Experimental study of the evanescent-wave photonic sensors response in presence of molecular beacon conformational changes
[EN] An experimental study of the influence of the conformational change suffered by molecular beacon (MB) probesupon the biorecognition of nucleic acid target oligonucleotides over evanescent wave photonic sensorsis reported. To this end, high sensitivity photonic sensors based on silicon photonic...
| Autores: | , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/136756 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/136756 |
| Access Level: | acceso abierto |
| Palabra clave: | Conformational change Molecular beacon Photonic bandgap Photonic biosensor Silicon photonics QUIMICA ANALITICA TEORIA DE LA SEÑAL Y COMUNICACIONES |
| id |
ES_cd6941ea2aa402639a5bc26cec6e7ebc |
|---|---|
| oai_identifier_str |
oai:riunet.upv.es:10251/136756 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
Experimental study of the evanescent-wave photonic sensors response in presence of molecular beacon conformational changesRuiz-Tórtola, ÁngelaPrats-Quílez, FranciscoGonzalez-Lucas, DanielWheeler, GuyDalmay, TamasBohlmann, HelgeGötzen, ReinerBañuls Polo, María-José|||0000-0002-2422-7731Maquieira, Angel|||0000-0003-4641-4957Griol Barres, AmadeuHurtado Montañés, Juan|||0000-0002-4870-7641García-Rupérez, Jaime|||0000-0002-2036-0084Conformational changeMolecular beaconPhotonic bandgapPhotonic biosensorSilicon photonicsQUIMICA ANALITICATEORIA DE LA SEÑAL Y COMUNICACIONES[EN] An experimental study of the influence of the conformational change suffered by molecular beacon (MB) probesupon the biorecognition of nucleic acid target oligonucleotides over evanescent wave photonic sensorsis reported. To this end, high sensitivity photonic sensors based on silicon photonic bandgap (PBG) structures were used, where the MB probes were immobilized via their 5 termination. Those MBs incorporate a biotin moiety close to their 3 termination in order to selectively bind a streptavidin molecule to them. The different photonic sensing responses obtained toward the target oligonucleotide detection, when the streptavidin molecule was bound to the MB probes or not, demonstrate the conformational change suffered by the MB upon hybridization, which promotes the displacement of the streptavidin molecule away from the surface of the photonic sensing structure.Horizon 2020 Framework Programme, Grant/Award Number: ICT-644242; MINECO, Grant/Award Number: CTQ/2016/75749-RJohn Wiley & SonsEscuela Técnica Superior de Ingeniería de TelecomunicaciónDepartamento de ComunicacionesDepartamento de QuímicaInstituto Universitario de Tecnología NanofotónicaInstituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo TecnológicoEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalEuropean CommissionMinisterio de Economía y CompetitividadRepositorio Institucional de la Universitat Politècnica de València Riunet20182018-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/136756reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengEuropean Commission https://doi.org/10.13039/501100000780 H2020 644242 Self-amplified photonic biosensing platform for microRNA-based early diagnosis of diseasesMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 CTQ2016-75749-R BIOSENSORES HOLOGRAFICOS. PRUEBA DE CONCEPTO Y DEMOSTRACION EN APLICACIONES CLINICASopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1367562026-06-13T07:49:27Z |
| dc.title.none.fl_str_mv |
Experimental study of the evanescent-wave photonic sensors response in presence of molecular beacon conformational changes |
| title |
Experimental study of the evanescent-wave photonic sensors response in presence of molecular beacon conformational changes |
| spellingShingle |
Experimental study of the evanescent-wave photonic sensors response in presence of molecular beacon conformational changes Ruiz-Tórtola, Ángela Conformational change Molecular beacon Photonic bandgap Photonic biosensor Silicon photonics QUIMICA ANALITICA TEORIA DE LA SEÑAL Y COMUNICACIONES |
| title_short |
Experimental study of the evanescent-wave photonic sensors response in presence of molecular beacon conformational changes |
| title_full |
Experimental study of the evanescent-wave photonic sensors response in presence of molecular beacon conformational changes |
| title_fullStr |
