On the Move-Sensitive Fluorescent Aptassay on Board Catalytic Micromotors for the Determination of Interleukin-6 in Ultra-Low Serum Volumes for Neonatal Sepsis Diagnostics

A graphene oxide/nickel/platinum nanoparticle micromotor (MM)-based fluorescent aptassay is proposed to determine interleukin-6 (IL-6) in serum samples from low-birth-weight infants (gestational age of less than 32 weeks and birthweight below 1000 g) with sepsis suspicion. In this kind of patients,...

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Autores: Gordón, José, Arruza Gómez, Luis, Ibáñez, María Dolores, Moreno Guzmán, María, López, Miguel Ángel, Escarpa, Alberto
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/92840
Acceso en línea:https://hdl.handle.net/20.500.14352/92840
Access Level:acceso abierto
Palabra clave:615:54
615.31
Micromotors
Neonate sepsis
Interleukin-6
Fluorescence microscopy
Graphene
On-the-move aptassay
Química farmaceútica
2301 Química Analítica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/92840
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network_name_str España
repository_id_str
spelling On the Move-Sensitive Fluorescent Aptassay on Board Catalytic Micromotors for the Determination of Interleukin-6 in Ultra-Low Serum Volumes for Neonatal Sepsis DiagnosticsGordón, JoséArruza Gómez, LuisIbáñez, María DoloresMoreno Guzmán, MaríaLópez, Miguel ÁngelEscarpa, Alberto615:54615.31MicromotorsNeonate sepsisInterleukin-6Fluorescence microscopyGrapheneOn-the-move aptassayQuímica farmaceútica2301 Química AnalíticaA graphene oxide/nickel/platinum nanoparticle micromotor (MM)-based fluorescent aptassay is proposed to determine interleukin-6 (IL-6) in serum samples from low-birth-weight infants (gestational age of less than 32 weeks and birthweight below 1000 g) with sepsis suspicion. In this kind of patients, IL-6 has demonstrated good sensitivity and specificity for the diagnosis of sepsis, both for early and late onset sepsis. The approach was based on the adsorption of the aptamer for IL6 tagged with 6-FAM as a fluorescent label (AptIL‑6, λem = 520 nm) on the graphene oxide external layer (MMGO−AptIL‑6) inducing fluorescence quenching (OFF state) and a subsequent on-the-move affinity recognition of IL-6 from AptIL‑6 (IL-6−AptIL‑6 complex) recovering the fluorescence (ON state). An aptamer against IL-6 was selected and developed by the systematic evolution of ligands by exponential enrichment technology. This approach displayed a suitable linear range of 0.07−1000 pg mL−1 (r = 0.995) covering the cut-off and clinical practice levels, allowing direct determination without any dilution and simplifying the analysis as well as exhibiting an excellent sensitivity (LOD =0.02 pg mL−1) in ultralow volumes of diagnostic clinical samples (2 μL). A high agreement between IL-6 levels obtained from our MM-based approach and the method used by the Hospital was obtained (relative error < 3%). The MM-based aptassay is competitive in comparison with that of the Hospital, in terms of a significant reduction of the sample volume (15 times less) and enhanced sensitivity, employing similar analysis times. These results position MM technology with enough potential to achieve high sensitivities in low sample volumes, opening new avenues in diagnosis based on low sample volumes.Universidad Complutense de Madrid20222022-01-0120222022-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/92840reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengS2018 NMT-4349 Not availableAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 PID2020-118154GB-I00 ESMCIN AEI 10.13039REACT UE-CM2021-01 Not available Not availableopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/928402026-06-02T12:44:21Z
dc.title.none.fl_str_mv On the Move-Sensitive Fluorescent Aptassay on Board Catalytic Micromotors for the Determination of Interleukin-6 in Ultra-Low Serum Volumes for Neonatal Sepsis Diagnostics
title On the Move-Sensitive Fluorescent Aptassay on Board Catalytic Micromotors for the Determination of Interleukin-6 in Ultra-Low Serum Volumes for Neonatal Sepsis Diagnostics
spellingShingle On the Move-Sensitive Fluorescent Aptassay on Board Catalytic Micromotors for the Determination of Interleukin-6 in Ultra-Low Serum Volumes for Neonatal Sepsis Diagnostics
