Porcine circovirus 3 is highly prevalent in serum and tissues and may persistently infect wild boar (Sus scrofa scrofa)
Porcine circovirus 3 (-3) prevalence has been minimally investigated in wild boar; dynamics of infection and viral tissue distribution are currently unknown. In this study, serum samples from 518 wild boar (from years 2004 to 2018) were used to study frequency of infection. Also, serum samples from...
| Autores: | , , , , , , , , , |
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| Tipo de documento: | artigo |
| Data de publicação: | 2018 |
| País: | España |
| Recursos: | Universitat Autònoma de Barcelona |
| Repositório: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglês |
| OAI Identifier: | oai:ddd.uab.cat:227983 |
| Acesso em linha: | https://ddd.uab.cat/record/227983 https://dx.doi.org/urn:doi:10.1111/tbed.12988 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Infection dynamics Porcine circovirus 3 Prevalence Retrospective Tissue distribution Wild boar |
| Resumo: | Porcine circovirus 3 (-3) prevalence has been minimally investigated in wild boar; dynamics of infection and viral tissue distribution are currently unknown. In this study, serum samples from 518 wild boar (from years 2004 to 2018) were used to study frequency of infection. Also, serum samples from 19 boar captured and recaptured at least two times for a period of time from 1 month to 1 year were collected to determine -3 infection dynamics. Finally, to elucidate -3 organic distribution, sera, different tissues and faeces were obtained from 35 additional wild boar. -3 was extracted and amplified with a conventional . For the -3 -positive sera from the longitudinally sampled and different tissue types, a quantitative was performed. Genome sequence was obtained from a number of -3 -positive samples from different years, different time-points of infection and tissues. Obtained results confirmed the susceptibility of wild boar to the virus, showing high frequency of -3 detection (221 out of 518, 42.66%) and demonstrating circulation at least since 2004. Compiled data indicate the possibility of long-term infections, since 5 out of 10 -3 -positive boars longitudinally sampled showed positivity in samplings separated for more than 5 months. All tested tissue types' harboured -3 genome, with the highest percentage of positivity in submandibular lymph node, tonsil, lung, liver, spleen and kidney. The amount of in all tested -3 -positive samples was moderate to low. All partial and complete -3 sequences obtained from wild boar displayed high nucleotide identity, higher than 98%. In conclusion, this study further confirms that wild boar is susceptible to -3 infection, showing high frequency of detection in this animal species. Furthermore, -3 can be found in different tissues of wild boar and is apparently able to cause persistent infection. |
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