Combination of Liquid Chromatography with Multivariate CurveResolution-Alternating Least-Squares (MCR-ALS) in the Quantitationof Polycyclic Aromatic Hydrocarbons Present in Paprika Samples
This work presents a strategy for quantitating polycyclic aromatic hydrocarbons (PAHs) in smoked paprikasamples. For this, a liquid chromatographic method withfluorimetric detection (HPLC-FLD) was optimized. To resolve some interference co-eluting with the target analytes, the second-order multivari...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Universidad Nacional de Educación a Distancia |
| Repositorio: | e-spacio. Repositorio Institucional de la UNED |
| Idioma: | inglés |
| OAI Identifier: | oai:e-spacio.uned.es:20.500.14468/11563 |
| Acceso en línea: | https://hdl.handle.net/20.500.14468/11563 |
| Access Level: | acceso abierto |
| Palabra clave: | smoked paprika PAHs MCR-ALS HPLC fluorescence detection Multidimensional-partial least-squares with residual bilinearization |
| Sumario: | This work presents a strategy for quantitating polycyclic aromatic hydrocarbons (PAHs) in smoked paprikasamples. For this, a liquid chromatographic method withfluorimetric detection (HPLC-FLD) was optimized. To resolve some interference co-eluting with the target analytes, the second-order multivariate curve resolution-alternating least-squares (MCR-ALS) algorithm has been employed combined with this liquid chromatographic method. Among the eight PAHs quantified(fluorene, phenanthrene, anthracene, pyrene, chrysene, benzo[a]anthracene, benzo[b]fluoranthene, and benzo[a]pyrene) byHPLC-FLD, only in the case offluorene, pyrene, and benzo[b]fluoranthene was it necessary to apply the second-order algorithmfor their resolution. Limits of detection and quantitation were between 0.015 and 0.45 mg/kg and between 0.15 and 1.5 mg/kg,respectively. Good recovery results (>80%) for paprika were obtained via the complete extraction procedure, consisting of anextraction from the matrix and the cleanup of the extract by means of silica cartridges. Higher concentrations of chrysene,benzo[a]anthracene, benzo[b]fluoranthene, and benzo[a]pyrene were found in the paprika samples, with respect to the maximalamounts allowed for other spices that are under European Regulation (EU) N°2015/1933 |
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