Intranasal Administration of Undifferentiated Oligodendrocyte Lineage Cells as a Potential Approach to Deliver Oligodendrocyte Precursor Cells into Brain

Oligodendrocyte precursor cell (OPC) migration is a mechanism involved in remyelination; these cells migrate from niches in the adult CNS. However, age and disease reduce the pool of OPCs; as a result, the remyelination capacity of the CNS decreases over time. Several experimental studies have intro...

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Autores: Gómez Pinedo, Ulises, Matias Guiu, Jordi A., Benito Martín, María Soledad, Moreno Jiménez, Lidia, Sanclemente Alamán, Inmaculada, Selma Calvo, Belen, Pérez Suárez:, Sara, Sancho Bielsa, Francisco, Canales Aguirre, Alejandro, Mateos Díaz, Juan Carlos, Hernández Sapiéns, Mercedes A., Reza Zaldívar, Edwin E., Ojeda Hernández, Doddy Denise, Vidorreta Ballesteros, Lucía, Montero Escribano, Paloma, Matías-Guiu Guía, Jorge
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/4838
Acceso en línea:https://hdl.handle.net/20.500.14352/4838
Access Level:acceso abierto
Palabra clave:Multiple sclerosis
HOG cells
Oligodendrocyte precursor cells
Oligodendrocytes
Intranasal administration
Demyelination
Remyelination
Farmacología (Medicina)
Neurociencias (Medicina)
2490 Neurociencias
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spelling Intranasal Administration of Undifferentiated Oligodendrocyte Lineage Cells as a Potential Approach to Deliver Oligodendrocyte Precursor Cells into BrainGómez Pinedo, UlisesMatias Guiu, Jordi A.Benito Martín, María SoledadMoreno Jiménez, LidiaSanclemente Alamán, InmaculadaSelma Calvo, BelenPérez Suárez:, SaraSancho Bielsa, FranciscoCanales Aguirre, AlejandroMateos Díaz, Juan CarlosHernández Sapiéns, Mercedes A.Reza Zaldívar, Edwin E.Ojeda Hernández, Doddy DeniseVidorreta Ballesteros, LucíaMontero Escribano, PalomaMatías-Guiu Guía, JorgeMultiple sclerosisHOG cellsOligodendrocyte precursor cellsOligodendrocytesIntranasal administrationDemyelinationRemyelinationFarmacología (Medicina)Neurociencias (Medicina)2490 NeurocienciasOligodendrocyte precursor cell (OPC) migration is a mechanism involved in remyelination; these cells migrate from niches in the adult CNS. However, age and disease reduce the pool of OPCs; as a result, the remyelination capacity of the CNS decreases over time. Several experimental studies have introduced OPCs to the brain via direct injection or intrathecal administration. In this study, we used the nose-to brain pathway to deliver oligodendrocyte lineage cells (human oligodendroglioma (HOG) cells), which behave similarly to OPCs in vitro. To this end, we administered GFP-labelled HOG cells intranasally to experimental animals, which were subsequently euthanised at 30 or 60 days. Our results show that the intranasal route is a viable route to the CNS and that HOG cells administered intranasally migrate preferentially to niches of OPCs (clusters created during embryonic development and adult life). Our study provides evidence, albeit limited, that HOG cells either form clusters or adhere to clusters of OPCs in the brains of experimental animals.MPDIUniversidad Complutense de Madrid20212021-10-0420212021-10-04journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/4838reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/48382026-06-02T12:44:21Z
dc.title.none.fl_str_mv Intranasal Administration of Undifferentiated Oligodendrocyte Lineage Cells as a Potential Approach to Deliver Oligodendrocyte Precursor Cells into Brain
title Intranasal Administration of Undifferentiated Oligodendrocyte Lineage Cells as a Potential Approach to Deliver Oligodendrocyte Precursor Cells into Brain
spellingShingle Intranasal Administration of Undifferentiated Oligodendrocyte Lineage Cells as a Potential Approach to Deliver Oligodendrocyte Precursor Cells into Brain
Gómez Pinedo, Ulises
Multiple sclerosis
HOG cells
Oligodendrocyte precursor cells
Oligodendrocytes
Intranasal administration
Demyelination
Remyelination
Farmacología (Medicina)
Neurociencias (Medicina)
2490 Neurociencias
title_short Intranasal Administration of Undifferentiated Oligodendrocyte Lineage Cells as a Potential Approach to Deliver Oligodendrocyte Precursor Cells into Brain
title_full Intranasal Administration of Undifferentiated Oligodendrocyte Lineage Cells as a Potential Approach to Deliver Oligodendrocyte Precursor Cells into Brain
title_fullStr Intranasal Administration of Undifferentiated Oligodendrocyte Lineage Cells as a Potential Approach to Deliver Oligodendrocyte Precursor Cells into Brain
title_full_unstemmed Intranasal Administration of Undifferentiated Oligodendrocyte Lineage Cells as a Potential Approach to Deliver Oligodendrocyte Precursor Cells into Brain
title_sort Intranasal Administration of Undifferentiated Oligodendrocyte Lineage Cells as a Potential Approach to Deliver Oligodendrocyte Precursor Cells into Brain
dc.creator.none.fl_str_mv Gómez Pinedo, Ulises
Matias Guiu, Jordi A.
