Methylene Blue functionalized carbon nanodots combined with different shape gold nanostructures for sensitive and selective SARS-CoV-2 sensing

The development of DNA-sensing platforms based on new synthetized Methylene Blue functionalized carbon nanodots combined with different shape gold nanostructures (AuNs), as a new pathway to develop a selective and sensitive methodology for SARS-CoV-2 detection is presented. A mixture of gold nanopar...

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Detalles Bibliográficos
Autores: Pina-Coronado, Clara, Martínez-Sobrino, Álvaro, Gutiérrez-Gálvez, Laura, Caño, Rafael del, Martínez-Periñán, Emiliano, García-Nieto, Daniel, Rodríguez-Peña, Micaela, Luna, Mónica, Milán-Rois, Paula, Castellanos, Milagros, Abreu, Melanie, Cantón, Rafael, Galán, Juan Carlos, Pineda, Teresa, Pariente, Félix, Somoza, Álvaro, García-Mendiola, Tania, Miranda, Rodolfo, Lorenzo, Encarnación
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/280491
Acceso en línea:http://hdl.handle.net/10261/280491
Access Level:acceso abierto
Palabra clave:SARS-CoV-2
AuNs
MB-CDs
Carbon nanodots
DNA biosensor
Descripción
Sumario:The development of DNA-sensing platforms based on new synthetized Methylene Blue functionalized carbon nanodots combined with different shape gold nanostructures (AuNs), as a new pathway to develop a selective and sensitive methodology for SARS-CoV-2 detection is presented. A mixture of gold nanoparticles and gold nanotriangles have been synthetized to modify disposable electrodes that act as an enhanced nanostructured electrochemical surface for DNA probe immobilization. On the other hand, modified carbon nanodots prepared a la carte to contain Methylene Blue (MB-CDs) are used as electrochemical indicators of the hybridization event. These MB-CDs, due to their structure, are able to interact differently with double and single-stranded DNA molecules. Based on this strategy, target sequences of the SARS-CoV-2 virus have been detected in a straightforward way and rapidly with a detection limit of 2.00 aM. Moreover, this platform allows the detection of the SARS-CoV-2 sequence in the presence of other viruses, and also a single nucleotide polymorphism (SNPs). The developed approach has been tested directly on RNA obtained from nasopharyngeal samples from COVID-19 patients, avoiding any amplification process. The results agree well with those obtained by RT-qPCR or reverse transcription quantitative polymerase chain reaction technique.

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