Caco-2 cell response induced by peptides released after digestion of heat-treated egg white proteins

This article belongs to the Special Issue Molecular Approaches for Food Protein Allergenicity Assesment and the Diagnosis and Treatment of Food Allergies.

Detalhes bibliográficos
Autores: Benedé, Sara, Pérez-Rodríguez, Leticia, Molina, Elena
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/297187
Acesso em linha:http://hdl.handle.net/10261/297187
Access Level:acceso abierto
Palavra-chave:Ovalbumin
Ovomucoid
Lysozyme
Caco-2 cells
Heat treatment
Digestion
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spelling Caco-2 cell response induced by peptides released after digestion of heat-treated egg white proteinsBenedé, SaraPérez-Rodríguez, LeticiaMolina, ElenaOvalbuminOvomucoidLysozymeCaco-2 cellsHeat treatmentDigestionThis article belongs to the Special Issue Molecular Approaches for Food Protein Allergenicity Assesment and the Diagnosis and Treatment of Food Allergies.The heat treatment of food proteins induces structural modifications that influence their interaction with human fluids and cells. We aimed to evaluate the Caco-2 cell response induced by peptides produced after digestion of heat-treated egg white proteins. In vitro digestion of ovalbumin (OVA), ovomucoid (OM), and lysozyme (LYS), untreated or previously heated, was performed. The digestibility of proteins and the response of Caco-2 cells exposed to peptides (<10 kDa) generated during digestion were evaluated. Intact OVA and LYS persisted after the digestion of native proteins, whereas OM was completely hydrolysed. A heat treatment at 65 °C for 30 min did not alter the digestibility of OVA, whereas at 90 °C for 3 min, protein degradation was favoured. The digestibility of OM and LYS was not affected by heat treatment. Peptides derived from OVA and OM digestion induced IL-6 and IL-8 production. OVA and LYS digestion promoted the expression of Tslp, and Il6 and Il33, respectively. A heat treatment prior to OVA digestion reduced IL-6 production and Tslp expression. It was concluded that heat treatments can reduce the release of OVA-derived peptides, but not OM and LYS, with proinflammatory activity during digestion.This research was funded by the Ministerio de Ciencia, Innovación y Universidades (project AGL2017-88964-R). L.P.-R. was funded by an FPU grant (Ref. FPU16/01974) from the Ministerio de Educación, Cultura y Deporte.Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Ministerio de Educación, Cultura y Deporte (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/297187reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88964-RFoodshttps://doi.org/10.3390/foods11223566Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2971872026-05-22T06:33:51Z
dc.title.none.fl_str_mv Caco-2 cell response induced by peptides released after digestion of heat-treated egg white proteins
title Caco-2 cell response induced by peptides released after digestion of heat-treated egg white proteins
spellingShingle Caco-2 cell response induced by peptides released after digestion of heat-treated egg white proteins
Benedé, Sara
Ovalbumin
Ovomucoid
Lysozyme
Caco-2 cells
Heat treatment
Digestion
title_short Caco-2 cell response induced by peptides released after digestion of heat-treated egg white proteins
title_full Caco-2 cell response induced by peptides released after digestion of heat-treated egg white proteins
title_fullStr Caco-2 cell response induced by peptides released after digestion of heat-treated egg white proteins
title_full_unstemmed Caco-2 cell response induced by peptides released after digestion of heat-treated egg white proteins
title_sort Caco-2 cell response induced by peptides released after digestion of heat-treated egg white proteins
dc.creator.none.fl_str_mv Benedé, Sara
Pérez-Rodríguez, Leticia
Molina, Elena
author Benedé, Sara
author_facet Benedé, Sara
Pérez-Rodríguez, Leticia
Molina, Elena
author_role author
author2 Pérez-Rodríguez, Leticia
Molina, Elena
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Ministerio de Educación, Cultura y Deporte (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Ovalbumin
Ovomucoid
Lysozyme
Caco-2 cells
Heat treatment
Digestion
topic Ovalbumin
Ovomucoid
Lysozyme
Caco-2 cells
Heat treatment
Digestion
description This article belongs to the Special Issue Molecular Approaches for Food Protein Allergenicity Assesment and the Diagnosis and Treatment of Food Allergies.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/297187
url http://hdl.handle.net/10261/297187
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88964-R
Foods
https://doi.org/10.3390/foods11223566
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869420255012454400
score 15,812429

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