Gordón, José
615:54
615.31
Micromotors
Neonate sepsis
Interleukin-6
Fluorescence microscopy
Graphene
On-the-move aptassay
Química farmaceútica
2301 Química Analítica
title_short On the Move-Sensitive Fluorescent Aptassay on Board Catalytic Micromotors for the Determination of Interleukin-6 in Ultra-Low Serum Volumes for Neonatal Sepsis Diagnostics
title_full On the Move-Sensitive Fluorescent Aptassay on Board Catalytic Micromotors for the Determination of Interleukin-6 in Ultra-Low Serum Volumes for Neonatal Sepsis Diagnostics
title_fullStr On the Move-Sensitive Fluorescent Aptassay on Board Catalytic Micromotors for the Determination of Interleukin-6 in Ultra-Low Serum Volumes for Neonatal Sepsis Diagnostics
title_full_unstemmed On the Move-Sensitive Fluorescent Aptassay on Board Catalytic Micromotors for the Determination of Interleukin-6 in Ultra-Low Serum Volumes for Neonatal Sepsis Diagnostics
title_sort On the Move-Sensitive Fluorescent Aptassay on Board Catalytic Micromotors for the Determination of Interleukin-6 in Ultra-Low Serum Volumes for Neonatal Sepsis Diagnostics
dc.creator.none.fl_str_mv Gordón, José
Arruza Gómez, Luis
Ibáñez, María Dolores
Moreno Guzmán, María
López, Miguel Ángel
Escarpa, Alberto
author Gordón, José
author_facet Gordón, José
Arruza Gómez, Luis
Ibáñez, María Dolores
Moreno Guzmán, María
López, Miguel Ángel
Escarpa, Alberto
author_role author
author2 Arruza Gómez, Luis
Ibáñez, María Dolores
Moreno Guzmán, María
López, Miguel Ángel
Escarpa, Alberto
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 615:54
615.31
Micromotors
Neonate sepsis
Interleukin-6
Fluorescence microscopy
Graphene
On-the-move aptassay
Química farmaceútica
2301 Química Analítica
topic 615:54
615.31
Micromotors
Neonate sepsis
Interleukin-6
Fluorescence microscopy
Graphene
On-the-move aptassay
Química farmaceútica
2301 Química Analítica
description A graphene oxide/nickel/platinum nanoparticle micromotor (MM)-based fluorescent aptassay is proposed to determine interleukin-6 (IL-6) in serum samples from low-birth-weight infants (gestational age of less than 32 weeks and birthweight below 1000 g) with sepsis suspicion. In this kind of patients, IL-6 has demonstrated good sensitivity and specificity for the diagnosis of sepsis, both for early and late onset sepsis. The approach was based on the adsorption of the aptamer for IL6 tagged with 6-FAM as a fluorescent label (AptIL‑6, λem = 520 nm) on the graphene oxide external layer (MMGO−AptIL‑6) inducing fluorescence quenching (OFF state) and a subsequent on-the-move affinity recognition of IL-6 from AptIL‑6 (IL-6−AptIL‑6 complex) recovering the fluorescence (ON state). An aptamer against IL-6 was selected and developed by the systematic evolution of ligands by exponential enrichment technology. This approach displayed a suitable linear range of 0.07−1000 pg mL−1 (r = 0.995) covering the cut-off and clinical practice levels, allowing direct determination without any dilution and simplifying the analysis as well as exhibiting an excellent sensitivity (LOD =0.02 pg mL−1) in ultralow volumes of diagnostic clinical samples (2 μL). A high agreement between IL-6 levels obtained from our MM-based approach and the method used by the Hospital was obtained (relative error < 3%). The MM-based aptassay is competitive in comparison with that of the Hospital, in terms of a significant reduction of the sample volume (15 times less) and enhanced sensitivity, employing similar analysis times. These results position MM technology with enough potential to achieve high sensitivities in low sample volumes, opening new avenues in diagnosis based on low sample volumes.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-01
2022
2022-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/92840
url https://hdl.handle.net/20.500.14352/92840
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv S2018 NMT-4349 Not available
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 PID2020-118154GB-I00 ES
MCIN AEI 10.13039
REACT UE-CM2021-01 Not available Not available
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300724

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