Different roles of Nrf2 and NFkappaB in the antioxidant imbalance produced by esculetin or quercetin on NB4 leukemia cells
Esculetin (6,7-dihydrocoumarin) and the ?avonoid quercetin (3,5,7,3?,4? pentahydroxy?avone) are compounds that could change the balance of redox homeostasis. NB4 leukemia cells were treated with 25 ?M quercetin for 24 h and with esculetin at either 100 or 500 ?M for di?erent times. Quercetin increas...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Universidad de Alcalá (UAH) |
| Repositorio: | e_Buah Biblioteca Digital Universidad de Alcalá |
| Idioma: | inglés |
| OAI Identifier: | oai:ebuah.uah.es:10017/59516 |
| Acceso en línea: | http://hdl.handle.net/10017/59516 https://dx.doi.org/10.1016/j.cbi.2018.08.015 |
| Access Level: | acceso abierto |
| Palabra clave: | Antioxidants Apoptosis Esculetin Hydrogen peroxide Reactive oxygen species Tert-butylhydroperoxide Medicina Medicine |
| Sumario: | Esculetin (6,7-dihydrocoumarin) and the ?avonoid quercetin (3,5,7,3?,4? pentahydroxy?avone) are compounds that could change the balance of redox homeostasis. NB4 leukemia cells were treated with 25 ?M quercetin for 24 h and with esculetin at either 100 or 500 ?M for di?erent times. Quercetin increased the levels of proin?ammatory NFkB p65 in the nucleus correspondingly reducing them in the cytosol. The levels of NFkB p65 decreased in the nucleus at high esculetin concentration treatments for long times (19 h), concomitantly increasing the levels of anti-in?ammatory NFkB p50 in the nucleus. This could suggest formation of inhibitory p50 homodimers possibly related with anti-in?ammatory response. Lipoxygenase expression was reduced either by esculetin or quercetin. A signi?cant increase of Nrf2 in the nucleus of NB4 cells treated with 100 ?M esculetin for 19 h was observed. Quercetin increased the levels of Nrf2 in the cytosol reducing them in the nucleus. Superoxide dismutase expression increased in NB4 cells treated with esculetin in contrast with quercetin. All these data support a relevant di?erential role for NFkB and Nrf2 in anti-in?ammatory and redox response when apoptosis was induced by esculetin or quercetin in human leukemia NB4 cells. |
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