Evaluation of hydrophilic and lipophilic antioxidant capacity in Spanish tomato paste: usefulness of front-face total fluroescence signal combined with PARAFAC

The hydrophilic and lipophilic antioxidant activities due to the main bioactive components present in Spanish tomato paste samples were studied, using standardized and fluorescent methods. After extraction, phenolic antioxidants (Folin-Ciocalteu method) and total antioxidant activity (TEAC assay) we...

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Detalles Bibliográficos
Autores: Pardo Botello, Rosario, Chamizo Calero, Fátima, Rodríguez Corchado, Raquel, Torre Carreras, Rosa de la, Galeano Díaz, Teresa, Monago Maraña, Olga
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Nacional de Educación a Distancia
Repositorio:e-spacio. Repositorio Institucional de la UNED
Idioma:inglés
OAI Identifier:oai:e-spacio.uned.es:20.500.14468/11567
Acceso en línea:https://hdl.handle.net/20.500.14468/11567
Access Level:acceso abierto
Palabra clave:tomatoes
lipophilic and hydrophilic antioxidant activities
Folin-Ciocalteu and TEAC assays
front-face fluorescence
excitation-emission matrices-PARAFAC
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oai_identifier_str oai:e-spacio.uned.es:20.500.14468/11567
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repository_id_str
spelling Evaluation of hydrophilic and lipophilic antioxidant capacity in Spanish tomato paste: usefulness of front-face total fluroescence signal combined with PARAFACPardo Botello, RosarioChamizo Calero, FátimaRodríguez Corchado, RaquelTorre Carreras, Rosa de laGaleano Díaz, TeresaMonago Maraña, Olgatomatoeslipophilic and hydrophilic antioxidant activitiesFolin-Ciocalteu and TEAC assaysfront-face fluorescenceexcitation-emission matrices-PARAFACThe hydrophilic and lipophilic antioxidant activities due to the main bioactive components present in Spanish tomato paste samples were studied, using standardized and fluorescent methods. After extraction, phenolic antioxidants (Folin-Ciocalteu method) and total antioxidant activity (TEAC assay) were evaluated, examining differences between hydrophilic and lipophilic extracts corresponding to different samples. Total fluorescence spectra of extracts (excitation-emission matrices, EEMs) were recorded in the front-face mode at two different ranges: 210-300 nm/ 310-390 nm, and 295-350 nm/380-480 nm, for excitation and emission, respectively, in the hydrophilic extracts. In the lipophilic extracts, the first range was 230-283 nm/290-340 nm, while the second range was 315-383 nm/390-500 nm for excitation and emission, respectively. EEMs from a set of 22 samples were analyzed by the second-order multivariate technique Parallel Factor Analysis (PARAFAC). Tentative assignation of the different components to the various fluorophores of tomato was tried, based on literature. Correlation between the antioxidant activity and score values retrieved for different components in PARAFAC model was obtained. The possibility of using EEMs-PARAFAC to evaluate antioxidant activity of hydrophilic and lipophilic compounds in these samples was examined, obtaining good results in accordance with the Folin-Ciocalteu and TEAC assays.Springere-Spacio UNED20242024-05-2020212021-12-0120212021-12-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14468/11567reponame:e-spacio. Repositorio Institucional de la UNEDinstname:Universidad Nacional de Educación a DistanciaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0oai:e-spacio.uned.es:20.500.14468/115672026-06-06T12:38:31Z
dc.title.none.fl_str_mv Evaluation of hydrophilic and lipophilic antioxidant capacity in Spanish tomato paste: usefulness of front-face total fluroescence signal combined with PARAFAC
title Evaluation of hydrophilic and lipophilic antioxidant capacity in Spanish tomato paste: usefulness of front-face total fluroescence signal combined with PARAFAC
spellingShingle Evaluation of hydrophilic and lipophilic antioxidant capacity in Spanish tomato paste: usefulness of front-face total fluroescence signal combined with PARAFAC
Pardo Botello, Rosario
