The effect of by-pass linseed oil supplementation on the maternal antioxidant system during the embryo-maternal recognition period in ewes

This study analyzed the effects of dietary supplementation with by-pass linseed oil (LO; rich in α-linolenic acid) on maternal antioxidant systems at Days 14 and 16 of pregnancy in Sarda ewes. This trial used sixteen dry ewes. Eight ewes (CT group) were fed with a control diet without LO, and eight...

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Autores: Contreras Solís, Ignacio, Pasciu, Valeria, Porcu, Cristian, Sotgiu, Francesca Daniela, Todorova, Neda, Baralla, Elena, Mara, Laura, Gallus, Marilia, Cabiddu, Andrea, Dattena, Maria, Abecia, José Alfonso, Berlinguer, Fiammetta
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/56543
Acceso en línea:https://hdl.handle.net/2454/56543
Access Level:acceso abierto
Palabra clave:Linseed oil
Antioxidant system
Reproductive tissues
Polyunsaturated fatty acids
Mature-dry ewes
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spelling The effect of by-pass linseed oil supplementation on the maternal antioxidant system during the embryo-maternal recognition period in ewesContreras Solís, IgnacioPasciu, ValeriaPorcu, CristianSotgiu, Francesca DanielaTodorova, NedaBaralla, ElenaMara, LauraGallus, MariliaCabiddu, AndreaDattena, MariaAbecia, José AlfonsoBerlinguer, FiammettaLinseed oilAntioxidant systemReproductive tissuesPolyunsaturated fatty acidsMature-dry ewesThis study analyzed the effects of dietary supplementation with by-pass linseed oil (LO; rich in α-linolenic acid) on maternal antioxidant systems at Days 14 and 16 of pregnancy in Sarda ewes. This trial used sixteen dry ewes. Eight ewes (CT group) were fed with a control diet without LO, and eight ewes (LO group) were fed with a diet supplemented with LO (10.8 g of α-linolenic acid/ewe/day). Both diets had similar crude protein and energy levels. The experiment included 10 days of an adaptation period and 31 days of a supplementation period. This supplementation period was divided into Period −2 (from Day −15 to −8), Period −1 (from Day −7 to −1; before synchronized mating period/Day 0), Period +1 (from Day +1 to + 7 after mating), and Period +2 (from Day +8 to +15 after mating). Estrous synchronization was induced in all the ewes using an intravaginal sponge (45 mg fluorgestone acetate) for 14 days and equine chorionic gonadotropin (350 UI/ewe) at the end of the treatment. On Days 14 (CT, N = 4; LO, N = 4) and 16 (CT, N = 4; LO, N = 4) after mating, the ewes were slaughtered. Samples of plasma, uterine, and luteal tissues were collected. Thiols, total antioxidant activity (TEAC), superoxide dismutase (SOD) activity, and malondialdehyde (MDA) content were measured. On Day 16, thiol and TEAC in luteal tissues were higher in the LO group when compared with the control one (p < 0.05). Moreover, TEAC was higher for the LO group in uterine tissues on Days 14 and 16 (p < 0.05). SOD activity was higher in the LO group in luteal and uterine tissues on Day 14 and Day 16, respectively (p < 0.001). On Day 16, uterine MDA content was lower for the LO group (p < 0.001). No differences were found between groups at the plasmatic level. However, the by-pass LO supplementation enhanced the analyzed antioxidant parameters in luteal and uterine tissues. In conclusion, these results demonstrate that by-pass LO supplementation exerted a positive effect on antioxidative defenses on maternal structures during the embryo-maternal recognition period in ewes. Thus, this could contribute to improving the maternal environment during the embryo-maternal recognition period in mammals.This study was supported by H2020-Marie-Sklodowska-Curie Actions-IF-2018-NUTREPHEALTH-832326.MDPIAgronomía, Biotecnología y AlimentaciónAgronomia, Bioteknologia eta Elikadura2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2454/56543reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglésinfo:eu-repo/grantAgreement/European Commission/Horizon 2020 Framework Programme/832326© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/565432026-06-17T12:41:47Z
dc.title.none.fl_str_mv The effect of by-pass linseed oil supplementation on the maternal antioxidant system during the embryo-maternal recognition period in ewes
title The effect of by-pass linseed oil supplementation on the maternal antioxidant system during the embryo-maternal recognition period in ewes
spellingShingle The effect of by-pass linseed oil supplementation on the maternal antioxidant system during the embryo-maternal recognition period in ewes
