Electrocatalytic tuning of biosensing response through electrostatic or hydrophobic enzyme-graphene oxide interactions

The effect of graphene oxidative grades upon the conductivity and hydrophobicity and consequently the influence on an enzymatic biosensing response is presented. The electrochemical responses of reduced graphene oxide (rGO) have been compared with the responses obtained from the oxide form (oGO) and...

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Autores: Baptista Pires, Luis Miguel|||0000-0002-2290-6386, Pérez-López, Briza, Mayorga-Martinez, Carmen C.|||0000-0003-3687-0035, Morales-Narváez, Eden|||0000-0002-1536-825X, Domingo Marimon, Neus|||0000-0002-5229-6638, Esplandiu Egido, Maria José|||0000-0003-2079-0639, Alzina, Francesc|||0000-0002-7082-0624, Sotomayor Torres, Clivia M.|||0000-0001-9986-2716, Merkoçi, Arben|||0000-0003-2486-8085
Tipo de documento: artigo
Data de publicação:2014
País:España
Recursos:Universitat Autònoma de Barcelona
Repositório:Dipòsit Digital de Documents de la UAB
Idioma:inglês
OAI Identifier:oai:ddd.uab.cat:282554
Acesso em linha:https://ddd.uab.cat/record/282554
https://dx.doi.org/urn:doi:10.1016/j.bios.2014.05.028
Access Level:Acceso aberto
Palavra-chave:Oxidized graphene oxide
Reduced graphene oxide
Electrostatic interactions
Hydrophobic interactions
Electrocatalytic tune
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spelling Electrocatalytic tuning of biosensing response through electrostatic or hydrophobic enzyme-graphene oxide interactionsBaptista Pires, Luis Miguel|||0000-0002-2290-6386Pérez-López, BrizaMayorga-Martinez, Carmen C.|||0000-0003-3687-0035Morales-Narváez, Eden|||0000-0002-1536-825XDomingo Marimon, Neus|||0000-0002-5229-6638Esplandiu Egido, Maria José|||0000-0003-2079-0639Alzina, Francesc|||0000-0002-7082-0624Sotomayor Torres, Clivia M.|||0000-0001-9986-2716Merkoçi, Arben|||0000-0003-2486-8085Oxidized graphene oxideReduced graphene oxideElectrostatic interactionsHydrophobic interactionsElectrocatalytic tuneThe effect of graphene oxidative grades upon the conductivity and hydrophobicity and consequently the influence on an enzymatic biosensing response is presented. The electrochemical responses of reduced graphene oxide (rGO) have been compared with the responses obtained from the oxide form (oGO) and their performances have been accordingly discussed with various evidences obtained by optical techniques. We used tyrosinase enzyme as a proof of concept receptor with interest for phenolic compounds detection through its direct adsorption onto a screen-printed carbon electrode previously modified with oGO or rGO with a carbon-oxygen ratio of 1.07 or 1.53 respectively. Different levels of oGO directly affect the (bio)conjugation properties of the biosensor due to changes at enzyme/graphene oxide interface coming from the various electrostatic or hydrophobic interactions with biomolecules. The developed biosensor was capable of reaching a limit of detection of 0.01. nM catechol. This tuning capability of the biosensor response can be of interest for building several other biosensors, including immunosensors and DNA sensors for various applications 22014-01-0120142014-01-01Articlehttp://purl.org/coar/resource_type/c_6501SMURhttp://purl.org/coar/version/c_71e4c1898caa6e32info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/282554https://dx.doi.org/urn:doi:10.1016/j.bios.2014.05.028reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Ciencia e Innovación https://doi.org/10.13039/501100004837 MAT2011-25870open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2825542026-06-06T12:50:31Z
dc.title.none.fl_str_mv Electrocatalytic tuning of biosensing response through electrostatic or hydrophobic enzyme-graphene oxide interactions
title Electrocatalytic tuning of biosensing response through electrostatic or hydrophobic enzyme-graphene oxide interactions
spellingShingle Electrocatalytic tuning of biosensing response through electrostatic or hydrophobic enzyme-graphene oxide interactions
Baptista Pires, Luis Miguel|||0000-0002-2290-6386