tomatoes
lipophilic and hydrophilic antioxidant activities
Folin-Ciocalteu and TEAC assays
front-face fluorescence
excitation-emission matrices-PARAFAC
title_short Evaluation of hydrophilic and lipophilic antioxidant capacity in Spanish tomato paste: usefulness of front-face total fluroescence signal combined with PARAFAC
title_full Evaluation of hydrophilic and lipophilic antioxidant capacity in Spanish tomato paste: usefulness of front-face total fluroescence signal combined with PARAFAC
title_fullStr Evaluation of hydrophilic and lipophilic antioxidant capacity in Spanish tomato paste: usefulness of front-face total fluroescence signal combined with PARAFAC
title_full_unstemmed Evaluation of hydrophilic and lipophilic antioxidant capacity in Spanish tomato paste: usefulness of front-face total fluroescence signal combined with PARAFAC
title_sort Evaluation of hydrophilic and lipophilic antioxidant capacity in Spanish tomato paste: usefulness of front-face total fluroescence signal combined with PARAFAC
dc.creator.none.fl_str_mv Pardo Botello, Rosario
Chamizo Calero, Fátima
Rodríguez Corchado, Raquel
Torre Carreras, Rosa de la
Galeano Díaz, Teresa
Monago Maraña, Olga
author Pardo Botello, Rosario
author_facet Pardo Botello, Rosario
Chamizo Calero, Fátima
Rodríguez Corchado, Raquel
Torre Carreras, Rosa de la
Galeano Díaz, Teresa
Monago Maraña, Olga
author_role author
author2 Chamizo Calero, Fátima
Rodríguez Corchado, Raquel
Torre Carreras, Rosa de la
Galeano Díaz, Teresa
Monago Maraña, Olga
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv e-Spacio UNED
dc.subject.none.fl_str_mv tomatoes
lipophilic and hydrophilic antioxidant activities
Folin-Ciocalteu and TEAC assays
front-face fluorescence
excitation-emission matrices-PARAFAC
topic tomatoes
lipophilic and hydrophilic antioxidant activities
Folin-Ciocalteu and TEAC assays
front-face fluorescence
excitation-emission matrices-PARAFAC
description The hydrophilic and lipophilic antioxidant activities due to the main bioactive components present in Spanish tomato paste samples were studied, using standardized and fluorescent methods. After extraction, phenolic antioxidants (Folin-Ciocalteu method) and total antioxidant activity (TEAC assay) were evaluated, examining differences between hydrophilic and lipophilic extracts corresponding to different samples. Total fluorescence spectra of extracts (excitation-emission matrices, EEMs) were recorded in the front-face mode at two different ranges: 210-300 nm/ 310-390 nm, and 295-350 nm/380-480 nm, for excitation and emission, respectively, in the hydrophilic extracts. In the lipophilic extracts, the first range was 230-283 nm/290-340 nm, while the second range was 315-383 nm/390-500 nm for excitation and emission, respectively. EEMs from a set of 22 samples were analyzed by the second-order multivariate technique Parallel Factor Analysis (PARAFAC). Tentative assignation of the different components to the various fluorophores of tomato was tried, based on literature. Correlation between the antioxidant activity and score values retrieved for different components in PARAFAC model was obtained. The possibility of using EEMs-PARAFAC to evaluate antioxidant activity of hydrophilic and lipophilic compounds in these samples was examined, obtaining good results in accordance with the Folin-Ciocalteu and TEAC assays.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-12-01
2021
2021-12-01
2024
2024-05-20
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14468/11567
url https://hdl.handle.net/20.500.14468/11567
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
http://creativecommons.org/licenses/by-nc-nd/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:e-spacio. Repositorio Institucional de la UNED
instname:Universidad Nacional de Educación a Distancia
instname_str Universidad Nacional de Educación a Distancia
reponame_str e-spacio. Repositorio Institucional de la UNED
collection e-spacio. Repositorio Institucional de la UNED
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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