Porcine circovirus 3 is highly prevalent in serum and tissues and may persistently infect wild boar (Sus scrofa scrofa)
Porcine circovirus 3 (PCV‐3) prevalence has been minimally investigated in wild boar; dynamics of infection and viral tissue distribution are currently unknown. In this study, serum samples from 518 wild boar (from years 2004 to 2018) were used to study frequency of infection. Also, serum samples fr...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:10459.1/67843 |
| Acceso en línea: | https://doi.org/10.1111/tbed.12988 http://hdl.handle.net/10459.1/67843 |
| Access Level: | acceso abierto |
| Palabra clave: | Infection dynamics Wild boar Tissue distribution Porcine circovirus 3 |
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Porcine circovirus 3 is highly prevalent in serum and tissues and may persistently infect wild boar (Sus scrofa scrofa)Klaumann, FranciniDias-Alves, AndreaCabezón Ponsoda, ÓscarMentaberre García, GregorioCastillo-Contreras, RaquelLópez-Béjar, M.Casas-Díaz, EncarnaSibila, MarinaCorrea Fiz, FlorenciaSegalés, JoaquimInfection dynamicsWild boarTissue distributionPorcine circovirus 3Porcine circovirus 3 (PCV‐3) prevalence has been minimally investigated in wild boar; dynamics of infection and viral tissue distribution are currently unknown. In this study, serum samples from 518 wild boar (from years 2004 to 2018) were used to study frequency of infection. Also, serum samples from 19 boar captured and recaptured at least two times for a period of time from 1 month to 1 year were collected to determine PCV‐3 infection dynamics. Finally, to elucidate PCV‐3 DNA organic distribution, sera, different tissues and faeces were obtained from 35 additional wild boar. PCV‐3 DNA was extracted and amplified with a conventional PCR. For the PCV‐3 PCR‐positive sera from the longitudinally sampled and different tissue types, a quantitative PCR was performed. Genome sequence was obtained from a number of PCV‐3 PCR‐positive samples from different years, different time‐points of infection and tissues. Obtained results confirmed the susceptibility of wild boar to the virus, showing high frequency of PCV‐3 detection (221 out of 518, 42.66%) and demonstrating circulation at least since 2004. Compiled data indicate the possibility of long‐term infections, since 5 out of 10 PCV‐3 PCR‐positive boars longitudinally sampled showed positivity in samplings separated for more than 5 months. All tested tissue types' harboured PCV‐3 genome, with the highest percentage of PCR positivity in submandibular lymph node, tonsil, lung, liver, spleen and kidney. The amount of DNA in all tested PCV‐3 PCR‐positive samples was moderate to low. All partial and complete PCV‐3 sequences obtained from wild boar displayed high nucleotide identity, higher than 98%. In conclusion, this study further confirms that wild boar is susceptible to PCV‐3 infection, showing high frequency of detection in this animal species. Furthermore, PCV‐3 can be found in different tissues of wild boar and is apparently able to cause persistent infection.Instituto Nacional de Investigación y Tecnologia Agraria y Alimentaria. Grant Number: E‐RTA2017‐00007‐00‐00Blackwell Verlag GmbH2020202020182020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.1111/tbed.12988http://hdl.handle.net/10459.1/67843http://hdl.handle.net/10459.1/67843reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1111/tbed.12988Transboundary And Emerging Diseases, 2018, vol. 66, num. 1, p. 91-101cc-by-nc (c) Klaumann, Francini et al., 2018info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/4.0/oai:recercat.cat:10459.1/678432026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Porcine circovirus 3 is highly prevalent in serum and tissues and may persistently infect wild boar (Sus scrofa scrofa) |
| title |
Porcine circovirus 3 is highly prevalent in serum and tissues and may persistently infect wild boar (Sus scrofa scrofa) |
| spellingShingle |
Porcine circovirus 3 is highly prevalent in serum and tissues and may persistently infect wild boar (Sus scrofa scrofa) Klaumann, Francini Infection dynamics Wild boar Tissue distribution Porcine circovirus 3 |
| title_short |
Porcine circovirus 3 is highly prevalent in serum and tissues and may persistently infect wild boar (Sus scrofa scrofa) |
| title_full |
Porcine circovirus 3 is highly prevalent in serum and tissues and may persistently infect wild boar (Sus scrofa scrofa) |
| title_fullStr |
