Advanced Binary Guanosine and Guanosine 5'‑Monophosphate Cell-Laden Hydrogels for Soft Tissue Reconstruction by 3D Bioprinting [Dataset]
Soft tissue defects or pathologies frequently necessitate the use of biomaterials that provide the volume required for subsequent vascularization and tissue formation as autrografts are not always a feasible alternative. Supramolecular hydrogels represent promising candidates because of their 3D str...
| Autores: | , , , , , |
|---|---|
| Tipo de recurso: | conjunto de datos |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/356211 |
| Acceso en línea: | http://hdl.handle.net/10261/356211 |
| Access Level: | acceso abierto |
| Palabra clave: | Thereby potentially improving sup >+ Recent years since Present work aimed Native extracellular matrix Indicating successful differentiation Indicating excellent g Extensive nanofibrillar network Evenly distributed throughout Tissue reconstruction intervention Soft tissue reconstruction Providing enough stability Low shape stability Proper cell functioning Pathologies frequently necessitate Lipid droplet formation Hydrogels may enable Ensure scaffold biointegration 3d printing due Binary hydrogel made Laden hydrogel capable Sustain living cells Ensuring cell survival Advanced binary guanosine Binary cell Tissue formation Hydrogel scaffold Cell survival Laden hydrogels Increase stability Frequently inappropriate Quadruplex formation Printed scaffold Based hydrogels 3d structure 3d bioprinting Volume required Ultimately forming Thixotropic qualities Subsequent vascularization Printed structure Prime candidates Nucleoside self Material spreading Hyperbranched polyethylenimine Feasible alternative Coordinating k Adipogenic conditions 7 days 21 days 2000 kda |
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Advanced Binary Guanosine and Guanosine 5'‑Monophosphate Cell-Laden Hydrogels for Soft Tissue Reconstruction by 3D Bioprinting [Dataset] |
| title |
Advanced Binary Guanosine and Guanosine 5'‑Monophosphate Cell-Laden Hydrogels for Soft Tissue Reconstruction by 3D Bioprinting [Dataset] |
| spellingShingle |
Advanced Binary Guanosine and Guanosine 5'‑Monophosphate Cell-Laden Hydrogels for Soft Tissue Reconstruction by 3D Bioprinting [Dataset] Godoy-Gallardo, María Thereby potentially improving sup >+ Recent years since Present work aimed Native extracellular matrix Indicating successful differentiation Indicating excellent g Extensive nanofibrillar network Evenly distributed throughout Tissue reconstruction intervention Soft tissue reconstruction Providing enough stability Low shape stability Proper cell functioning Pathologies frequently necessitate Lipid droplet formation Hydrogels may enable Ensure scaffold biointegration 3d printing due Binary hydrogel made Laden hydrogel capable Sustain living cells Ensuring cell survival Advanced binary guanosine Binary cell Tissue formation Hydrogel scaffold Cell survival Laden hydrogels Increase stability Frequently inappropriate Quadruplex formation Printed scaffold Based hydrogels 3d structure 3d bioprinting Volume required Ultimately forming Thixotropic qualities Subsequent vascularization Printed structure Prime candidates Nucleoside self Material spreading Hyperbranched polyethylenimine Feasible alternative Coordinating k Adipogenic conditions 7 days 21 days 2000 kda |
| title_short |
Advanced Binary Guanosine and Guanosine 5'‑Monophosphate Cell-Laden Hydrogels for Soft Tissue Reconstruction by 3D Bioprinting [Dataset] |
| title_full |
Advanced Binary Guanosine and Guanosine 5'‑Monophosphate Cell-Laden Hydrogels for Soft Tissue Reconstruction by 3D Bioprinting [Dataset] |
| title_fullStr |
Advanced Binary Guanosine and Guanosine 5'‑Monophosphate Cell-Laden Hydrogels for Soft Tissue Reconstruction by 3D Bioprinting [Dataset] |
| title_full_unstemmed |
Advanced Binary Guanosine and Guanosine 5'‑Monophosphate Cell-Laden Hydrogels for Soft Tissue Reconstruction by 3D Bioprinting [Dataset] |
| title_sort |
Advanced Binary Guanosine and Guanosine 5'‑Monophosphate Cell-Laden Hydrogels for Soft Tissue Reconstruction by 3D Bioprinting [Dataset] |
| dc.creator.none.fl_str_mv |
Godoy-Gallardo, María Merino-Gómez, Maria Mateos-Timoneda, Miguel A. Eckhard, Ulrich Gil, Francisco Javier Pérez, Román A. |
| author |
Godoy-Gallardo, María |
| author_facet |
Godoy-Gallardo, María Merino-Gómez, Maria Mateos-Timoneda, Miguel A. Eckhard, Ulrich Gil, Francisco Javier Pérez, Román A. |
| author_role |
author |
| author2 |
