Validación de métodos de cribado para la detección de antibióticos en lactosuero de cabra
The presence of residues of antibiotics in milk from animals that have been treated with veterinary medicinal products either therapeutic or prophylactic without respecting the good practices of use, can have serious consequences for public health and the dairy sector. In the case of the manufacture...
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| Tipo de recurso: | tesis de maestría |
| Fecha de publicación: | 2017 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | español |
| OAI Identifier: | oai:riunet.upv.es:10251/87168 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/87168 |
| Access Level: | acceso abierto |
| Palabra clave: | Mètodes de cribatge Goat milk Whey Antibiotics Screening tests Métodos de cribado Antibióticos Lactosuero Leche de cabra Antibiòtic Lactosèrum Llet de cabra PRODUCCION ANIMAL Máster Universitario en Producción Animal-Màster Universitari en Producció Animal |
| Sumario: | The presence of residues of antibiotics in milk from animals that have been treated with veterinary medicinal products either therapeutic or prophylactic without respecting the good practices of use, can have serious consequences for public health and the dairy sector. In the case of the manufacture of cheese, depending on the nature of the antibiotic it can be held mostly in curd or be disposed with the whey. Must be borne in mind that whey can be used in animal feed or employee for the manufacture of other products (curd, concentrated of protein, whey powder, etc.) by which the presence of residues of antibiotics in whey may represent a risk to food safety and animal health, as well as for the environment making it necessary to have a control system. Screening methods for detection of antibiotics are currently available from a commercial standpoint in milk which may be microbiological type that are non-specific or binding to protein receptors that are specific for the detection of one, two or several families of antibiotics. All of these methods have been validated for use in milk of different species but not for use in whey from the cheese making. Therefore, the objective of this work has been the validation criteria of Decision 2002/657/EC of microbiological method Eclipse Farm 3 G® coupled to the team e - Reader® and 3 screening methods of binding to receptors (TwinSensor®, 3AminoSensor® and QuinoSensor®) for the detection of antibiotics used in dairy goats. The whey samples from experimental elaborations of cheese from goat's milk were used without antibiotics and also fortified with different concentrations of antibiotics (amoxicillin, penicillin, cephalexin, oxytetracycline, gentamicin, tylosin, sullfatiazol and enrofloxacin) and then were analysed using the screening methods. The results for the Eclipse Farm method 3G® coupled to e - Reader® indicates that it is necessary to samples predifusion for one hour at room temperature when whey is analyzed. The method presented with this array of very high specificity (97.7%) indicating a low rate of false-positive results. In addition the method is suitable for the detection of ß-lactam antibiotics (except for amoxicillin), aminoglycosides and tetracyclines presenting cscarce sensitivity for the detection of quinolones. In the case of methods of receptors binding is necessary to elongated the time of incubation in the analysis of whey, presenting the TwinSensor® high detection capability for ß-lactam (except for Cephalexin) and tetracyclines, as it was also adequate found with QuinoSensor® for enrofloxacin, while 3 AminoSensor® showed a less sensitivity in the detection of gentamicin. In all cases the methods of receptor binding show a high specificity (100%). In conclusion, the commercial screening methods using specific analysis protocol could be an appropriate tool in the establishment of a control system for the presence of antibiotics in the whey. |
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