Isocratic LC–DAD–FLD method for the determination of flavonoids in paprika samples by using a rapid resolution column and post-column pH change

The determination of flavonoid compounds in paprika samples has been performed by liquid chromatography in series diode array and fluorescence detection (LC–DAD–FLD), by means of a pH change to basic medium just before FLD detection. The validation of the method was performed through the establishme...

Descripción completa

Detalles Bibliográficos
Autores: Galeano Díaz , Teresa, Muñoz de la Peña, Arsenio, Monago Maraña, Olga
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universidad Nacional de Educación a Distancia
Repositorio:e-spacio. Repositorio Institucional de la UNED
Idioma:inglés
OAI Identifier:oai:e-spacio.uned.es:20.500.14468/11560
Acceso en línea:https://hdl.handle.net/20.500.14468/11560
Access Level:acceso abierto
Palabra clave:Liquid chromatography
Fluorescence detection
Paprika
Acid hydrolysis
Protected Designation of Origin
Multidimensional-partial least-squares with residual bilinearization
id ES_c69fa0336bbc64ae06b33059e2eba4f5
oai_identifier_str oai:e-spacio.uned.es:20.500.14468/11560
network_acronym_str ES
network_name_str España
repository_id_str
spelling Isocratic LC–DAD–FLD method for the determination of flavonoids in paprika samples by using a rapid resolution column and post-column pH changeGaleano Díaz , TeresaMuñoz de la Peña, ArsenioMonago Maraña, OlgaLiquid chromatographyFluorescence detectionPaprikaAcid hydrolysisProtected Designation of OriginMultidimensional-partial least-squares with residual bilinearizationThe determination of flavonoid compounds in paprika samples has been performed by liquid chromatography in series diode array and fluorescence detection (LC–DAD–FLD), by means of a pH change to basic medium just before FLD detection. The validation of the method was performed through the establishment of the external standard calibration curves and the analytical figures of merit. Limits of detection ranging from 0.006 to 0.02 mg L−1 and 0.007 to 0.09 mg L−1 were achieved using DAD and FLD detection, respectively. The experimental conditions to carry out the hydrolysis procedure to obtain flavonoid aglycones from flavonoid glycosides have been optimized applying an experimental design and the response surface methodology. The final conditions selected were 2.5 M HCl during 45 min at 85 °C. The repeatability of this procedure was assayed and relative standard deviation (RSD) values for concentration of quercetin and luteolin compounds were lower than 2%. The quantification of quercetin, luteolin and kaempferol compounds was carried out in less than 6 min in paprika samples by means of the external standard calibration. The analytes were extracted with methanol and the extracts were previously subjected to a cleanup procedure to extend the use of the chromatographic column.Elseviere-Spacio UNED20242024-05-2020162016-05-1520162016-05-15journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14468/11560reponame:e-spacio. Repositorio Institucional de la UNEDinstname:Universidad Nacional de Educación a DistanciaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0oai:e-spacio.uned.es:20.500.14468/115602026-06-06T12:38:31Z
dc.title.none.fl_str_mv Isocratic LC–DAD–FLD method for the determination of flavonoids in paprika samples by using a rapid resolution column and post-column pH change
title Isocratic LC–DAD–FLD method for the determination of flavonoids in paprika samples by using a rapid resolution column and post-column pH change
spellingShingle Isocratic LC–DAD–FLD method for the determination of flavonoids in paprika samples by using a rapid resolution column and post-column pH change
Galeano Díaz , Teresa
Liquid chromatography
Fluorescence detection
Paprika
Acid hydrolysis
Protected Designation of Origin
Multidimensional-partial least-squares with residual bilinearization
title_short Isocratic LC–DAD–FLD method for the determination of flavonoids in paprika samples by using a rapid resolution column and post-column pH change
title_full Isocratic LC–DAD–FLD method for the determination of flavonoids in paprika samples by using a rapid resolution column and post-column pH change
title_fullStr Isocratic LC–DAD–FLD method for the determination of flavonoids in paprika samples by using a rapid resolution column and post-column pH change
title_full_unstemmed Isocratic LC–DAD–FLD method for the determination of flavonoids in paprika samples by using a rapid resolution column and post-column pH change
title_sort Isocratic LC–DAD–FLD method for the determination of flavonoids in paprika samples by using a rapid resolution column and post-column pH change
dc.creator.none.fl_str_mv Galeano Díaz , Teresa
Muñoz de la Peña, Arsenio
Monago Maraña, Olga
author Galeano Díaz , Teresa
author_facet Galeano Díaz , Teresa
Muñoz de la Peña, Arsenio
Monago Maraña, Olga
author_role author
author2 Muñoz de la Peña, Arsenio
Monago Maraña, Olga
author2_role author
author
dc.contributor.none.fl_str_mv e-Spacio UNED
dc.subject.none.fl_str_mv Liquid chromatography
Fluorescence detection
Paprika
Acid hydrolysis
Protected Designation of Origin
Multidimensional-partial least-squares with residual bilinearization
topic Liquid chromatography
Fluorescence detection
Paprika
Acid hydrolysis
Protected Designation of Origin
Multidimensional-partial least-squares with residual bilinearization
description The determination of flavonoid compounds in paprika samples has been performed by liquid chromatography in series diode array and fluorescence detection (LC–DAD–FLD), by means of a pH change to basic medium just before FLD detection. The validation of the method was performed through the establishment of the external standard calibration curves and the analytical figures of merit. Limits of detection ranging from 0.006 to 0.02 mg L−1 and 0.007 to 0.09 mg L−1 were achieved using DAD and FLD detection, respectively. The experimental conditions to carry out the hydrolysis procedure to obtain flavonoid aglycones from flavonoid glycosides have been optimized applying an experimental design and the response surface methodology. The final conditions selected were 2.5 M HCl during 45 min at 85 °C. The repeatability of this procedure was assayed and relative standard deviation (RSD) values for concentration of quercetin and luteolin compounds were lower than 2%. The quantification of quercetin, luteolin and kaempferol compounds was carried out in less than 6 min in paprika samples by means of the external standard calibration. The analytes were extracted with methanol and the extracts were previously subjected to a cleanup procedure to extend the use of the chromatographic column.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-05-15
2016
2016-05-15
2024
2024-05-20
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14468/11560
url https://hdl.handle.net/20.500.14468/11560
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
http://creativecommons.org/licenses/by-nc-nd/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:e-spacio. Repositorio Institucional de la UNED
instname:Universidad Nacional de Educación a Distancia
instname_str Universidad Nacional de Educación a Distancia
reponame_str e-spacio. Repositorio Institucional de la UNED
collection e-spacio. Repositorio Institucional de la UNED
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869419088800907264
score 15.81155

Similares en LA Referencia