Detection of cytomegalovirus in bronchoalveolar lavage fluid from immunocompromised patients with pneumonitis by viral culture and DNA quantification

To compare the detection of human cytomegalovirus (HCMV) in bronchoalveolar lavage (BAL) fluid by viral culture and quantitative polymerase chain reaction (qPCR), and to establish a viral load threshold that can identify cases of HCMV replication indicative of pneumonitis. There is currently no univ...

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Autores: Berengua, Carla|||0000-0001-9963-3809, Miró, Elisenda|||0000-0003-2781-6926, Gutiérrez, C., Sánchez, M., Mulero, A., Ramos, P., del Cuerpo Casas, Margarita|||0000-0001-7973-5496, Torrego, Alfons|||0000-0001-6624-2465, García Cadenas, Irene|||0000-0002-2994-9055, Pajares Ruiz, Virginia|||0000-0002-0916-527X, Navarro Risueño, Ferran|||0000-0002-4302-2838, Martino Bofarull, Rodrigo|||0000-0001-5143-4042, Rabella, Núria|||0000-0002-3409-0468
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:282880
Acceso en línea:https://ddd.uab.cat/record/282880
https://dx.doi.org/urn:doi:10.1016/j.jviromet.2023.114743
Access Level:acceso abierto
Palabra clave:Cytomegalovirus
HCMV
Immunosuppressed
Pneumonitis
Quantitative PCR
Viral culture
Viral load
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spelling Detection of cytomegalovirus in bronchoalveolar lavage fluid from immunocompromised patients with pneumonitis by viral culture and DNA quantificationBerengua, Carla|||0000-0001-9963-3809Miró, Elisenda|||0000-0003-2781-6926Gutiérrez, C.Sánchez, M.Mulero, A.Ramos, P.del Cuerpo Casas, Margarita|||0000-0001-7973-5496Torrego, Alfons|||0000-0001-6624-2465García Cadenas, Irene|||0000-0002-2994-9055Pajares Ruiz, Virginia|||0000-0002-0916-527XNavarro Risueño, Ferran|||0000-0002-4302-2838Martino Bofarull, Rodrigo|||0000-0001-5143-4042Rabella, Núria|||0000-0002-3409-0468CytomegalovirusHCMVImmunosuppressedPneumonitisQuantitative PCRViral cultureViral loadTo compare the detection of human cytomegalovirus (HCMV) in bronchoalveolar lavage (BAL) fluid by viral culture and quantitative polymerase chain reaction (qPCR), and to establish a viral load threshold that can identify cases of HCMV replication indicative of pneumonitis. There is currently no universal viral load cut-off to differentiate between patients with and without pneumonitis, and the interpretation of qPCR results is challenging. 176 consecutive BAL samples from immunosuppressed hosts with signs and/or symptoms of respiratory infection were prospectively studied by viral culture and qPCR. Concordant results were obtained in 81.25% of the BAL samples. The rest were discordant, as only 34% of the qPCR-positive BAL samples were positive by culture. The median HCMV load was significantly higher in culture-positive than in culture-negative BAL samples (5038 vs 178 IU/mL). Using a cut-off value of 1258 IU/mL of HCMV in BAL, pneumonia was diagnosed with a sensitivity of 76%, a specificity of 100%, a VPP of 100% and VPN of 98%, and HCMV was isolated in 100% of the BAL cultures. We found that a qPCR-negative was a quick and reliable way of ruling out HCMV pneumonitis, but a positive result did not always indicate clinically significant replication in the lung. However, an HCMV load in BAL fluid of ≥ 1258 IU/mL was always associated with disease, whereas < 200 IU/mL rarely so.Universitat Autònoma de Barcelona 22023-01-0120232023-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/282880https://dx.doi.org/urn:doi:10.1016/j.jviromet.2023.114743reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2828802026-06-06T12:50:31Z
dc.title.none.fl_str_mv Detection of cytomegalovirus in bronchoalveolar lavage fluid from immunocompromised patients with pneumonitis by viral culture and DNA quantification
title Detection of cytomegalovirus in bronchoalveolar lavage fluid from immunocompromised patients with pneumonitis by viral culture and DNA quantification
spellingShingle Detection of cytomegalovirus in bronchoalveolar lavage fluid from immunocompromised patients with pneumonitis by viral culture and DNA quantification
Berengua, Carla|||0000-0001-9963-3809
Cytomegalovirus
HCMV
Immunosuppressed
Pneumonitis
Quantitative PCR
Viral culture
Viral load
title_short Detection of cytomegalovirus in bronchoalveolar lavage fluid from immunocompromised patients with pneumonitis by viral culture and DNA quantification
title_full Detection of cytomegalovirus in bronchoalveolar lavage fluid from immunocompromised patients with pneumonitis by viral culture and DNA quantification
title_fullStr Detection of cytomegalovirus in bronchoalveolar lavage fluid from immunocompromised patients with pneumonitis by viral culture and DNA quantification
title_full_unstemmed Detection of cytomegalovirus in bronchoalveolar lavage fluid from immunocompromised patients with pneumonitis by viral culture and DNA quantification
title_sort Detection of cytomegalovirus in bronchoalveolar lavage fluid from immunocompromised patients with pneumonitis by viral culture and DNA quantification
dc.creator.none.fl_str_mv Berengua, Carla|||0000-0001-9963-3809
Miró, Elisenda|||0000-0003-2781-6926
Gutiérrez, C.
