HR and chromatin assembly requirements for RFA1-MN cell viability in the absence and presence of calcium
figure posted on 2025-04-01, 19:51 authored by Ana Amiama-Roig, Marta Barrientos-Moreno, Esther Cruz-Zambrano, Luz M. López-Ruiz, Román González-Prieto, Gabriel Ríos-Orelogio, Félix Prado (A, C, and D) Effect of the indicated mutations in the growth of RFA1-MN cells as determined by spotting 10-fold...
| Autores: | , , , , , , |
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| Tipo de recurso: | conjunto de datos |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/391865 |
| Acceso en línea: | http://hdl.handle.net/10261/391865 https://digital.csic.es/handle/10261/391851 |
| Access Level: | acceso abierto |
| Palabra clave: | sister chromatid cohesion core homologous recombination active origins facilitates |
| Sumario: | figure posted on 2025-04-01, 19:51 authored by Ana Amiama-Roig, Marta Barrientos-Moreno, Esther Cruz-Zambrano, Luz M. López-Ruiz, Román González-Prieto, Gabriel Ríos-Orelogio, Félix Prado (A, C, and D) Effect of the indicated mutations in the growth of RFA1-MN cells as determined by spotting 10-fold serial dilutions of the same number of mid-log growing cells onto SMM medium without or with the indicated concentrations of CaCl2. (B) DSB sensitivity of rtt105∆ cells to HO-induced DSBs, as determined by spotting 10-fold serial dilutions of the same number of mid-log growing cells. Cells were transformed with pGAL-HO and grown in glucose (GAL1p repression) and galactose-containing medium (GAL1p activation). Wild-type and rad52∆ cells were included as control. The analyses were repeated at least twice with similar results. Mutants scored in the SGA screening are shown in bold. |
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