Contreras Solís, Ignacio
Linseed oil
Antioxidant system
Reproductive tissues
Polyunsaturated fatty acids
Mature-dry ewes
title_short The effect of by-pass linseed oil supplementation on the maternal antioxidant system during the embryo-maternal recognition period in ewes
title_full The effect of by-pass linseed oil supplementation on the maternal antioxidant system during the embryo-maternal recognition period in ewes
title_fullStr The effect of by-pass linseed oil supplementation on the maternal antioxidant system during the embryo-maternal recognition period in ewes
title_full_unstemmed The effect of by-pass linseed oil supplementation on the maternal antioxidant system during the embryo-maternal recognition period in ewes
title_sort The effect of by-pass linseed oil supplementation on the maternal antioxidant system during the embryo-maternal recognition period in ewes
dc.creator.none.fl_str_mv Contreras Solís, Ignacio
Pasciu, Valeria
Porcu, Cristian
Sotgiu, Francesca Daniela
Todorova, Neda
Baralla, Elena
Mara, Laura
Gallus, Marilia
Cabiddu, Andrea
Dattena, Maria
Abecia, José Alfonso
Berlinguer, Fiammetta
author Contreras Solís, Ignacio
author_facet Contreras Solís, Ignacio
Pasciu, Valeria
Porcu, Cristian
Sotgiu, Francesca Daniela
Todorova, Neda
Baralla, Elena
Mara, Laura
Gallus, Marilia
Cabiddu, Andrea
Dattena, Maria
Abecia, José Alfonso
Berlinguer, Fiammetta
author_role author
author2 Pasciu, Valeria
Porcu, Cristian
Sotgiu, Francesca Daniela
Todorova, Neda
Baralla, Elena
Mara, Laura
Gallus, Marilia
Cabiddu, Andrea
Dattena, Maria
Abecia, José Alfonso
Berlinguer, Fiammetta
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agronomía, Biotecnología y Alimentación
Agronomia, Bioteknologia eta Elikadura
dc.subject.none.fl_str_mv Linseed oil
Antioxidant system
Reproductive tissues
Polyunsaturated fatty acids
Mature-dry ewes
topic Linseed oil
Antioxidant system
Reproductive tissues
Polyunsaturated fatty acids
Mature-dry ewes
description This study analyzed the effects of dietary supplementation with by-pass linseed oil (LO; rich in α-linolenic acid) on maternal antioxidant systems at Days 14 and 16 of pregnancy in Sarda ewes. This trial used sixteen dry ewes. Eight ewes (CT group) were fed with a control diet without LO, and eight ewes (LO group) were fed with a diet supplemented with LO (10.8 g of α-linolenic acid/ewe/day). Both diets had similar crude protein and energy levels. The experiment included 10 days of an adaptation period and 31 days of a supplementation period. This supplementation period was divided into Period −2 (from Day −15 to −8), Period −1 (from Day −7 to −1; before synchronized mating period/Day 0), Period +1 (from Day +1 to + 7 after mating), and Period +2 (from Day +8 to +15 after mating). Estrous synchronization was induced in all the ewes using an intravaginal sponge (45 mg fluorgestone acetate) for 14 days and equine chorionic gonadotropin (350 UI/ewe) at the end of the treatment. On Days 14 (CT, N = 4; LO, N = 4) and 16 (CT, N = 4; LO, N = 4) after mating, the ewes were slaughtered. Samples of plasma, uterine, and luteal tissues were collected. Thiols, total antioxidant activity (TEAC), superoxide dismutase (SOD) activity, and malondialdehyde (MDA) content were measured. On Day 16, thiol and TEAC in luteal tissues were higher in the LO group when compared with the control one (p < 0.05). Moreover, TEAC was higher for the LO group in uterine tissues on Days 14 and 16 (p < 0.05). SOD activity was higher in the LO group in luteal and uterine tissues on Day 14 and Day 16, respectively (p < 0.001). On Day 16, uterine MDA content was lower for the LO group (p < 0.001). No differences were found between groups at the plasmatic level. However, the by-pass LO supplementation enhanced the analyzed antioxidant parameters in luteal and uterine tissues. In conclusion, these results demonstrate that by-pass LO supplementation exerted a positive effect on antioxidative defenses on maternal structures during the embryo-maternal recognition period in ewes. Thus, this could contribute to improving the maternal environment during the embryo-maternal recognition period in mammals.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/56543
url https://hdl.handle.net/2454/56543
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/European Commission/Horizon 2020 Framework Programme/832326
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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