Oxidized graphene oxide
Reduced graphene oxide
Electrostatic interactions
Hydrophobic interactions
Electrocatalytic tune
title_short Electrocatalytic tuning of biosensing response through electrostatic or hydrophobic enzyme-graphene oxide interactions
title_full Electrocatalytic tuning of biosensing response through electrostatic or hydrophobic enzyme-graphene oxide interactions
title_fullStr Electrocatalytic tuning of biosensing response through electrostatic or hydrophobic enzyme-graphene oxide interactions
title_full_unstemmed Electrocatalytic tuning of biosensing response through electrostatic or hydrophobic enzyme-graphene oxide interactions
title_sort Electrocatalytic tuning of biosensing response through electrostatic or hydrophobic enzyme-graphene oxide interactions
dc.creator.none.fl_str_mv Baptista Pires, Luis Miguel|||0000-0002-2290-6386
Pérez-López, Briza
Mayorga-Martinez, Carmen C.|||0000-0003-3687-0035
Morales-Narváez, Eden|||0000-0002-1536-825X
Domingo Marimon, Neus|||0000-0002-5229-6638
Esplandiu Egido, Maria José|||0000-0003-2079-0639
Alzina, Francesc|||0000-0002-7082-0624
Sotomayor Torres, Clivia M.|||0000-0001-9986-2716
Merkoçi, Arben|||0000-0003-2486-8085
author Baptista Pires, Luis Miguel|||0000-0002-2290-6386
author_facet Baptista Pires, Luis Miguel|||0000-0002-2290-6386
Pérez-López, Briza
Mayorga-Martinez, Carmen C.|||0000-0003-3687-0035
Morales-Narváez, Eden|||0000-0002-1536-825X
Domingo Marimon, Neus|||0000-0002-5229-6638
Esplandiu Egido, Maria José|||0000-0003-2079-0639
Alzina, Francesc|||0000-0002-7082-0624
Sotomayor Torres, Clivia M.|||0000-0001-9986-2716
Merkoçi, Arben|||0000-0003-2486-8085
author_role author
author2 Pérez-López, Briza
Mayorga-Martinez, Carmen C.|||0000-0003-3687-0035
Morales-Narváez, Eden|||0000-0002-1536-825X
Domingo Marimon, Neus|||0000-0002-5229-6638
Esplandiu Egido, Maria José|||0000-0003-2079-0639
Alzina, Francesc|||0000-0002-7082-0624
Sotomayor Torres, Clivia M.|||0000-0001-9986-2716
Merkoçi, Arben|||0000-0003-2486-8085
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Oxidized graphene oxide
Reduced graphene oxide
Electrostatic interactions
Hydrophobic interactions
Electrocatalytic tune
topic Oxidized graphene oxide
Reduced graphene oxide
Electrostatic interactions
Hydrophobic interactions
Electrocatalytic tune
description The effect of graphene oxidative grades upon the conductivity and hydrophobicity and consequently the influence on an enzymatic biosensing response is presented. The electrochemical responses of reduced graphene oxide (rGO) have been compared with the responses obtained from the oxide form (oGO) and their performances have been accordingly discussed with various evidences obtained by optical techniques. We used tyrosinase enzyme as a proof of concept receptor with interest for phenolic compounds detection through its direct adsorption onto a screen-printed carbon electrode previously modified with oGO or rGO with a carbon-oxygen ratio of 1.07 or 1.53 respectively. Different levels of oGO directly affect the (bio)conjugation properties of the biosensor due to changes at enzyme/graphene oxide interface coming from the various electrostatic or hydrophobic interactions with biomolecules. The developed biosensor was capable of reaching a limit of detection of 0.01. nM catechol. This tuning capability of the biosensor response can be of interest for building several other biosensors, including immunosensors and DNA sensors for various applications
publishDate 2014
dc.date.none.fl_str_mv 2
2014-01-01
2014
2014-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
SMUR
http://purl.org/coar/version/c_71e4c1898caa6e32
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/282554
https://dx.doi.org/urn:doi:10.1016/j.bios.2014.05.028
url https://ddd.uab.cat/record/282554
https://dx.doi.org/urn:doi:10.1016/j.bios.2014.05.028
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 MAT2011-25870
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301629

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