Facemask analyses for the non-invasive detection of chronic and acute P. aeruginosa lung infections using nanoparticle-based immunoassays

Pseudomonas aeruginosa (P. aeruginosa) is a pathogen that persistently colonizes the respiratory tract of patients with chronic lung diseases. The risk of acquiring a chronic P. aeruginosa infection can be minimized by rapidly detecting the pathogen in the patient's airways and promptly adminis...

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Autores: Delgado-Cano, David, Clemente, Antonio, Adrover-Jaume, Cristina, Vaquer, Andreu, López, Meritxell, Martínez, Rocío, Roig Dols, Isabel María, Iglesias, Amanda, García-Cosío, Borja, de la Rica, Roberto
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Conselleria de Salut i Consum del Govern de les Illes Balears
Repositorio:Docusalut
Idioma:inglés
OAI Identifier:oai:docusalut.com:20.500.13003/19388
Acceso en línea:https://hdl.handle.net/20.500.13003/19388
Access Level:acceso abierto
Palabra clave:Biosensing Techniques*
Cystic Fibrosis*
Humans
Immunoassay
Lung
Masks / adverse effects
Polypropylenes
Pseudomonas Infections* / diagnosis
Pseudomonas Infections* / microbiology
Pseudomonas aeruginosa
Técnicas Biosensibles
Humanos
Inmunoensayo
Pulmón
Polipropilenos
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spelling Facemask analyses for the non-invasive detection of chronic and acute P. aeruginosa lung infections using nanoparticle-based immunoassaysDelgado-Cano, DavidClemente, AntonioAdrover-Jaume, CristinaVaquer, AndreuLópez, MeritxellMartínez, RocíoRoig Dols, Isabel MaríaIglesias, AmandaGarcía-Cosío, Borjade la Rica, RobertoBiosensing Techniques*Cystic Fibrosis*HumansImmunoassayLungMasks / adverse effectsPolypropylenesPseudomonas Infections* / diagnosisPseudomonas Infections* / microbiologyPseudomonas aeruginosaTécnicas BiosensiblesHumanosInmunoensayoPulmónPolipropilenosPseudomonas aeruginosaPseudomonas aeruginosa (P. aeruginosa) is a pathogen that persistently colonizes the respiratory tract of patients with chronic lung diseases. The risk of acquiring a chronic P. aeruginosa infection can be minimized by rapidly detecting the pathogen in the patient's airways and promptly administrating adequate antibiotics. However, the rapid detection of P. aeruginosa in the lungs involves the analysis of sputum, which is a highly complex matrix that is not always available. Here, we propose an alternative diagnosis based on analyzing breath aerosols. In this approach, nanoparticle immunosensors identify bacteria adhered to the polypropylene layer of a surgical facemask that was previously worn by the patient. A polypropylene processing protocol was optimized to ensure the efficient capture and analysis of the target pathogen. The proposed analytical platform has a theoretical limit of detection of 105 CFU mL-1 in aerosolized mock samples, and a dynamic range between 105 and 108 CFU mL-1. When tested with facemasks worn by patients, the biosensors were able to detect chronic and acute P. aeruginosa lung infections, and to differentiate them from respiratory infections caused by other pathogens. The results shown here pave the way to diagnose Pseudomonas infections at the bedside, as well as to identify the progress from chronic to acute infection.Royal Society of Chemistry20232023-08-2520232023-08-25research articlehttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.13003/19388reponame:Docusalutinstname:Conselleria de Salut i Consum del Govern de les Illes BalearsInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docusalut.com:20.500.13003/193882026-06-22T12:44:07Z
dc.title.none.fl_str_mv Facemask analyses for the non-invasive detection of chronic and acute P. aeruginosa lung infections using nanoparticle-based immunoassays
title Facemask analyses for the non-invasive detection of chronic and acute P. aeruginosa lung infections using nanoparticle-based immunoassays
spellingShingle Facemask analyses for the non-invasive detection of chronic and acute P. aeruginosa lung infections using nanoparticle-based immunoassays
Delgado-Cano, David
Biosensing Techniques*
Cystic Fibrosis*
Humans
Immunoassay
Lung
Masks / adverse effects
