Comprehensive analysis of sperm DNA fragmentation by five different assays

Summary: Sperm DNA fragmentation (SDF) is becoming an important test to assess male infertility. Several different tests are available, but no consensus has yet been reached as to which tests are most predictive of infertility. Few publications have reported a comprehensive analysis comparing these...

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Autores: Ribas-Maynou, Jordi|||0000-0002-9101-2044, García-Peiró, Agustí, Fernandez Encinas, Alba|||0000-0003-2701-4739, Abad, Carlos|||0000-0002-2703-4062, Amengual Guedan, Maria Jose|||0000-0002-5385-3061, Prada, E., Navarro, J., Benet i Català, Jordi|||0000-0001-6185-2555
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:307190
Acceso en línea:https://ddd.uab.cat/record/307190
https://dx.doi.org/urn:doi:10.1111/j.2047-2927.2013.00111.x
Access Level:acceso abierto
Palabra clave:Comet assay
DNA damage
Infertility
Male infertility
SCD test
SCSA
TUNEL assay
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spelling Comprehensive analysis of sperm DNA fragmentation by five different assaysTUNEL assay, SCSA, SCD test and alkaline and neutral Comet assayRibas-Maynou, Jordi|||0000-0002-9101-2044García-Peiró, AgustíFernandez Encinas, Alba|||0000-0003-2701-4739Abad, Carlos|||0000-0002-2703-4062Amengual Guedan, Maria Jose|||0000-0002-5385-3061Prada, E.Navarro, J.Benet i Català, Jordi|||0000-0001-6185-2555Comet assayDNA damageInfertilityMale infertilitySCD testSCSATUNEL assaySummary: Sperm DNA fragmentation (SDF) is becoming an important test to assess male infertility. Several different tests are available, but no consensus has yet been reached as to which tests are most predictive of infertility. Few publications have reported a comprehensive analysis comparing these methods within the same population. The objective of this study was to analyze the differences between the five most common methodologies, to study their correlations and to establish their cut-off values, sensitivity and specificity in predicting male infertility. We found differences in SDF between fertile donors and infertile patients in TUNEL, SCSA, SCD and alkaline Comet assays, but none with the neutral Comet assay. The alkaline COMET assay was the best in predicting male infertility followed by TUNEL, SCD and SCSA, whereas the neutral COMET assay had no predictive power. For our patient population, threshold values for infertility were 20.05% for TUNEL assay, 18.90% for SCSA, 22.75% for the SCD test, 45.37% for alkaline Comet and 34.37% for neutral Comet. This work establishes in a comprehensive study that the all techniques except neutral Comet are useful to distinguish fertile and infertile men. © 2013 American Society of Andrology and European Academy of Andrology. 22013-01-0120132013-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/307190https://dx.doi.org/urn:doi:10.1111/j.2047-2927.2013.00111.xreponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengInstituto de Salud Carlos III https://doi.org/10.13039/501100004587 FISPI11/00630Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2009/SGR-0110open accesshttp://purl.org/coar/access_right/c_abf2Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.https://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3071902026-06-06T12:50:31Z
dc.title.none.fl_str_mv Comprehensive analysis of sperm DNA fragmentation by five different assays
TUNEL assay, SCSA, SCD test and alkaline and neutral Comet assay
title Comprehensive analysis of sperm DNA fragmentation by five different assays
spellingShingle Comprehensive analysis of sperm DNA fragmentation by five different assays
Ribas-Maynou, Jordi|||0000-0002-9101-2044
Comet assay
DNA damage
Infertility
Male infertility
SCD test
SCSA
TUNEL assay
title_short Comprehensive analysis of sperm DNA fragmentation by five different assays
title_full Comprehensive analysis of sperm DNA fragmentation by five different assays
title_fullStr Comprehensive analysis of sperm DNA fragmentation by five different assays
title_full_unstemmed Comprehensive analysis of sperm DNA fragmentation by five different assays
title_sort Comprehensive analysis of sperm DNA fragmentation by five different assays
dc.creator.none.fl_str_mv Ribas-Maynou, Jordi|||0000-0002-9101-2044
García-Peiró, Agustí
Fernandez Encinas, Alba|||0000-0003-2701-4739
Abad, Carlos|||0000-0002-2703-4062
Amengual Guedan, Maria Jose|||0000-0002-5385-3061
Prada, E.
Navarro, J.
Benet i Català, Jordi|||0000-0001-6185-2555
author Ribas-Maynou, Jordi|||0000-0002-9101-2044
author_facet Ribas-Maynou, Jordi|||0000-0002-9101-2044
García-Peiró, Agustí
Fernandez Encinas, Alba|||0000-0003-2701-4739
Abad, Carlos|||0000-0002-2703-4062
Amengual Guedan, Maria Jose|||0000-0002-5385-3061
Prada, E.
Navarro, J.
Benet i Català, Jordi|||0000-0001-6185-2555
author_role author
author2 García-Peiró, Agustí
Fernandez Encinas, Alba|||0000-0003-2701-4739
Abad, Carlos|||0000-0002-2703-4062
Amengual Guedan, Maria Jose|||0000-0002-5385-3061
Prada, E.
Navarro, J.
Benet i Català, Jordi|||0000-0001-6185-2555
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Comet assay
DNA damage
Infertility
Male infertility
SCD test
SCSA
TUNEL assay
topic Comet assay
DNA damage
Infertility
Male infertility
SCD test
SCSA
TUNEL assay
description Summary: Sperm DNA fragmentation (SDF) is becoming an important test to assess male infertility. Several different tests are available, but no consensus has yet been reached as to which tests are most predictive of infertility. Few publications have reported a comprehensive analysis comparing these methods within the same population. The objective of this study was to analyze the differences between the five most common methodologies, to study their correlations and to establish their cut-off values, sensitivity and specificity in predicting male infertility. We found differences in SDF between fertile donors and infertile patients in TUNEL, SCSA, SCD and alkaline Comet assays, but none with the neutral Comet assay. The alkaline COMET assay was the best in predicting male infertility followed by TUNEL, SCD and SCSA, whereas the neutral COMET assay had no predictive power. For our patient population, threshold values for infertility were 20.05% for TUNEL assay, 18.90% for SCSA, 22.75% for the SCD test, 45.37% for alkaline Comet and 34.37% for neutral Comet. This work establishes in a comprehensive study that the all techniques except neutral Comet are useful to distinguish fertile and infertile men. © 2013 American Society of Andrology and European Academy of Andrology.
publishDate 2013
dc.date.none.fl_str_mv 2
2013-01-01
2013
2013-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/307190
https://dx.doi.org/urn:doi:10.1111/j.2047-2927.2013.00111.x
url https://ddd.uab.cat/record/307190
https://dx.doi.org/urn:doi:10.1111/j.2047-2927.2013.00111.x
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 FISPI11/00630
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2009/SGR-0110
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
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https://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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