PEPVAC: a web server for multi-epitope vaccine development based on the prediction of supertypic MHC ligands

Prediction of peptide binding to major histocompatibility complex (MHC) molecules is a basis for anticipating T-cell epitopes, as well as epitope discovery-driven vaccine development. In the human, MHC molecules are known as human leukocyte antigens (HLAs) and are extremely polymorphic. HLA polymorp...

ver descrição completa

Detalhes bibliográficos
Autores: Reche Gallardo, Pedro Antonio, Reinherz, Ellis L
Formato: artículo
Fecha de publicación:2005
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/50384
Acesso em linha:https://hdl.handle.net/20.500.14352/50384
Access Level:acceso abierto
Palavra-chave:Inmunología
Biología molecular (Biología)
Bioinformática
2412 Inmunología
2415 Biología Molecular
id ES_d26cdf7d31d0df368d2508a4edaceaa5
oai_identifier_str oai:docta.ucm.es:20.500.14352/50384
network_acronym_str ES
network_name_str España
repository_id_str
spelling PEPVAC: a web server for multi-epitope vaccine development based on the prediction of supertypic MHC ligandsReche Gallardo, Pedro AntonioReinherz, Ellis LInmunologíaBiología molecular (Biología)Bioinformática2412 Inmunología2415 Biología MolecularPrediction of peptide binding to major histocompatibility complex (MHC) molecules is a basis for anticipating T-cell epitopes, as well as epitope discovery-driven vaccine development. In the human, MHC molecules are known as human leukocyte antigens (HLAs) and are extremely polymorphic. HLA polymorphism is the basis of differential peptide binding, until now limiting the practical use of current epitope-prediction tools for vaccine development. Here, we describe a web server, PEPVAC (Promiscuous EPitope-based VACcine), optimized for the formulation of multi-epitope vaccines with broad population coverage. This optimization is accomplished through the prediction of peptides that bind to several HLA molecules with similar peptide-binding specificity (supertypes). Specifically, we offer the possibility of identifying promiscuous peptide binders to five distinct HLA class I supertypes (A2, A3, B7, A24 and B15). We estimated the phenotypic population frequency of these supertypes to be 95%, regardless of ethnicity. Targeting these supertypes for promiscuous peptide-binding predictions results in a limited number of potential epitopes without compromising the population coverage required for practical vaccine design considerations. PEPVAC can also identify conserved MHC ligands, as well as those with a C-terminus resulting from proteasomal cleavage. The combination of these features with the prediction of promiscuous HLA class I ligands further limits the number of potential epitopes. The PEPVAC server is hosted by the Dana-Farber Cancer Institute at the site http://immunax.dfci.harvard.edu/PEPVAC/.Universidad Complutense de Madrid20052005-01-0120052005-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/50384reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/503842026-06-02T12:44:21Z
dc.title.none.fl_str_mv PEPVAC: a web server for multi-epitope vaccine development based on the prediction of supertypic MHC ligands
title PEPVAC: a web server for multi-epitope vaccine development based on the prediction of supertypic MHC ligands
spellingShingle PEPVAC: a web server for multi-epitope vaccine development based on the prediction of supertypic MHC ligands
Reche Gallardo, Pedro Antonio
Inmunología
Biología molecular (Biología)
Bioinformática
2412 Inmunología
2415 Biología Molecular
title_short PEPVAC: a web server for multi-epitope vaccine development based on the prediction of supertypic MHC ligands
title_full PEPVAC: a web server for multi-epitope vaccine development based on the prediction of supertypic MHC ligands
title_fullStr PEPVAC: a web server for multi-epitope vaccine development based on the prediction of supertypic MHC ligands
title_full_unstemmed PEPVAC: a web server for multi-epitope vaccine development based on the prediction of supertypic MHC ligands
title_sort PEPVAC: a web server for multi-epitope vaccine development based on the prediction of supertypic MHC ligands
dc.creator.none.fl_str_mv Reche Gallardo, Pedro Antonio
Reinherz, Ellis L
author Reche Gallardo, Pedro Antonio
author_facet Reche Gallardo, Pedro Antonio
Reinherz, Ellis L
author_role author
author2 Reinherz, Ellis L
author2_role author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv Inmunología
Biología molecular (Biología)
Bioinformática
2412 Inmunología
2415 Biología Molecular
topic Inmunología
Biología molecular (Biología)
Bioinformática
2412 Inmunología
2415 Biología Molecular
description Prediction of peptide binding to major histocompatibility complex (MHC) molecules is a basis for anticipating T-cell epitopes, as well as epitope discovery-driven vaccine development. In the human, MHC molecules are known as human leukocyte antigens (HLAs) and are extremely polymorphic. HLA polymorphism is the basis of differential peptide binding, until now limiting the practical use of current epitope-prediction tools for vaccine development. Here, we describe a web server, PEPVAC (Promiscuous EPitope-based VACcine), optimized for the formulation of multi-epitope vaccines with broad population coverage. This optimization is accomplished through the prediction of peptides that bind to several HLA molecules with similar peptide-binding specificity (supertypes). Specifically, we offer the possibility of identifying promiscuous peptide binders to five distinct HLA class I supertypes (A2, A3, B7, A24 and B15). We estimated the phenotypic population frequency of these supertypes to be 95%, regardless of ethnicity. Targeting these supertypes for promiscuous peptide-binding predictions results in a limited number of potential epitopes without compromising the population coverage required for practical vaccine design considerations. PEPVAC can also identify conserved MHC ligands, as well as those with a C-terminus resulting from proteasomal cleavage. The combination of these features with the prediction of promiscuous HLA class I ligands further limits the number of potential epitopes. The PEPVAC server is hosted by the Dana-Farber Cancer Institute at the site http://immunax.dfci.harvard.edu/PEPVAC/.
publishDate 2005
dc.date.none.fl_str_mv 2005
2005-01-01
2005
2005-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/50384
url https://hdl.handle.net/20.500.14352/50384
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869420371246055424
score 15,300719

Registos relacionados