Polypropylenes
Pseudomonas Infections* / diagnosis
Pseudomonas Infections* / microbiology
Pseudomonas aeruginosa
Técnicas Biosensibles
Humanos
Inmunoensayo
Pulmón
Polipropilenos
Pseudomonas aeruginosa
title_short Facemask analyses for the non-invasive detection of chronic and acute P. aeruginosa lung infections using nanoparticle-based immunoassays
title_full Facemask analyses for the non-invasive detection of chronic and acute P. aeruginosa lung infections using nanoparticle-based immunoassays
title_fullStr Facemask analyses for the non-invasive detection of chronic and acute P. aeruginosa lung infections using nanoparticle-based immunoassays
title_full_unstemmed Facemask analyses for the non-invasive detection of chronic and acute P. aeruginosa lung infections using nanoparticle-based immunoassays
title_sort Facemask analyses for the non-invasive detection of chronic and acute P. aeruginosa lung infections using nanoparticle-based immunoassays
dc.creator.none.fl_str_mv Delgado-Cano, David
Clemente, Antonio
Adrover-Jaume, Cristina
Vaquer, Andreu
López, Meritxell
Martínez, Rocío
Roig Dols, Isabel María
Iglesias, Amanda
García-Cosío, Borja
de la Rica, Roberto
author Delgado-Cano, David
author_facet Delgado-Cano, David
Clemente, Antonio
Adrover-Jaume, Cristina
Vaquer, Andreu
López, Meritxell
Martínez, Rocío
Roig Dols, Isabel María
Iglesias, Amanda
García-Cosío, Borja
de la Rica, Roberto
author_role author
author2 Clemente, Antonio
Adrover-Jaume, Cristina
Vaquer, Andreu
López, Meritxell
Martínez, Rocío
Roig Dols, Isabel María
Iglesias, Amanda
García-Cosío, Borja
de la Rica, Roberto
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Biosensing Techniques*
Cystic Fibrosis*
Humans
Immunoassay
Lung
Masks / adverse effects
Polypropylenes
Pseudomonas Infections* / diagnosis
Pseudomonas Infections* / microbiology
Pseudomonas aeruginosa
Técnicas Biosensibles
Humanos
Inmunoensayo
Pulmón
Polipropilenos
Pseudomonas aeruginosa
topic Biosensing Techniques*
Cystic Fibrosis*
Humans
Immunoassay
Lung
Masks / adverse effects
Polypropylenes
Pseudomonas Infections* / diagnosis
Pseudomonas Infections* / microbiology
Pseudomonas aeruginosa
Técnicas Biosensibles
Humanos
Inmunoensayo
Pulmón
Polipropilenos
Pseudomonas aeruginosa
description Pseudomonas aeruginosa (P. aeruginosa) is a pathogen that persistently colonizes the respiratory tract of patients with chronic lung diseases. The risk of acquiring a chronic P. aeruginosa infection can be minimized by rapidly detecting the pathogen in the patient's airways and promptly administrating adequate antibiotics. However, the rapid detection of P. aeruginosa in the lungs involves the analysis of sputum, which is a highly complex matrix that is not always available. Here, we propose an alternative diagnosis based on analyzing breath aerosols. In this approach, nanoparticle immunosensors identify bacteria adhered to the polypropylene layer of a surgical facemask that was previously worn by the patient. A polypropylene processing protocol was optimized to ensure the efficient capture and analysis of the target pathogen. The proposed analytical platform has a theoretical limit of detection of 105 CFU mL-1 in aerosolized mock samples, and a dynamic range between 105 and 108 CFU mL-1. When tested with facemasks worn by patients, the biosensors were able to detect chronic and acute P. aeruginosa lung infections, and to differentiate them from respiratory infections caused by other pathogens. The results shown here pave the way to diagnose Pseudomonas infections at the bedside, as well as to identify the progress from chronic to acute infection.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-08-25
2023
2023-08-25
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.13003/19388
url https://hdl.handle.net/20.500.13003/19388
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Docusalut
instname:Conselleria de Salut i Consum del Govern de les Illes Balears
instname_str Conselleria de Salut i Consum del Govern de les Illes Balears
reponame_str Docusalut
collection Docusalut
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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