Experimental study of the evanescent-wave photonic sensors response in presence of molecular beacon conformational changes |
| title_full_unstemmed |
Experimental study of the evanescent-wave photonic sensors response in presence of molecular beacon conformational changes |
| title_sort |
Experimental study of the evanescent-wave photonic sensors response in presence of molecular beacon conformational changes |
| dc.creator.none.fl_str_mv |
Ruiz-Tórtola, Ángela Prats-Quílez, Francisco Gonzalez-Lucas, Daniel Wheeler, Guy Dalmay, Tamas Bohlmann, Helge Götzen, Reiner Bañuls Polo, María-José|||0000-0002-2422-7731 Maquieira, Angel|||0000-0003-4641-4957 Griol Barres, Amadeu Hurtado Montañés, Juan|||0000-0002-4870-7641 García-Rupérez, Jaime|||0000-0002-2036-0084 |
| author |
Ruiz-Tórtola, Ángela |
| author_facet |
Ruiz-Tórtola, Ángela Prats-Quílez, Francisco Gonzalez-Lucas, Daniel Wheeler, Guy Dalmay, Tamas Bohlmann, Helge Götzen, Reiner Bañuls Polo, María-José|||0000-0002-2422-7731 Maquieira, Angel|||0000-0003-4641-4957 Griol Barres, Amadeu Hurtado Montañés, Juan|||0000-0002-4870-7641 García-Rupérez, Jaime|||0000-0002-2036-0084 |
| author_role |
author |
| author2 |
Prats-Quílez, Francisco Gonzalez-Lucas, Daniel Wheeler, Guy Dalmay, Tamas Bohlmann, Helge Götzen, Reiner Bañuls Polo, María-José|||0000-0002-2422-7731 Maquieira, Angel|||0000-0003-4641-4957 Griol Barres, Amadeu Hurtado Montañés, Juan|||0000-0002-4870-7641 García-Rupérez, Jaime|||0000-0002-2036-0084 |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Escuela Técnica Superior de Ingeniería de Telecomunicación Departamento de Comunicaciones Departamento de Química Instituto Universitario de Tecnología Nanofotónica Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural European Commission Ministerio de Economía y Competitividad Repositorio Institucional de la Universitat Politècnica de València Riunet |
| dc.subject.none.fl_str_mv |
Conformational change Molecular beacon Photonic bandgap Photonic biosensor Silicon photonics QUIMICA ANALITICA TEORIA DE LA SEÑAL Y COMUNICACIONES |
| topic |
Conformational change Molecular beacon Photonic bandgap Photonic biosensor Silicon photonics QUIMICA ANALITICA TEORIA DE LA SEÑAL Y COMUNICACIONES |
| description |
[EN] An experimental study of the influence of the conformational change suffered by molecular beacon (MB) probesupon the biorecognition of nucleic acid target oligonucleotides over evanescent wave photonic sensorsis reported. To this end, high sensitivity photonic sensors based on silicon photonic bandgap (PBG) structures were used, where the MB probes were immobilized via their 5 termination. Those MBs incorporate a biotin moiety close to their 3 termination in order to selectively bind a streptavidin molecule to them. The different photonic sensing responses obtained toward the target oligonucleotide detection, when the streptavidin molecule was bound to the MB probes or not, demonstrate the conformational change suffered by the MB upon hybridization, which promotes the displacement of the streptavidin molecule away from the surface of the photonic sensing structure. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018 2018-01-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://riunet.upv.es/handle/10251/136756 |
| url |
https://riunet.upv.es/handle/10251/136756 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
European Commission https://doi.org/10.13039/501100000780 H2020 644242 Self-amplified photonic biosensing platform for microRNA-based early diagnosis of diseases Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 CTQ2016-75749-R BIOSENSORES HOLOGRAFICOS. PRUEBA DE CONCEPTO Y DEMOSTRACION EN APLICACIONES CLINICAS |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reconocimiento (by) http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reconocimiento (by) http://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
John Wiley & Sons |
| publisher.none.fl_str_mv |
John Wiley & Sons |
| dc.source.none.fl_str_mv |
reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname:Universitat Politècnica de València (UPV) |
| instname_str |
Universitat Politècnica de València (UPV) |
| reponame_str |
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| collection |
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869419830530015232 |
| score |
15.300719 |