Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection

This work focuses on the development of an electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection. Gold nanomaterials (AuNMs), specifically, a mixture of gold nanotriangles (AuNTs) and gold nanoparticles (AuNPs), are used to modified disposable electrodes that serve as an impr...

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Detalles Bibliográficos
Autores: Gutiérrez Gálvez, Laura, Del Caño Ochoa, Rafael, Menéndez Luque, Iris, García Nieto, Daniel, Rodríguez-Peña, Micaela, Luna, Mónica, Pineda, Teresa, Pariente Alonso, Félix, García Mendiola, Tania, Lorenzo Abad, Encarnación
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/719474
Acceso en línea:http://hdl.handle.net/10486/719474
https://dx.doi.org/10.1016/j.talanta.2021.123203
Access Level:acceso abierto
Palabra clave:AuNMs
DNA biosensor
SARS-CoV-2
Química
Descripción
Sumario:This work focuses on the development of an electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection. Gold nanomaterials (AuNMs), specifically, a mixture of gold nanotriangles (AuNTs) and gold nanoparticles (AuNPs), are used to modified disposable electrodes that serve as an improved nanostructured electrochemiluminescent platform for DNA detection. Carbon nanodots (CDs), prepared by green chemistry, are used as coreactants agents in the [Ru(bpy)3]2+ anodic electrochemiluminescence (ECL) and the hybridization is detected by changes in the ECL signal of [Ru(bpy)3]2+/CDs in combination with AuNMs nanostructures. The biosensor is shown to detect a DNA sequence corresponding to SARS-CoV-2 with a detection limit of 514 aM