A phloem-localized Arabidopsis metacaspase (AtMC3) improves drought tolerance

Increasing drought phenomena pose a serious threat to agricultural productivity. Although plants have multiple ways to respond to the complexity of drought stress, the underlying mechanisms of stress sensing and signaling remain unclear. The role of the vasculature, in particular the phloem, in faci...

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Detalles Bibliográficos
Autores: Pitsili, Eugenia, Rodriguez-Trevino, Ricardo, Ruiz-Solani, Nerea, Demir, Fatih, Kastanaki, Elizabeth, Dambire, Charlene, Pedro-Jové, Roger de, Vercammen, Dominique, Salguero-Linares, José Manuel, Hall, Hardy, Mantz, Melissa, Schuler, Martin, Tuominen, Hannele, Breusegem, Frank Van, Valls, Marc, Munné-Bosch, Sergi, Holdsworth, Michael J., Huesgen, Pitter F., Rodríguez-Villalón, Antia, Coll, Núria S.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/343137
Acceso en línea:http://hdl.handle.net/10261/343137
Access Level:acceso abierto
Palabra clave:Abscisic acid
Arabidopsis thaliana
Drought
Hypoxia
Metacaspases
Osmotic stress
Phloem
Descripción
Sumario:Increasing drought phenomena pose a serious threat to agricultural productivity. Although plants have multiple ways to respond to the complexity of drought stress, the underlying mechanisms of stress sensing and signaling remain unclear. The role of the vasculature, in particular the phloem, in facilitating inter-organ communication is critical and poorly understood. Combining genetic, proteomic and physiological approaches, we investigated the role of AtMC3, a phloem-specific member of the metacaspase family, in osmotic stress responses in Arabidopsis thaliana. Analyses of the proteome in plants with altered AtMC3 levels revealed differential abundance of proteins related to osmotic stress pointing into a role of the protein in water-stress-related responses. Overexpression of AtMC3 conferred drought tolerance by enhancing the differentiation of specific vascular tissues and maintaining higher levels of vascular-mediated transportation, while plants lacking the protein showed an impaired response to drought and inability to respond effectively to the hormone abscisic acid. Overall, our data highlight the importance of AtMC3 and vascular plasticity in fine-tuning early drought responses at the whole plant level without affecting growth or yield.

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