PYL1-and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue
[EN] To face the challenges of climate change and sustainable food production, it is essential to develop crop genome editing techniques to pinpoint key genes involved in abiotic stress signaling. The identification of those prevailing abscisic acid (ABA) receptors that mediate plant-environment int...
| Autores: | , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/193324 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/193324 |
| Access Level: | acceso abierto |
| Palabra clave: | ABA receptors Gene editing CRISPR Cas9 Nicotiana benthamiana Biotechnological crop Drought Multiplex mutations ABA sensitivity,tetraploid,extremophile FISIOLOGIA VEGETAL 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible |
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PYL1-and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative TissuePizzio, Gaston A.Mayordomo, CristianLozano-Juste, JorgeVazquez-Vilar, MartaKaminski, Kacper P.Ivanov, Nikolai V.Estevez ,Juan C.Rivera-Moreno, MariaAlbert, ArmandoRodríguez, Pedro L.García-Carpintero-Burgos, VíctorNebauer, Sergio G.|||0000-0001-7978-6680Orzáez Calatayud, Diego VicenteABA receptorsGene editingCRISPRCas9Nicotiana benthamianaBiotechnological cropDroughtMultiplex mutationsABA sensitivity,tetraploid,extremophileFISIOLOGIA VEGETAL02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible[EN] To face the challenges of climate change and sustainable food production, it is essential to develop crop genome editing techniques to pinpoint key genes involved in abiotic stress signaling. The identification of those prevailing abscisic acid (ABA) receptors that mediate plant-environment interactions is quite challenging in polyploid plants because of the high number of genes in the PYR/PYL/RCAR ABA receptor family. Nicotiana benthamiana is a biotechnological crop amenable to genome editing, and given the importance of ABA signaling in coping with drought stress, we initiated the analysis of its 23-member family of ABA receptors through multiplex CRISPR/Cas9-mediated editing. We generated several high-order mutants impaired in NbPYL1-like and NbPYL8-like receptors, which showed certain insensitivity to ABA for inhibition of seedling establishment, growth, and development of shoot and lateral roots as well as reduced sensitivity to the PYL1-agonist cyanabactin (CB). However, in these high-order mutants, regulation of transpiration was not affected and was responsive to ABA treatment. This reveals a robust and redundant control of transpiration in this allotetraploid plant that probably reflects its origin from the extreme habitat of central Australia.This research was supported by grant PID2020-113100RB (P.L.R.) and PID2020-119805RB (A.A.) funded by MCIN/AEI/10.13039/501100011033 and by Newcotiana H2020 760331 (D.O.).MDPIDepartamento de Producción VegetalInstituto Universitario Mixto de Biología Molecular y Celular de PlantasEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalEuropean CommissionAgencia Estatal de InvestigaciónRepositorio Institucional de la Universitat Politècnica de València Riunet20222022-03-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/193324reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-113100RB-I00 RESPUESTA A ESTRES ABIOTICO MEDIANTE REGULACION DEL RECAMBIO DE RECEPTORES DE ABA POR E3 LIGASAS DEL TIPO RBR Y MEDIANTE LA ACTIVACION DE SNRK2S POR MAP4KSAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-119805RB-I00 FUNCION, ESTRUCTURA Y DISEÑO DE SENSORES MOLECULARES QUE CONTROLAN LA RESPUESTA DE LAS PLANTAS AL ESTRES POR SEQUIA Y SALEuropean Commission https://doi.org/10.13039/501100000780 H2020 760331open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1933242026-06-13T07:49:27Z |
| dc.title.none.fl_str_mv |
PYL1-and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue |
| title |
PYL1-and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue |
| spellingShingle |
PYL1-and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue Pizzio, Gaston A. ABA receptors Gene editing CRISPR Cas9 Nicotiana benthamiana Biotechnological crop Drought Multiplex mutations ABA sensitivity,tetraploid,extremophile FISIOLOGIA VEGETAL 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible |
| title_short |
PYL1-and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue |
| title_full |
PYL1-and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue |
| title_fullStr |
PYL1-and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue |
| title_full_unstemmed |