Porcine circovirus 3 is highly prevalent in serum and tissues and may persistently infect wild boar (Sus scrofa scrofa) |
| title_full_unstemmed |
Porcine circovirus 3 is highly prevalent in serum and tissues and may persistently infect wild boar (Sus scrofa scrofa) |
| title_sort |
Porcine circovirus 3 is highly prevalent in serum and tissues and may persistently infect wild boar (Sus scrofa scrofa) |
| dc.creator.none.fl_str_mv |
Klaumann, Francini Dias-Alves, Andrea Cabezón Ponsoda, Óscar Mentaberre García, Gregorio Castillo-Contreras, Raquel López-Béjar, M. Casas-Díaz, Encarna Sibila, Marina Correa Fiz, Florencia Segalés, Joaquim |
| author |
Klaumann, Francini |
| author_facet |
Klaumann, Francini Dias-Alves, Andrea Cabezón Ponsoda, Óscar Mentaberre García, Gregorio Castillo-Contreras, Raquel López-Béjar, M. Casas-Díaz, Encarna Sibila, Marina Correa Fiz, Florencia Segalés, Joaquim |
| author_role |
author |
| author2 |
Dias-Alves, Andrea Cabezón Ponsoda, Óscar Mentaberre García, Gregorio Castillo-Contreras, Raquel López-Béjar, M. Casas-Díaz, Encarna Sibila, Marina Correa Fiz, Florencia Segalés, Joaquim |
| author2_role |
author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Infection dynamics Wild boar Tissue distribution Porcine circovirus 3 |
| topic |
Infection dynamics Wild boar Tissue distribution Porcine circovirus 3 |
| description |
Porcine circovirus 3 (PCV‐3) prevalence has been minimally investigated in wild boar; dynamics of infection and viral tissue distribution are currently unknown. In this study, serum samples from 518 wild boar (from years 2004 to 2018) were used to study frequency of infection. Also, serum samples from 19 boar captured and recaptured at least two times for a period of time from 1 month to 1 year were collected to determine PCV‐3 infection dynamics. Finally, to elucidate PCV‐3 DNA organic distribution, sera, different tissues and faeces were obtained from 35 additional wild boar. PCV‐3 DNA was extracted and amplified with a conventional PCR. For the PCV‐3 PCR‐positive sera from the longitudinally sampled and different tissue types, a quantitative PCR was performed. Genome sequence was obtained from a number of PCV‐3 PCR‐positive samples from different years, different time‐points of infection and tissues. Obtained results confirmed the susceptibility of wild boar to the virus, showing high frequency of PCV‐3 detection (221 out of 518, 42.66%) and demonstrating circulation at least since 2004. Compiled data indicate the possibility of long‐term infections, since 5 out of 10 PCV‐3 PCR‐positive boars longitudinally sampled showed positivity in samplings separated for more than 5 months. All tested tissue types' harboured PCV‐3 genome, with the highest percentage of PCR positivity in submandibular lymph node, tonsil, lung, liver, spleen and kidney. The amount of DNA in all tested PCV‐3 PCR‐positive samples was moderate to low. All partial and complete PCV‐3 sequences obtained from wild boar displayed high nucleotide identity, higher than 98%. In conclusion, this study further confirms that wild boar is susceptible to PCV‐3 infection, showing high frequency of detection in this animal species. Furthermore, PCV‐3 can be found in different tissues of wild boar and is apparently able to cause persistent infection. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018 2020 2020 2020 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://doi.org/10.1111/tbed.12988 http://hdl.handle.net/10459.1/67843 http://hdl.handle.net/10459.1/67843 |
| url |
https://doi.org/10.1111/tbed.12988 http://hdl.handle.net/10459.1/67843 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Reproducció del document publicat a: https://doi.org/10.1111/tbed.12988 Transboundary And Emerging Diseases, 2018, vol. 66, num. 1, p. 91-101 |
| dc.rights.none.fl_str_mv |
cc-by-nc (c) Klaumann, Francini et al., 2018 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc/4.0/ |
| rights_invalid_str_mv |
cc-by-nc (c) Klaumann, Francini et al., 2018 https://creativecommons.org/licenses/by-nc/4.0/ |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Blackwell Verlag GmbH |
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Blackwell Verlag GmbH |
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reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Recercat. Dipósit de la Recerca de Catalunya |
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Recercat. Dipósit de la Recerca de Catalunya |
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