Intranasal Administration of Undifferentiated Oligodendrocyte Lineage Cells as a Potential Approach to Deliver Oligodendrocyte Precursor Cells into Brain

Oligodendrocyte precursor cell (OPC) migration is a mechanism involved in remyelination; these cells migrate from niches in the adult CNS. However, age and disease reduce the pool of OPCs; as a result, the remyelination capacity of the CNS decreases over time. Several experimental studies have intro...

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Detalles Bibliográficos
Autores: Gómez Pinedo, Ulises, Matias Guiu, Jordi A., Benito Martín, María Soledad, Moreno Jiménez, Lidia, Sanclemente Alamán, Inmaculada, Selma Calvo, Belen, Pérez Suárez:, Sara, Sancho Bielsa, Francisco, Canales Aguirre, Alejandro, Mateos Díaz, Juan Carlos, Hernández Sapiéns, Mercedes A., Reza Zaldívar, Edwin E., Ojeda Hernández, Doddy Denise, Vidorreta Ballesteros, Lucía, Montero Escribano, Paloma, Matías-Guiu Guía, Jorge
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/4838
Acceso en línea:https://hdl.handle.net/20.500.14352/4838
Access Level:acceso abierto
Palabra clave:Multiple sclerosis
HOG cells
Oligodendrocyte precursor cells
Oligodendrocytes
Intranasal administration
Demyelination
Remyelination
Farmacología (Medicina)
Neurociencias (Medicina)
2490 Neurociencias
Descripción
Sumario:Oligodendrocyte precursor cell (OPC) migration is a mechanism involved in remyelination; these cells migrate from niches in the adult CNS. However, age and disease reduce the pool of OPCs; as a result, the remyelination capacity of the CNS decreases over time. Several experimental studies have introduced OPCs to the brain via direct injection or intrathecal administration. In this study, we used the nose-to brain pathway to deliver oligodendrocyte lineage cells (human oligodendroglioma (HOG) cells), which behave similarly to OPCs in vitro. To this end, we administered GFP-labelled HOG cells intranasally to experimental animals, which were subsequently euthanised at 30 or 60 days. Our results show that the intranasal route is a viable route to the CNS and that HOG cells administered intranasally migrate preferentially to niches of OPCs (clusters created during embryonic development and adult life). Our study provides evidence, albeit limited, that HOG cells either form clusters or adhere to clusters of OPCs in the brains of experimental animals.

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