PYL1-and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue |
| title_sort |
PYL1-and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue |
| dc.creator.none.fl_str_mv |
Pizzio, Gaston A. Mayordomo, Cristian Lozano-Juste, Jorge Vazquez-Vilar, Marta Kaminski, Kacper P. Ivanov, Nikolai V. Estevez ,Juan C. Rivera-Moreno, Maria Albert, Armando Rodríguez, Pedro L. García-Carpintero-Burgos, Víctor Nebauer, Sergio G.|||0000-0001-7978-6680 Orzáez Calatayud, Diego Vicente |
| author |
Pizzio, Gaston A. |
| author_facet |
Pizzio, Gaston A. Mayordomo, Cristian Lozano-Juste, Jorge Vazquez-Vilar, Marta Kaminski, Kacper P. Ivanov, Nikolai V. Estevez ,Juan C. Rivera-Moreno, Maria Albert, Armando Rodríguez, Pedro L. García-Carpintero-Burgos, Víctor Nebauer, Sergio G.|||0000-0001-7978-6680 Orzáez Calatayud, Diego Vicente |
| author_role |
author |
| author2 |
Mayordomo, Cristian Lozano-Juste, Jorge Vazquez-Vilar, Marta Kaminski, Kacper P. Ivanov, Nikolai V. Estevez ,Juan C. Rivera-Moreno, Maria Albert, Armando Rodríguez, Pedro L. García-Carpintero-Burgos, Víctor Nebauer, Sergio G.|||0000-0001-7978-6680 Orzáez Calatayud, Diego Vicente |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Departamento de Producción Vegetal Instituto Universitario Mixto de Biología Molecular y Celular de Plantas Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural European Commission Agencia Estatal de Investigación Repositorio Institucional de la Universitat Politècnica de València Riunet |
| dc.subject.none.fl_str_mv |
ABA receptors Gene editing CRISPR Cas9 Nicotiana benthamiana Biotechnological crop Drought Multiplex mutations ABA sensitivity,tetraploid,extremophile FISIOLOGIA VEGETAL 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible |
| topic |
ABA receptors Gene editing CRISPR Cas9 Nicotiana benthamiana Biotechnological crop Drought Multiplex mutations ABA sensitivity,tetraploid,extremophile FISIOLOGIA VEGETAL 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible |
| description |
[EN] To face the challenges of climate change and sustainable food production, it is essential to develop crop genome editing techniques to pinpoint key genes involved in abiotic stress signaling. The identification of those prevailing abscisic acid (ABA) receptors that mediate plant-environment interactions is quite challenging in polyploid plants because of the high number of genes in the PYR/PYL/RCAR ABA receptor family. Nicotiana benthamiana is a biotechnological crop amenable to genome editing, and given the importance of ABA signaling in coping with drought stress, we initiated the analysis of its 23-member family of ABA receptors through multiplex CRISPR/Cas9-mediated editing. We generated several high-order mutants impaired in NbPYL1-like and NbPYL8-like receptors, which showed certain insensitivity to ABA for inhibition of seedling establishment, growth, and development of shoot and lateral roots as well as reduced sensitivity to the PYL1-agonist cyanabactin (CB). However, in these high-order mutants, regulation of transpiration was not affected and was responsive to ABA treatment. This reveals a robust and redundant control of transpiration in this allotetraploid plant that probably reflects its origin from the extreme habitat of central Australia. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 2022-03-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://riunet.upv.es/handle/10251/193324 |
| url |
https://riunet.upv.es/handle/10251/193324 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-113100RB-I00 RESPUESTA A ESTRES ABIOTICO MEDIANTE REGULACION DEL RECAMBIO DE RECEPTORES DE ABA POR E3 LIGASAS DEL TIPO RBR Y MEDIANTE LA ACTIVACION DE SNRK2S POR MAP4KS Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-119805RB-I00 FUNCION, ESTRUCTURA Y DISEÑO DE SENSORES MOLECULARES QUE CONTROLAN LA RESPUESTA DE LAS PLANTAS AL ESTRES POR SEQUIA Y SAL European Commission https://doi.org/10.13039/501100000780 H2020 760331 |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reconocimiento (by) http://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 Reconocimiento (by) http://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
MDPI |
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MDPI |
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reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname:Universitat Politècnica de València (UPV) |
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RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
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