Sánchez, M.
Mulero, A.
Ramos, P.
del Cuerpo Casas, Margarita|||0000-0001-7973-5496
Torrego, Alfons|||0000-0001-6624-2465
García Cadenas, Irene|||0000-0002-2994-9055
Pajares Ruiz, Virginia|||0000-0002-0916-527X
Navarro Risueño, Ferran|||0000-0002-4302-2838
Martino Bofarull, Rodrigo|||0000-0001-5143-4042
Rabella, Núria|||0000-0002-3409-0468
author Berengua, Carla|||0000-0001-9963-3809
author_facet Berengua, Carla|||0000-0001-9963-3809
Miró, Elisenda|||0000-0003-2781-6926
Gutiérrez, C.
Sánchez, M.
Mulero, A.
Ramos, P.
del Cuerpo Casas, Margarita|||0000-0001-7973-5496
Torrego, Alfons|||0000-0001-6624-2465
García Cadenas, Irene|||0000-0002-2994-9055
Pajares Ruiz, Virginia|||0000-0002-0916-527X
Navarro Risueño, Ferran|||0000-0002-4302-2838
Martino Bofarull, Rodrigo|||0000-0001-5143-4042
Rabella, Núria|||0000-0002-3409-0468
author_role author
author2 Miró, Elisenda|||0000-0003-2781-6926
Gutiérrez, C.
Sánchez, M.
Mulero, A.
Ramos, P.
del Cuerpo Casas, Margarita|||0000-0001-7973-5496
Torrego, Alfons|||0000-0001-6624-2465
García Cadenas, Irene|||0000-0002-2994-9055
Pajares Ruiz, Virginia|||0000-0002-0916-527X
Navarro Risueño, Ferran|||0000-0002-4302-2838
Martino Bofarull, Rodrigo|||0000-0001-5143-4042
Rabella, Núria|||0000-0002-3409-0468
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Autònoma de Barcelona
dc.subject.none.fl_str_mv Cytomegalovirus
HCMV
Immunosuppressed
Pneumonitis
Quantitative PCR
Viral culture
Viral load
topic Cytomegalovirus
HCMV
Immunosuppressed
Pneumonitis
Quantitative PCR
Viral culture
Viral load
description To compare the detection of human cytomegalovirus (HCMV) in bronchoalveolar lavage (BAL) fluid by viral culture and quantitative polymerase chain reaction (qPCR), and to establish a viral load threshold that can identify cases of HCMV replication indicative of pneumonitis. There is currently no universal viral load cut-off to differentiate between patients with and without pneumonitis, and the interpretation of qPCR results is challenging. 176 consecutive BAL samples from immunosuppressed hosts with signs and/or symptoms of respiratory infection were prospectively studied by viral culture and qPCR. Concordant results were obtained in 81.25% of the BAL samples. The rest were discordant, as only 34% of the qPCR-positive BAL samples were positive by culture. The median HCMV load was significantly higher in culture-positive than in culture-negative BAL samples (5038 vs 178 IU/mL). Using a cut-off value of 1258 IU/mL of HCMV in BAL, pneumonia was diagnosed with a sensitivity of 76%, a specificity of 100%, a VPP of 100% and VPN of 98%, and HCMV was isolated in 100% of the BAL cultures. We found that a qPCR-negative was a quick and reliable way of ruling out HCMV pneumonitis, but a positive result did not always indicate clinically significant replication in the lung. However, an HCMV load in BAL fluid of ≥ 1258 IU/mL was always associated with disease, whereas < 200 IU/mL rarely so.
publishDate 2023
dc.date.none.fl_str_mv 2
2023-01-01
2023
2023-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/282880
https://dx.doi.org/urn:doi:10.1016/j.jviromet.2023.114743
url https://ddd.uab.cat/record/282880
https://dx.doi.org/urn:doi:10.1016/j.jviromet.2023.114743
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
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