Viologen-functionalized single-walled carbon nanotubes as carrier nanotags for electrochemical immunosensing. Application to TGF-β1 cytokine
Viologen-SWCNT hybrids are synthesized by aryl-diazonium chemistry in the presence of isoamyl nitrite followed by condensation reaction of the resulting HOOC-PheSWCNT with 1-(3-aminoethyl)-4,4’-bipyridinium bromine and N-alkylation with 2- bromoethylamine. The V-Phe-SWCNT hybrids were characterized...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2017 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/18188 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/18188 |
| Access Level: | acceso abierto |
| Palabra clave: | 543 Viologen Transforming growth factor β1 Cytokine Saliva Electrochemical immunosensor Química analítica (Química) 2301 Química Analítica |
| Sumario: | Viologen-SWCNT hybrids are synthesized by aryl-diazonium chemistry in the presence of isoamyl nitrite followed by condensation reaction of the resulting HOOC-PheSWCNT with 1-(3-aminoethyl)-4,4’-bipyridinium bromine and N-alkylation with 2- bromoethylamine. The V-Phe-SWCNT hybrids were characterized by using different spectroscopic techniques (FT-IR, Raman, UV-vis), TGA and Kaiser test. ViologenSWCNTs were used for the preparation of an electrochemical immunosensor for the determination of the transforming growth factor β1 (TGF-β1) cytokine considered as a reliable biomarker in several human diseases. The methodology involved preparation of VPhe-SWCNT(-HRP)-anti-TGF conjugates by covalent linkage of HRP and anti-TGF onto V-Phe-SWCNT hybrids. Biotinylated anti-TGF antibodies were immobilized onto 4- carboxyphenyl-functionalized SPCEs modified with streptavidin and a sandwich type 2 immunoassay was implemented for TGF-β1 with signal amplification using V-PheSWCNT(-HRP)-anti-TGF conjugates as carrier tags. The analytical characteristics exhibited by the as prepared immunosensor (range of linearity between 2.5 and 1000 pg mL-1 TGF-β1; detection limit of 0.95 pg mL-1 ) improve notably those reported with other previous immunosensors or ELISA kits. A great selectivity against other proteins was also found. The prepared immunosensor was validated by determining TGF-β1 in real saliva samples. Minimal sample treatment was required and the obtained results were in excellent agreement with those obtained by using a commercial ELISA kit. |
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