Benito Martín, María Soledad
Moreno Jiménez, Lidia
Sanclemente Alamán, Inmaculada
Selma Calvo, Belen
Pérez Suárez:, Sara
Sancho Bielsa, Francisco
Canales Aguirre, Alejandro
Mateos Díaz, Juan Carlos
Hernández Sapiéns, Mercedes A.
Reza Zaldívar, Edwin E.
Ojeda Hernández, Doddy Denise
Vidorreta Ballesteros, Lucía
Montero Escribano, Paloma
Matías-Guiu Guía, Jorge
author Gómez Pinedo, Ulises
author_facet Gómez Pinedo, Ulises
Matias Guiu, Jordi A.
Benito Martín, María Soledad
Moreno Jiménez, Lidia
Sanclemente Alamán, Inmaculada
Selma Calvo, Belen
Pérez Suárez:, Sara
Sancho Bielsa, Francisco
Canales Aguirre, Alejandro
Mateos Díaz, Juan Carlos
Hernández Sapiéns, Mercedes A.
Reza Zaldívar, Edwin E.
Ojeda Hernández, Doddy Denise
Vidorreta Ballesteros, Lucía
Montero Escribano, Paloma
Matías-Guiu Guía, Jorge
author_role author
author2 Matias Guiu, Jordi A.
Benito Martín, María Soledad
Moreno Jiménez, Lidia
Sanclemente Alamán, Inmaculada
Selma Calvo, Belen
Pérez Suárez:, Sara
Sancho Bielsa, Francisco
Canales Aguirre, Alejandro
Mateos Díaz, Juan Carlos
Hernández Sapiéns, Mercedes A.
Reza Zaldívar, Edwin E.
Ojeda Hernández, Doddy Denise
Vidorreta Ballesteros, Lucía
Montero Escribano, Paloma
Matías-Guiu Guía, Jorge
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv Multiple sclerosis
HOG cells
Oligodendrocyte precursor cells
Oligodendrocytes
Intranasal administration
Demyelination
Remyelination
Farmacología (Medicina)
Neurociencias (Medicina)
2490 Neurociencias
topic Multiple sclerosis
HOG cells
Oligodendrocyte precursor cells
Oligodendrocytes
Intranasal administration
Demyelination
Remyelination
Farmacología (Medicina)
Neurociencias (Medicina)
2490 Neurociencias
description Oligodendrocyte precursor cell (OPC) migration is a mechanism involved in remyelination; these cells migrate from niches in the adult CNS. However, age and disease reduce the pool of OPCs; as a result, the remyelination capacity of the CNS decreases over time. Several experimental studies have introduced OPCs to the brain via direct injection or intrathecal administration. In this study, we used the nose-to brain pathway to deliver oligodendrocyte lineage cells (human oligodendroglioma (HOG) cells), which behave similarly to OPCs in vitro. To this end, we administered GFP-labelled HOG cells intranasally to experimental animals, which were subsequently euthanised at 30 or 60 days. Our results show that the intranasal route is a viable route to the CNS and that HOG cells administered intranasally migrate preferentially to niches of OPCs (clusters created during embryonic development and adult life). Our study provides evidence, albeit limited, that HOG cells either form clusters or adhere to clusters of OPCs in the brains of experimental animals.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-10-04
2021
2021-10-04
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/4838
url https://hdl.handle.net/20.500.14352/4838
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MPDI
publisher.none.fl_str_mv MPDI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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