Merino-Gómez, Maria Mateos-Timoneda, Miguel A. Eckhard, Ulrich Gil, Francisco Javier Pérez, Román A. |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Thereby potentially improving sup >+ Recent years since Present work aimed Native extracellular matrix Indicating successful differentiation Indicating excellent g Extensive nanofibrillar network Evenly distributed throughout Tissue reconstruction intervention Soft tissue reconstruction Providing enough stability Low shape stability Proper cell functioning Pathologies frequently necessitate Lipid droplet formation Hydrogels may enable Ensure scaffold biointegration 3d printing due Binary hydrogel made Laden hydrogel capable Sustain living cells Ensuring cell survival Advanced binary guanosine Binary cell Tissue formation Hydrogel scaffold Cell survival Laden hydrogels Increase stability Frequently inappropriate Quadruplex formation Printed scaffold Based hydrogels 3d structure 3d bioprinting Volume required Ultimately forming Thixotropic qualities Subsequent vascularization Printed structure Prime candidates Nucleoside self Material spreading Hyperbranched polyethylenimine Feasible alternative Coordinating k Adipogenic conditions 7 days 21 days 2000 kda |
| topic |
Thereby potentially improving sup >+ Recent years since Present work aimed Native extracellular matrix Indicating successful differentiation Indicating excellent g Extensive nanofibrillar network Evenly distributed throughout Tissue reconstruction intervention Soft tissue reconstruction Providing enough stability Low shape stability Proper cell functioning Pathologies frequently necessitate Lipid droplet formation Hydrogels may enable Ensure scaffold biointegration 3d printing due Binary hydrogel made Laden hydrogel capable Sustain living cells Ensuring cell survival Advanced binary guanosine Binary cell Tissue formation Hydrogel scaffold Cell survival Laden hydrogels Increase stability Frequently inappropriate Quadruplex formation Printed scaffold Based hydrogels 3d structure 3d bioprinting Volume required Ultimately forming Thixotropic qualities Subsequent vascularization Printed structure Prime candidates Nucleoside self Material spreading Hyperbranched polyethylenimine Feasible alternative Coordinating k Adipogenic conditions 7 days 21 days 2000 kda |
| description |
Soft tissue defects or pathologies frequently necessitate the use of biomaterials that provide the volume required for subsequent vascularization and tissue formation as autrografts are not always a feasible alternative. Supramolecular hydrogels represent promising candidates because of their 3D structure, which resembles the native extracellular matrix, and their capacity to entrap and sustain living cells. Guanosine-based hydrogels have emerged as prime candidates in recent years since the nucleoside self-assembles into well-ordered structures like G-quadruplexes by coordinating K+ ions and π–π stacking, ultimately forming an extensive nanofibrillar network. However, such compositions were frequently inappropriate for 3D printing due to material spreading and low shape stability over time. Thus, the present work aimed to develop a binary cell-laden hydrogel capable of ensuring cell survival while providing enough stability to ensure scaffold biointegration during soft tissue reconstruction. For that purpose, a binary hydrogel made of guanosine and guanosine 5'-monophosphate was optimized, rat mesenchymal stem cells were entrapped, and the composition was bioprinted. To further increase stability, the printed structure was coated with hyperbranched polyethylenimine. Scanning electron microscopic studies demonstrated an extensive nanofibrillar network, indicating excellent G-quadruplex formation, and rheological analysis confirmed good printing and thixotropic qualities. Additionally, diffusion tests using fluorescein isothiocyanate labeled-dextran (70, 500, and 2000 kDa) showed that nutrients of various molecular weights may diffuse through the hydrogel scaffold. Finally, cells were evenly distributed throughout the printed scaffold, cell survival was 85% after 21 days, and lipid droplet formation was observed after 7 days under adipogenic conditions, indicating successful differentiation and proper cell functioning. To conclude, such hydrogels may enable the 3D bioprinting of customized scaffolds perfectly matching the respective soft tissue defect, thereby potentially improving the outcome of the tissue reconstruction intervention. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023 2024 2024 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/dataset http://purl.org/coar/resource_type/c_ddb1 Publisher's version info:eu-repo/semantics/publishedVersion |
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dataset |
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http://hdl.handle.net/10261/356211 |
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http://hdl.handle.net/10261/356211 |
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Inglés |
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Inglés |
