Real-time protein and cell binding measurements on hydroxyapatite coatings

Although a lot of in vitro and in vivo assays have been performed during the last few decades years for hydroxyapatite bioactive coatings, there is a lack of exploitation of real-time in vitro interaction measurements. In the present work, real-time interactions for a plasma sprayed hydroxyapatite c...

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Autores: Martín Vilardell, Anna, Cinca i Luis, Núria, Jokinen, A., Garcia Giralt, Natàlia, Dosta Parras, Sergi, García Cano, Irene, Guilemany, J. M. (José María)
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/157478
Acceso en línea:https://hdl.handle.net/2445/157478
Access Level:acceso abierto
Palabra clave:Biosensors
Adsorció
Materials biomèdics
Adsorption
Biomedical materials
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spelling Real-time protein and cell binding measurements on hydroxyapatite coatingsMartín Vilardell, AnnaCinca i Luis, NúriaJokinen, A.Garcia Giralt, NatàliaDosta Parras, SergiGarcía Cano, IreneGuilemany, J. M. (José María)BiosensorsAdsorcióMaterials biomèdicsBiosensorsAdsorptionBiomedical materialsAlthough a lot of in vitro and in vivo assays have been performed during the last few decades years for hydroxyapatite bioactive coatings, there is a lack of exploitation of real-time in vitro interaction measurements. In the present work, real-time interactions for a plasma sprayed hydroxyapatite coating were measured by a Multi-Parametric Surface Plasmon Resonance (MP-SPR), and the results were compared with standard traditional cell viability in vitro assays. MP-SPR is proven to be suitable not only for measurement of molecule-molecule interactions but also molecule-material interaction measurements and cell interaction. Although SPR is extensively utilized in interaction studies, recent research of protein or cell adsorption on hydroxyapatite coatings for prostheses applications was not found. The as-sprayed hydroxyapatite coating resulted in 62.4% of crystalline phase and an average thickness of 24 ± 6 μm. The MP-SPR was used to measure lysozyme protein and human mesenchymal stem cells interaction to the hydroxyapatite coating. A comparison between the standard gold sensor and Hydroxyapatite (HA)-plasma coated sensor denoted a clearly favourable cell attachment on HA coated sensor as a significantly higher signal of cell binding was detected. Moreover, traditional cell viability and proliferation tests showed increased activity with culture time indicating that cells were proliferating on HA coating. Cells show homogeneous distribution and proliferation along the HA surface between one and seven days with no significant mortality. Cells were flattened and spread on rough surfaces from the first day, with increasing cytoplasmatic extensions during the culture time.MDPI2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/157478Articles publicats en revistes (Ciència dels Materials i Química Física)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/jfb7030023Journal of Functional Biomaterials , 2016, vol. 7, num. 3https://doi.org/10.3390/jfb7030023cc-by (c) Martín Vilardell, Anna et al., 2016http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1574782026-05-27T06:46:51Z
dc.title.none.fl_str_mv Real-time protein and cell binding measurements on hydroxyapatite coatings
title Real-time protein and cell binding measurements on hydroxyapatite coatings
spellingShingle Real-time protein and cell binding measurements on hydroxyapatite coatings
Martín Vilardell, Anna
Biosensors
Adsorció
Materials biomèdics
Biosensors
Adsorption
Biomedical materials
title_short Real-time protein and cell binding measurements on hydroxyapatite coatings
title_full Real-time protein and cell binding measurements on hydroxyapatite coatings
title_fullStr Real-time protein and cell binding measurements on hydroxyapatite coatings
title_full_unstemmed Real-time protein and cell binding measurements on hydroxyapatite coatings
title_sort Real-time protein and cell binding measurements on hydroxyapatite coatings
dc.creator.none.fl_str_mv Martín Vilardell, Anna
Cinca i Luis, Núria
Jokinen, A.
Garcia Giralt, Natàlia
Dosta Parras, Sergi
García Cano, Irene
Guilemany, J. M. (José María)
author Martín Vilardell, Anna
author_facet Martín Vilardell, Anna
Cinca i Luis, Núria
Jokinen, A.
Garcia Giralt, Natàlia
Dosta Parras, Sergi
García Cano, Irene
Guilemany, J. M. (José María)
author_role author
author2 Cinca i Luis, Núria
Jokinen, A.
Garcia Giralt, Natàlia
Dosta Parras, Sergi
García Cano, Irene
Guilemany, J. M. (José María)
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Biosensors
Adsorció
Materials biomèdics
Biosensors
Adsorption
Biomedical materials
topic Biosensors
Adsorció
Materials biomèdics
Biosensors
Adsorption
Biomedical materials
description Although a lot of in vitro and in vivo assays have been performed during the last few decades years for hydroxyapatite bioactive coatings, there is a lack of exploitation of real-time in vitro interaction measurements. In the present work, real-time interactions for a plasma sprayed hydroxyapatite coating were measured by a Multi-Parametric Surface Plasmon Resonance (MP-SPR), and the results were compared with standard traditional cell viability in vitro assays. MP-SPR is proven to be suitable not only for measurement of molecule-molecule interactions but also molecule-material interaction measurements and cell interaction. Although SPR is extensively utilized in interaction studies, recent research of protein or cell adsorption on hydroxyapatite coatings for prostheses applications was not found. The as-sprayed hydroxyapatite coating resulted in 62.4% of crystalline phase and an average thickness of 24 ± 6 μm. The MP-SPR was used to measure lysozyme protein and human mesenchymal stem cells interaction to the hydroxyapatite coating. A comparison between the standard gold sensor and Hydroxyapatite (HA)-plasma coated sensor denoted a clearly favourable cell attachment on HA coated sensor as a significantly higher signal of cell binding was detected. Moreover, traditional cell viability and proliferation tests showed increased activity with culture time indicating that cells were proliferating on HA coating. Cells show homogeneous distribution and proliferation along the HA surface between one and seven days with no significant mortality. Cells were flattened and spread on rough surfaces from the first day, with increasing cytoplasmatic extensions during the culture time.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/157478
url https://hdl.handle.net/2445/157478
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/jfb7030023
Journal of Functional Biomaterials , 2016, vol. 7, num. 3
https://doi.org/10.3390/jfb7030023
dc.rights.none.fl_str_mv cc-by (c) Martín Vilardell, Anna et al., 2016
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Martín Vilardell, Anna et al., 2016
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Ciència dels Materials i Química Física)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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