concentrations (MICs) of 16 different antibiotics were determined by broth microdilution according to the Clinical and Laboratory Standard Institute (CLSI) and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) clinical breakpoints. The complete genomes of all the isolates were sequenced using the MiSeq platform (Illumina) to determine their clonal relationship, as well as the presence of antibiotic resistance and virulence related genes. Results: According to antibiotic resistance, 10.5% (n=4) of the isolates were multi-susceptible and 23.7% (n=9) moderately resistant, while 21.1% (n=8) were multidrug-resistant and 44.7% (n=17) were extensively resistant. Forty-five genes related with antibiotic resistance were detected: blaGES-7 and blaVIM (1,2 and 20) were observed on extensively resistant isolates. According to the results obtained from the Virulence finder database (VFDB), the virulome of the whole population was made up of 294 genes, among which the most significant were the effectors (exo-) from the type III secretion system.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-10-15
dc.type.none.fl_str_mv master thesis
http://purl.org/coar/resource_type/c_bdcc
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
dc.identifier.none.fl_str_mv http://hdl.handle.net/10902/20407
url http://hdl.handle.net/10902/20407
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:UCrea Repositorio Abierto de la Universidad de Cantabria
instname:Universidad de Cantabria (UC)
instname_str Universidad de Cantabria (UC)
reponame_str UCrea Repositorio Abierto de la Universidad de Cantabria
collection UCrea Repositorio Abierto de la Universidad de Cantabria
repository.name.fl_str_mv
repository.mail.fl_str_mv
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