Electroresponsive hydrogel for wound healing with real-time monitoring of bacterial NADH to prevent infection
The work leading to this publication was supported by the Feodor Lynen Research Fellowship of the Alexander von Humboldt Foundation. This work made use of the resources of the Correlative Microscopy and Tomography (CoMiTo) core facility at Saarland University. Funding for the femtosecond laser by De...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2026 |
| País: | España |
| Institución: | Universitat Politècnica de Catalunya (UPC) |
| Repositorio: | UPCommons. Portal del coneixement obert de la UPC |
| Idioma: | inglés |
| OAI Identifier: | oai:dnet:upcommonspor::00ac1c1b6744f0a1e1a346139947a206 |
| Acceso en línea: | https://hdl.handle.net/2117/459956 https://dx.doi.org/10.1021/acsapm.5c04585 |
| Access Level: | acceso abierto |
| Palabra clave: | Hyaluronic acid Click chemistry PEDOT Conducting polymer Molecular dynamics Computer simulation |
| Sumario: | The work leading to this publication was supported by the Feodor Lynen Research Fellowship of the Alexander von Humboldt Foundation. This work made use of the resources of the Correlative Microscopy and Tomography (CoMiTo) core facility at Saarland University. Funding for the femtosecond laser by Deutsche Forschungsgemeinschaft (DFG Project No. 467354208) is gratefully acknowledged. The authors thank Saar-Hartmetall und Werkzeuge GmbH for providing the materials used in this study.Stimuli-responsive hydrogels represent an important class of materials for biomedical applications. Electroresponsive hyaluronic acid (HA) hydrogels synthesized via a click-reaction between thiol-functionalized HA and semi-interpenetrated with poly(hydroxymethyl-3,4-ethylenedioxythiophene) (PEDOT-MeOH), which are denoted as clickHA/PEDOT-MeOH, were found to promote cell migration upon electrostimulation and to exhibit very promising wound healing activity. In this work, we focus on the electrochemical response of clickHA/PEDOT-MeOH hydrogels by analyzing the electrochemical stability, the internal structure of the semi-interpenetrated material, and the capacity to act as electrochemical sensors. After demonstrating the remarkable electrochemical stability of clickHA/PEDOT-MeOH using cyclic voltammetry, the influence of the semi-interpenetrated PEDOT-MeOH chains on the structure of the hydrogel matrix and the interactions connecting both components are studied using molecular dynamics (MD) simulations. The results reveal that the accommodation of PEDOT-MeOH chains after the polymerization of the EDOT-MeOH monomers caused only minor modifications in the pore architecture. Finally, the selective electrochemical detection of nicotinamide adenine dinucleotide (NADH), a biomarker used to identify bacterial infection, was demonstrated both in laboratory-prepared solutions and in vitro using bacterial culture media. Overall, our findings indicate that the semi-interpenetrated clickHA/PEDOT-MeOH hydrogel behaves as a bifunctional platform capable of simultaneously promoting tissue repair and selectively detecting NADH, thereby enabling the prevention of bacterial infections during the healing process. |
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