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Godoy-Gallardo, María; Merino-Gómez, Maria; Mateos-Timoneda, Miguel A.; Eckhard, Ulrich; Gil, Francisco Javier; Pérez, Román A. Advanced Binary Guanosine and Guanosine 5'-Monophosphate Cell-Laden Hydrogels for Soft Tissue Reconstruction by 3D Bioprinting. http://dx.doi.org/10.1021/acsami.2c23277 . http://hdl.handle.net/10261/341508 https://doi.org/10.1021/acsami.2c23277.s001 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Figshare |
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Figshare |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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Advanced Binary Guanosine and Guanosine 5'‑Monophosphate Cell-Laden Hydrogels for Soft Tissue Reconstruction by 3D Bioprinting [Dataset]Godoy-Gallardo, MaríaMerino-Gómez, MariaMateos-Timoneda, Miguel A.Eckhard, UlrichGil, Francisco JavierPérez, Román A.Thereby potentially improvingsup >+Recent years sincePresent work aimedNative extracellular matrixIndicating successful differentiationIndicating excellent gExtensive nanofibrillar networkEvenly distributed throughoutTissue reconstruction interventionSoft tissue reconstructionProviding enough stabilityLow shape stabilityProper cell functioningPathologies frequently necessitateLipid droplet formationHydrogels may enableEnsure scaffold biointegration3d printing dueBinary hydrogel madeLaden hydrogel capableSustain living cellsEnsuring cell survivalAdvanced binary guanosineBinary cellTissue formationHydrogel scaffoldCell survivalLaden hydrogelsIncrease stabilityFrequently inappropriateQuadruplex formationPrinted scaffoldBased hydrogels3d structure3d bioprintingVolume requiredUltimately formingThixotropic qualitiesSubsequent vascularizationPrinted structurePrime candidatesNucleoside selfMaterial spreadingHyperbranched polyethylenimineFeasible alternativeCoordinating kAdipogenic conditions7 days21 days2000 kdaSoft tissue defects or pathologies frequently necessitate the use of biomaterials that provide the volume required for subsequent vascularization and tissue formation as autrografts are not always a feasible alternative. Supramolecular hydrogels represent promising candidates because of their 3D structure, which resembles the native extracellular matrix, and their capacity to entrap and sustain living cells. Guanosine-based hydrogels have emerged as prime candidates in recent years since the nucleoside self-assembles into well-ordered structures like G-quadruplexes by coordinating K+ ions and π–π stacking, ultimately forming an extensive nanofibrillar network. However, such compositions were frequently inappropriate for 3D printing due to material spreading and low shape stability over time. Thus, the present work aimed to develop a binary cell-laden hydrogel capable of ensuring cell survival while providing enough stability to ensure scaffold biointegration during soft tissue reconstruction. For that purpose, a binary hydrogel made of guanosine and guanosine 5'-monophosphate was optimized, rat mesenchymal stem cells were entrapped, and the composition was bioprinted. To further increase stability, the printed structure was coated with hyperbranched polyethylenimine. Scanning electron microscopic studies demonstrated an extensive nanofibrillar network, indicating excellent G-quadruplex formation, and rheological analysis confirmed good printing and thixotropic qualities. Additionally, diffusion tests using fluorescein isothiocyanate labeled-dextran (70, 500, and 2000 kDa) showed that nutrients of various molecular weights may diffuse through the hydrogel scaffold. Finally, cells were evenly distributed throughout the printed scaffold, cell survival was 85% after 21 days, and lipid droplet formation was observed after 7 days under adipogenic conditions, indicating successful differentiation and proper cell functioning. To conclude, such hydrogels may enable the 3D bioprinting of customized scaffolds perfectly matching the respective soft tissue defect, thereby potentially improving the outcome of the tissue reconstruction intervention.Peer reviewedFigshareConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/356211reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésGodoy-Gallardo, María; Merino-Gómez, Maria; Mateos-Timoneda, Miguel A.; Eckhard, Ulrich; Gil, Francisco Javier; Pérez, Román A. Advanced Binary Guanosine and Guanosine 5'-Monophosphate Cell-Laden Hydrogels for Soft Tissue Reconstruction by 3D Bioprinting. http://dx.doi.org/10.1021/acsami.2c23277 . http://hdl.handle.net/10261/341508https://doi.org/10.1021/acsami.2c23277.s001Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3562112026-05-22T06:33:51Z |
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15.81155 |