Phloem sap proteins are part of a core stress responsive proteome involved in drought stress adjustment

Publicado 2021-01-02

Detalles Bibliográficos
Autores: Castañeda Presa, Verónica, González García, Esther, Wienkoop, Stefanie
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/52827
Acceso en línea:https://hdl.handle.net/2454/52827
Access Level:acceso abierto
Palabra clave:Phloem proteome
Drought stress
Core stress responsive proteome
Redox homeostasis
Cell signaling
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spelling Phloem sap proteins are part of a core stress responsive proteome involved in drought stress adjustmentCastañeda Presa, VerónicaGonzález García, EstherWienkoop, Stefanie Phloem proteomeDrought stressCore stress responsive proteomeRedox homeostasisCell signalingPublicado 2021-01-02During moderate drought stress, plants can adjust by changes in the protein profiles of the different organs. Plants transport and modulate extracellular stimuli local and systemically through commonly induced inter- and intracellular reactions. However, most proteins are frequently considered, cell and organelle specific. Hence, while signaling molecules and peptides can travel systemically throughout the whole plant, it is not clear, whether protein isoforms may exist ubiquitously across organs, and what function those may have during drought regulation. By applying shotgun proteomics, we extracted a core proteome of 92 identical protein isoforms, shared ubiquitously amongst several Medicago truncatula tissues, including roots, phloem sap, petioles, and leaves. We investigated their relative distribution across the different tissues and their response to moderate drought stress. In addition, we functionally compared this plant core stress responsive proteome with the organ-specific proteomes. Our study revealed plant ubiquitous protein isoforms, mainly related to redox homeostasis and signaling and involved in protein interaction networks across the whole plant. Furthermore, about 90% of these identified core protein isoforms were significantly involved in drought stress response, indicating a crucial role of the core stress responsive proteome (CSRP) in the plant organ cross-communication, important for a long-distance stress-responsive network. Besides, the data allowed for a comprehensive characterization of the phloem proteome, revealing new insights into its function. For instance, CSRP protein levels involved in stress and redox are relatively more abundant in the phloem compared to the other tissues already under control conditions. This suggests a major role of the phloem in stress protection and antioxidant activity enabling the plants metabolic maintenance and rapid response upon moderate stress. We anticipate our study to be a starting point for future investigations of the role of the core plant proteome. Under an evolutionary perspective, CSRP would enable communication of different cells with each other and the environment being crucial for coordinated stress response of multicellular organisms.VC was funded by the Basque Country Government (BFI-2012-97). This work was financed by the Public University of Navarra/Caja Navarra Foundation (7442-1941/2016) and the University of Vienna (mass spectrometry facility). We are grateful for the open access publication fees funded by the University of Vienna.Frontiers MediaCienciasZientziakInstitute for Multidisciplinary Research in Applied Biology - IMABUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoa2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/ziphttps://hdl.handle.net/2454/52827reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglés© 2021 Castañeda, González and Wienkoop. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/528272026-06-17T12:41:47Z
dc.title.none.fl_str_mv Phloem sap proteins are part of a core stress responsive proteome involved in drought stress adjustment
title Phloem sap proteins are part of a core stress responsive proteome involved in drought stress adjustment
spellingShingle Phloem sap proteins are part of a core stress responsive proteome involved in drought stress adjustment
Castañeda Presa, Verónica
Phloem proteome
Drought stress
Core stress responsive proteome
Redox homeostasis
Cell signaling
title_short Phloem sap proteins are part of a core stress responsive proteome involved in drought stress adjustment
title_full Phloem sap proteins are part of a core stress responsive proteome involved in drought stress adjustment
title_fullStr Phloem sap proteins are part of a core stress responsive proteome involved in drought stress adjustment
title_full_unstemmed Phloem sap proteins are part of a core stress responsive proteome involved in drought stress adjustment
title_sort Phloem sap proteins are part of a core stress responsive proteome involved in drought stress adjustment
dc.creator.none.fl_str_mv Castañeda Presa, Verónica
González García, Esther
Wienkoop, Stefanie
author Castañeda Presa, Verónica
author_facet Castañeda Presa, Verónica
González García, Esther
Wienkoop, Stefanie
author_role author
author2 González García, Esther
Wienkoop, Stefanie
author2_role author
author
dc.contributor.none.fl_str_mv Ciencias
Zientziak
Institute for Multidisciplinary Research in Applied Biology - IMAB
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
dc.subject.none.fl_str_mv Phloem proteome
Drought stress
Core stress responsive proteome
Redox homeostasis
Cell signaling
topic Phloem proteome
Drought stress
Core stress responsive proteome
Redox homeostasis
Cell signaling
description Publicado 2021-01-02
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/52827
url https://hdl.handle.net/2454/52827
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/zip
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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