Low field nuclear magnetic resonance (LF-NMR) relaxometry in hake (Merluccius merluccius, L.) muscle after different freezing and storage conditions

Low field NMR T2 transverse relaxation measurements were performed on muscle samples from sixty hake (Merluccius merluccius, L.). Fish fillets from hake stored in ice for 3 and 14 days were subjected to different freezing methods (air blast, liquid nitrogen or walk-in freezer) and storage conditions...

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Detalles Bibliográficos
Autores: Sánchez Alonso, Isabel, Moreno, Pilar, Careche, Mercedes
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/93006
Acceso en línea:http://hdl.handle.net/10261/93006
Access Level:acceso abierto
Palabra clave:Freezing
Frozen storage
Low field NMR
Ice crystal
Quality
Fish
Muscle
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spelling Low field nuclear magnetic resonance (LF-NMR) relaxometry in hake (Merluccius merluccius, L.) muscle after different freezing and storage conditionsSánchez Alonso, IsabelMoreno, PilarCareche, MercedesFreezingFrozen storageLow field NMRIce crystalQualityFishMuscleLow field NMR T2 transverse relaxation measurements were performed on muscle samples from sixty hake (Merluccius merluccius, L.). Fish fillets from hake stored in ice for 3 and 14 days were subjected to different freezing methods (air blast, liquid nitrogen or walk-in freezer) and storage conditions ( 20 and 10 C for 5 days, 8 and 18 weeks). Distribution analysis of T2 data of unfrozen muscle displayed a major band (T21), accounting for 90–92% of the total signal, with a relaxation time centred at 47–60 ms and a broad band with protons of higher mobility between 300 and 800 ms, accounting for 3–5% of the signal. Upon freezing, T21 became wider and an extra band appeared within the range 120–360 ms. Whereas no changes were detected at 20 C, the T21 time constant decreased during frozen storage at 10 C in a similar way for all three freezing methods. The relative abundance of T21 declined with storage time but differences were found as a function of freezing. Results are discussed in the light of morphological alterations and protein denaturation, and it is concluded that LF NMR relaxometry is sensitive to different freezing and frozen storage conditions which can have important implications for the quality of fish muscle.This work was financed by the Spanish Ministry of Economy and Competitiveness (AGL2007-65661 and AGL2009-12485-C03-01).Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201420142014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/93006reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.foodchem.2013.12.060https://doi.org/10.1016/j.foodchem.2013.12.060Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/930062026-05-22T06:33:51Z
dc.title.none.fl_str_mv Low field nuclear magnetic resonance (LF-NMR) relaxometry in hake (Merluccius merluccius, L.) muscle after different freezing and storage conditions
title Low field nuclear magnetic resonance (LF-NMR) relaxometry in hake (Merluccius merluccius, L.) muscle after different freezing and storage conditions
spellingShingle Low field nuclear magnetic resonance (LF-NMR) relaxometry in hake (Merluccius merluccius, L.) muscle after different freezing and storage conditions
Sánchez Alonso, Isabel
Freezing
Frozen storage
Low field NMR
Ice crystal
Quality
Fish
Muscle
title_short Low field nuclear magnetic resonance (LF-NMR) relaxometry in hake (Merluccius merluccius, L.) muscle after different freezing and storage conditions
title_full Low field nuclear magnetic resonance (LF-NMR) relaxometry in hake (Merluccius merluccius, L.) muscle after different freezing and storage conditions
title_fullStr Low field nuclear magnetic resonance (LF-NMR) relaxometry in hake (Merluccius merluccius, L.) muscle after different freezing and storage conditions
title_full_unstemmed Low field nuclear magnetic resonance (LF-NMR) relaxometry in hake (Merluccius merluccius, L.) muscle after different freezing and storage conditions
title_sort Low field nuclear magnetic resonance (LF-NMR) relaxometry in hake (Merluccius merluccius, L.) muscle after different freezing and storage conditions
dc.creator.none.fl_str_mv Sánchez Alonso, Isabel
Moreno, Pilar
Careche, Mercedes
author Sánchez Alonso, Isabel
author_facet Sánchez Alonso, Isabel
Moreno, Pilar
Careche, Mercedes
author_role author
author2 Moreno, Pilar
Careche, Mercedes
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Freezing
Frozen storage
Low field NMR
Ice crystal
Quality
Fish
Muscle
topic Freezing
Frozen storage
Low field NMR
Ice crystal
Quality
Fish
Muscle
description Low field NMR T2 transverse relaxation measurements were performed on muscle samples from sixty hake (Merluccius merluccius, L.). Fish fillets from hake stored in ice for 3 and 14 days were subjected to different freezing methods (air blast, liquid nitrogen or walk-in freezer) and storage conditions ( 20 and 10 C for 5 days, 8 and 18 weeks). Distribution analysis of T2 data of unfrozen muscle displayed a major band (T21), accounting for 90–92% of the total signal, with a relaxation time centred at 47–60 ms and a broad band with protons of higher mobility between 300 and 800 ms, accounting for 3–5% of the signal. Upon freezing, T21 became wider and an extra band appeared within the range 120–360 ms. Whereas no changes were detected at 20 C, the T21 time constant decreased during frozen storage at 10 C in a similar way for all three freezing methods. The relative abundance of T21 declined with storage time but differences were found as a function of freezing. Results are discussed in the light of morphological alterations and protein denaturation, and it is concluded that LF NMR relaxometry is sensitive to different freezing and frozen storage conditions which can have important implications for the quality of fish muscle.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/93006
url http://hdl.handle.net/10261/93006
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.foodchem.2013.12.060
https://doi.org/10.1016/j.foodchem.2013.12.060
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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