Field Crop Evaluation of Polymeric Nanoparticles of Garlic Extract–Chitosan as Biostimulant Seed Nano-Priming in Cereals and Transcriptomic Insights

Current crop management worldwide is shifting toward the use of environmentally friendly products. With this objective, we developed a new phytosanitary product with biostimulant properties based on the encapsulation of garlic extract at a lower dose (<0.1%) in chitosan nanoparticles as a seed na...

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Autores: Mondéjar López, María, López Jiménez, Alberto José, Gómez Gómez, María Lourdes, Ahrazem El Kadiri, Oussama, García Martínez, Joaquín Calixto, Niza González, Enrique
Formato: artículo
Fecha de publicación:2024
País:España
Recursos:Universidad de Castilla-La Mancha
Repositorio:RUIdeRA. Repositorio Institucional de la UCLM
OAI Identifier:oai:ruidera.uclm.es:10578/39704
Acesso em linha:https://doi.org/10.3390/polym16233385
https://hdl.handle.net/10578/39704
Access Level:acceso abierto
Palavra-chave:Biostimulant properties
Chitosan nanoparticles
Crop management
Garlic extract
Transcriptomic analysis
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spelling Field Crop Evaluation of Polymeric Nanoparticles of Garlic Extract–Chitosan as Biostimulant Seed Nano-Priming in Cereals and Transcriptomic InsightsMondéjar López, MaríaLópez Jiménez, Alberto JoséGómez Gómez, María LourdesAhrazem El Kadiri, OussamaGarcía Martínez, Joaquín CalixtoNiza González, EnriqueBiostimulant propertiesChitosan nanoparticlesCrop managementGarlic extractTranscriptomic analysisCurrent crop management worldwide is shifting toward the use of environmentally friendly products. With this objective, we developed a new phytosanitary product with biostimulant properties based on the encapsulation of garlic extract at a lower dose (<0.1%) in chitosan nanoparticles as a seed nano-priming agent. In the present study, the morphology of the nanoparticles, their stability under prolonged storage conditions, and their efficacy as a biostimulant are evaluated on cereals in rainfed crops, and the activities were correlated with a transcriptomic analysis. The nanoparticles showed a spherical shape and had a maximum size close to 200 nm with satisfactory stability at 4 °C, reducing the probability of aggregation processes in the nanoparticles. The biostimulant properties of the nano-priming agent were evaluated in a field experiment with wheat, barley, and oat seeds at 30 and 90 days, showing that plants treated with nanoparticles showed significant differences with higher values in root development, leaf length, and total plant weight. Finally, through a RNA-SEQ analysis of the treated wheat seeds, we have confirmed that the nano-treatment showed a higher increases in regard to development, metabolism, and plant response genes compared with untreated seeds.MDPI202420242024info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://doi.org/10.3390/polym16233385https://hdl.handle.net/10578/39704reponame:RUIdeRA. Repositorio Institucional de la UCLMinstname:Universidad de Castilla-La ManchaInglés200360UCTRinfo:eu-repo/semantics/openAccessoai:ruidera.uclm.es:10578/397042026-05-27T07:36:41Z
dc.title.none.fl_str_mv Field Crop Evaluation of Polymeric Nanoparticles of Garlic Extract–Chitosan as Biostimulant Seed Nano-Priming in Cereals and Transcriptomic Insights
title Field Crop Evaluation of Polymeric Nanoparticles of Garlic Extract–Chitosan as Biostimulant Seed Nano-Priming in Cereals and Transcriptomic Insights
spellingShingle Field Crop Evaluation of Polymeric Nanoparticles of Garlic Extract–Chitosan as Biostimulant Seed Nano-Priming in Cereals and Transcriptomic Insights
Mondéjar López, María
Biostimulant properties
Chitosan nanoparticles
Crop management
Garlic extract
Transcriptomic analysis
title_short Field Crop Evaluation of Polymeric Nanoparticles of Garlic Extract–Chitosan as Biostimulant Seed Nano-Priming in Cereals and Transcriptomic Insights
title_full Field Crop Evaluation of Polymeric Nanoparticles of Garlic Extract–Chitosan as Biostimulant Seed Nano-Priming in Cereals and Transcriptomic Insights
title_fullStr Field Crop Evaluation of Polymeric Nanoparticles of Garlic Extract–Chitosan as Biostimulant Seed Nano-Priming in Cereals and Transcriptomic Insights
title_full_unstemmed Field Crop Evaluation of Polymeric Nanoparticles of Garlic Extract–Chitosan as Biostimulant Seed Nano-Priming in Cereals and Transcriptomic Insights
title_sort Field Crop Evaluation of Polymeric Nanoparticles of Garlic Extract–Chitosan as Biostimulant Seed Nano-Priming in Cereals and Transcriptomic Insights
dc.creator.none.fl_str_mv Mondéjar López, María
López Jiménez, Alberto José
Gómez Gómez, María Lourdes
Ahrazem El Kadiri, Oussama
García Martínez, Joaquín Calixto
Niza González, Enrique
author Mondéjar López, María
author_facet Mondéjar López, María
López Jiménez, Alberto José
Gómez Gómez, María Lourdes
Ahrazem El Kadiri, Oussama
García Martínez, Joaquín Calixto
Niza González, Enrique
author_role author
author2 López Jiménez, Alberto José
Gómez Gómez, María Lourdes
Ahrazem El Kadiri, Oussama
García Martínez, Joaquín Calixto
Niza González, Enrique
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biostimulant properties
Chitosan nanoparticles
Crop management
Garlic extract
Transcriptomic analysis
topic Biostimulant properties
Chitosan nanoparticles
Crop management
Garlic extract
Transcriptomic analysis
description Current crop management worldwide is shifting toward the use of environmentally friendly products. With this objective, we developed a new phytosanitary product with biostimulant properties based on the encapsulation of garlic extract at a lower dose (<0.1%) in chitosan nanoparticles as a seed nano-priming agent. In the present study, the morphology of the nanoparticles, their stability under prolonged storage conditions, and their efficacy as a biostimulant are evaluated on cereals in rainfed crops, and the activities were correlated with a transcriptomic analysis. The nanoparticles showed a spherical shape and had a maximum size close to 200 nm with satisfactory stability at 4 °C, reducing the probability of aggregation processes in the nanoparticles. The biostimulant properties of the nano-priming agent were evaluated in a field experiment with wheat, barley, and oat seeds at 30 and 90 days, showing that plants treated with nanoparticles showed significant differences with higher values in root development, leaf length, and total plant weight. Finally, through a RNA-SEQ analysis of the treated wheat seeds, we have confirmed that the nano-treatment showed a higher increases in regard to development, metabolism, and plant response genes compared with untreated seeds.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://doi.org/10.3390/polym16233385
https://hdl.handle.net/10578/39704
url https://doi.org/10.3390/polym16233385
https://hdl.handle.net/10578/39704
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv 200360UCTR
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:RUIdeRA. Repositorio Institucional de la UCLM
instname:Universidad de Castilla-La Mancha
instname_str Universidad de Castilla-La Mancha
reponame_str RUIdeRA. Repositorio Institucional de la UCLM
collection RUIdeRA. Repositorio Institucional de la UCLM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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