Different roles of Nrf2 and NFkB in the antioxidant imbalance produced by esculetin or quercetin on NB4 leukemia cells.

<p>Measurement of metabolic activity (MTT assay), cell cycle arrest, reactive oxygen species, expression of Nrf2 and NFkB proteins in nucleus and cytosol, expression of superoxide dismutase and lipooxygenase, activity of superoxide dismutase, phosphorilation of Jnk and p38 proteins, all in NB4...

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Detalles Bibliográficos
Autores: Rubio, Virginia, García-Pérez, Ana I., Herráez, Ángel, Diez, José Carlos
Tipo de recurso: conjunto de datos
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consorcio Madroño
Repositorio:e-cienciaDatos, Repositorio de Datos del Consorcio Madroño
OAI Identifier:doi:10.21950/MMPB5K
Acceso en línea:https://doi.org/10.21950/MMPB5K
Access Level:acceso abierto
Palabra clave:Medicine, Health and Life Sciences
Antioxidants
Apoptosis
Esculetin
Quercetin
Hydrogen peroxide
Reactive oxygen species
NB4 cell line
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network_acronym_str ES
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spelling Different roles of Nrf2 and NFkB in the antioxidant imbalance produced by esculetin or quercetin on NB4 leukemia cells.Rubio, VirginiaGarcía-Pérez, Ana I.Herráez, ÁngelDiez, José CarlosMedicine, Health and Life SciencesAntioxidantsApoptosisEsculetinQuercetinHydrogen peroxideReactive oxygen speciesNB4 cell line<p>Measurement of metabolic activity (MTT assay), cell cycle arrest, reactive oxygen species, expression of Nrf2 and NFkB proteins in nucleus and cytosol, expression of superoxide dismutase and lipooxygenase, activity of superoxide dismutase, phosphorilation of Jnk and p38 proteins, all in NB4 leukemia cell line subjected to treatments with esculetin or quercetin.</p> <p>Esculetin and quercetin are compounds that could change the balance of redox homeostasis. Treatment of NB4 leukemia cells with quercetin or esculetin affected the distribution of pro-inflammatory NFkB p65 in the nucleus and cytosol. This could suggest formation of inhibitory p50 homodimers possibly related with anti-inflammatory response. Lipoxygenase and superoxide dismutase expression were also affected by esculetin and quercetin. All these data support a relevant differential role for NFkB and Nrf2 in antiinflammatory and redox response when apoptosis was induced by esculetin or quercetin in human leukemia NB4 cells.</p>e-cienciaDatosHerráez, Ángel2024info:eu-repo/semantics/datasetinfo:eu-repo/semantics/publishedVersiontext/plainapplication/vnd.oasis.opendocument.spreadsheethttps://doi.org/10.21950/MMPB5Kreponame:e-cienciaDatos, Repositorio de Datos del Consorcio Madroñoinstname:Consorcio MadroñoEspañolInglésinfo:eu-repo/grantAgreement/Universidad de Alcalá/CCG10-UAH%2FSAL-5966/info:eu-repo/grantAgreement/Universidad de Alcalá/UAH2011%2FBIO-006/info:eu-repo/grantAgreement/Comunidad de Madrid/FISS PI060119/info:eu-repo/semantics/openAccessCC-BY-NC-SA-4.0doi:10.21950/MMPB5K2026-05-29T06:25:11Z
dc.title.none.fl_str_mv Different roles of Nrf2 and NFkB in the antioxidant imbalance produced by esculetin or quercetin on NB4 leukemia cells.
title Different roles of Nrf2 and NFkB in the antioxidant imbalance produced by esculetin or quercetin on NB4 leukemia cells.
spellingShingle Different roles of Nrf2 and NFkB in the antioxidant imbalance produced by esculetin or quercetin on NB4 leukemia cells.
