Magnetic multiwalled carbon nanotubes as nanocarrier tags for sensitive determination of fetuin in saliva

This paper reports the development and performance of an electrochemical immunosensor using magnetic multiwalled carbon nanotubes (m-MWCNTs) as nanocarrier tags for the determination of human fetuin A (HFA), a relevant biomarker of obesity, insulin resistance, and type-2 diabetes as well as for panc...

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Detalles Bibliográficos
Autores: Sánchez Tirado, E, González Cortés, Araceli, Yáñez-Sedeño Orive, Paloma, Pingarrón Carrazón, José Manuel
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/18772
Acceso en línea:https://hdl.handle.net/20.500.14352/18772
Access Level:acceso abierto
Palabra clave:543
Electrochemical immunosensor
Fetuin
Magnetic carbon nanotubes
Saliva
Química analítica (Química)
2301 Química Analítica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/18772
network_acronym_str ES
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repository_id_str
spelling Magnetic multiwalled carbon nanotubes as nanocarrier tags for sensitive determination of fetuin in salivaSánchez Tirado, EGonzález Cortés, AraceliYáñez-Sedeño Orive, PalomaPingarrón Carrazón, José Manuel543Electrochemical immunosensorFetuinMagnetic carbon nanotubesSalivaQuímica analítica (Química)2301 Química AnalíticaThis paper reports the development and performance of an electrochemical immunosensor using magnetic multiwalled carbon nanotubes (m-MWCNTs) as nanocarrier tags for the determination of human fetuin A (HFA), a relevant biomarker of obesity, insulin resistance, and type-2 diabetes as well as for pancreatic and liver cancers and inflammatory processes. Screen-printed carbon electrodes were grafted with p-aminobezoic acid and streptavidin was covalently immobilized on the electrode surface. A biotinylated capture antibody was immobilized through streptavidin-biotin interaction and a sandwich assay configuration was implemented using m-MWCNTs conjugated with HRP and anti-HFA antibodies as the detection label. The determination of HFA was accomplished by measuring the current produced by the electrochemical reduction of benzoquinone at -200 mV upon addition of H2O2 as HRP substrate. The prepared m-MWCNTs were characterized by SEM, TEM, XRD and EDS. All the steps involved in the immunosensor preparation were monitored by electrochemical impedance spectroscopy and cyclic voltammetry. A linear calibration plot for HFA was found between 20 and 2000 pg/mL with a LOD value of 16 pg/mL. This performance is notably better than that reported for an ELISA kit and a chronoimpedimetric immunosensor. The favorable contribution of m-MWCNTs in comparison with MWCNTs without incorporated magnetic particles to this excellent analytical performance is also highlighted. The immunosensor selectivity against other proteins and potentially interfering compounds was excellent. In addition, the usefulness of the immunosensor was demonstrated by the analysis of HFA in saliva with minimal sample treatment.ElsevierUniversidad Complutense de Madrid20182018-01-0120182018-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/18772reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/187722026-06-02T12:44:21Z
dc.title.none.fl_str_mv Magnetic multiwalled carbon nanotubes as nanocarrier tags for sensitive determination of fetuin in saliva
title Magnetic multiwalled carbon nanotubes as nanocarrier tags for sensitive determination of fetuin in saliva
spellingShingle Magnetic multiwalled carbon nanotubes as nanocarrier tags for sensitive determination of fetuin in saliva
Sánchez Tirado, E
543
Electrochemical immunosensor
Fetuin
Magnetic carbon nanotubes
Saliva
Química analítica (Química)
2301 Química Analítica
title_short Magnetic multiwalled carbon nanotubes as nanocarrier tags for sensitive determination of fetuin in saliva
title_full Magnetic multiwalled carbon nanotubes as nanocarrier tags for sensitive determination of fetuin in saliva
title_fullStr Magnetic multiwalled carbon nanotubes as nanocarrier tags for sensitive determination of fetuin in saliva
title_full_unstemmed Magnetic multiwalled carbon nanotubes as nanocarrier tags for sensitive determination of fetuin in saliva
title_sort Magnetic multiwalled carbon nanotubes as nanocarrier tags for sensitive determination of fetuin in saliva
dc.creator.none.fl_str_mv Sánchez Tirado, E
González Cortés, Araceli
Yáñez-Sedeño Orive, Paloma
Pingarrón Carrazón, José Manuel
author Sánchez Tirado, E
author_facet Sánchez Tirado, E
González Cortés, Araceli
Yáñez-Sedeño Orive, Paloma
Pingarrón Carrazón, José Manuel
author_role author
author2 González Cortés, Araceli
Yáñez-Sedeño Orive, Paloma
Pingarrón Carrazón, José Manuel
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 543
Electrochemical immunosensor
Fetuin
Magnetic carbon nanotubes
Saliva
Química analítica (Química)
2301 Química Analítica
topic 543
Electrochemical immunosensor
Fetuin
Magnetic carbon nanotubes
Saliva
Química analítica (Química)
2301 Química Analítica
description This paper reports the development and performance of an electrochemical immunosensor using magnetic multiwalled carbon nanotubes (m-MWCNTs) as nanocarrier tags for the determination of human fetuin A (HFA), a relevant biomarker of obesity, insulin resistance, and type-2 diabetes as well as for pancreatic and liver cancers and inflammatory processes. Screen-printed carbon electrodes were grafted with p-aminobezoic acid and streptavidin was covalently immobilized on the electrode surface. A biotinylated capture antibody was immobilized through streptavidin-biotin interaction and a sandwich assay configuration was implemented using m-MWCNTs conjugated with HRP and anti-HFA antibodies as the detection label. The determination of HFA was accomplished by measuring the current produced by the electrochemical reduction of benzoquinone at -200 mV upon addition of H2O2 as HRP substrate. The prepared m-MWCNTs were characterized by SEM, TEM, XRD and EDS. All the steps involved in the immunosensor preparation were monitored by electrochemical impedance spectroscopy and cyclic voltammetry. A linear calibration plot for HFA was found between 20 and 2000 pg/mL with a LOD value of 16 pg/mL. This performance is notably better than that reported for an ELISA kit and a chronoimpedimetric immunosensor. The favorable contribution of m-MWCNTs in comparison with MWCNTs without incorporated magnetic particles to this excellent analytical performance is also highlighted. The immunosensor selectivity against other proteins and potentially interfering compounds was excellent. In addition, the usefulness of the immunosensor was demonstrated by the analysis of HFA in saliva with minimal sample treatment.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01
2018
2018-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/18772
url https://hdl.handle.net/20.500.14352/18772
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300724

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