Experimental study of the evanescent-wave photonic sensors response in presence of molecular beacon conformational changes
[EN] An experimental study of the influence of the conformational change suffered by molecular beacon (MB) probesupon the biorecognition of nucleic acid target oligonucleotides over evanescent wave photonic sensorsis reported. To this end, high sensitivity photonic sensors based on silicon photonic...
| Autores: | , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/136756 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/136756 |
| Access Level: | acceso abierto |
| Palabra clave: | Conformational change Molecular beacon Photonic bandgap Photonic biosensor Silicon photonics QUIMICA ANALITICA TEORIA DE LA SEÑAL Y COMUNICACIONES |
| Sumario: | [EN] An experimental study of the influence of the conformational change suffered by molecular beacon (MB) probesupon the biorecognition of nucleic acid target oligonucleotides over evanescent wave photonic sensorsis reported. To this end, high sensitivity photonic sensors based on silicon photonic bandgap (PBG) structures were used, where the MB probes were immobilized via their 5 termination. Those MBs incorporate a biotin moiety close to their 3 termination in order to selectively bind a streptavidin molecule to them. The different photonic sensing responses obtained toward the target oligonucleotide detection, when the streptavidin molecule was bound to the MB probes or not, demonstrate the conformational change suffered by the MB upon hybridization, which promotes the displacement of the streptavidin molecule away from the surface of the photonic sensing structure. |
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