Rubio, Virginia
Medicine, Health and Life Sciences
Antioxidants
Apoptosis
Esculetin
Quercetin
Hydrogen peroxide
Reactive oxygen species
NB4 cell line
title_short Different roles of Nrf2 and NFkB in the antioxidant imbalance produced by esculetin or quercetin on NB4 leukemia cells.
title_full Different roles of Nrf2 and NFkB in the antioxidant imbalance produced by esculetin or quercetin on NB4 leukemia cells.
title_fullStr Different roles of Nrf2 and NFkB in the antioxidant imbalance produced by esculetin or quercetin on NB4 leukemia cells.
title_full_unstemmed Different roles of Nrf2 and NFkB in the antioxidant imbalance produced by esculetin or quercetin on NB4 leukemia cells.
title_sort Different roles of Nrf2 and NFkB in the antioxidant imbalance produced by esculetin or quercetin on NB4 leukemia cells.
dc.creator.none.fl_str_mv Rubio, Virginia
García-Pérez, Ana I.
Herráez, Ángel
Diez, José Carlos
author Rubio, Virginia
author_facet Rubio, Virginia
García-Pérez, Ana I.
Herráez, Ángel
Diez, José Carlos
author_role author
author2 García-Pérez, Ana I.
Herráez, Ángel
Diez, José Carlos
author2_role author
author
author
dc.contributor.none.fl_str_mv Herráez, Ángel
dc.subject.none.fl_str_mv Medicine, Health and Life Sciences
Antioxidants
Apoptosis
Esculetin
Quercetin
Hydrogen peroxide
Reactive oxygen species
NB4 cell line
topic Medicine, Health and Life Sciences
Antioxidants
Apoptosis
Esculetin
Quercetin
Hydrogen peroxide
Reactive oxygen species
NB4 cell line
description <p>Measurement of metabolic activity (MTT assay), cell cycle arrest, reactive oxygen species, expression of Nrf2 and NFkB proteins in nucleus and cytosol, expression of superoxide dismutase and lipooxygenase, activity of superoxide dismutase, phosphorilation of Jnk and p38 proteins, all in NB4 leukemia cell line subjected to treatments with esculetin or quercetin.</p> <p>Esculetin and quercetin are compounds that could change the balance of redox homeostasis. Treatment of NB4 leukemia cells with quercetin or esculetin affected the distribution of pro-inflammatory NFkB p65 in the nucleus and cytosol. This could suggest formation of inhibitory p50 homodimers possibly related with anti-inflammatory response. Lipoxygenase and superoxide dismutase expression were also affected by esculetin and quercetin. All these data support a relevant differential role for NFkB and Nrf2 in antiinflammatory and redox response when apoptosis was induced by esculetin or quercetin in human leukemia NB4 cells.</p>
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
info:eu-repo/semantics/publishedVersion
format dataset
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.21950/MMPB5K
url https://doi.org/10.21950/MMPB5K
dc.language.none.fl_str_mv Español
Inglés
language_invalid_str_mv Español
Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/Universidad de Alcalá/CCG10-UAH%2FSAL-5966/
info:eu-repo/grantAgreement/Universidad de Alcalá/UAH2011%2FBIO-006/
info:eu-repo/grantAgreement/Comunidad de Madrid/FISS PI060119/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
CC-BY-NC-SA-4.0
eu_rights_str_mv openAccess
rights_invalid_str_mv CC-BY-NC-SA-4.0
dc.format.none.fl_str_mv text/plain
application/vnd.oasis.opendocument.spreadsheet
dc.publisher.none.fl_str_mv e-cienciaDatos
publisher.none.fl_str_mv e-cienciaDatos
dc.source.none.fl_str_mv reponame:e-cienciaDatos, Repositorio de Datos del Consorcio Madroño
instname:Consorcio Madroño
instname_str Consorcio Madroño
reponame_str e-cienciaDatos, Repositorio de Datos del Consorcio Madroño
collection e-cienciaDatos, Repositorio de Datos del Consorcio Madroño
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