Evidence for Involvement of the Salmonella enterica Z-Ring Assembly Factors ZapA and ZapB in Resistance to Bile

14 páginas.- 6 figuras.- 2 tablas.- referencias.- The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2021.647305/full#supplementary-material

Detalhes bibliográficos
Autores: Fernández-Fernández, Rocío, Hernández, Sara B., Puerta Fernández, Elena, Sánchez-Romero, María A., Urdaneta, Veronica, Casadesús, Josep
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/235697
Acesso em linha:http://hdl.handle.net/10261/235697
Access Level:acceso abierto
Palavra-chave:Salmonella
Bile resistance
Z-ring
Lon protease
MicA sRNA
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spelling Evidence for Involvement of the Salmonella enterica Z-Ring Assembly Factors ZapA and ZapB in Resistance to BileFernández-Fernández, RocíoHernández, Sara B.Puerta Fernández, ElenaSánchez-Romero, María A.Urdaneta, VeronicaCasadesús, JosepSalmonellaBile resistanceZ-ringLon proteaseMicA sRNA14 páginas.- 6 figuras.- 2 tablas.- referencias.- The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2021.647305/full#supplementary-materialGenes annotated as ygfE and yiiU in the genome of Salmonella enterica serovar Typhimurium encode proteins homologous to Escherichia coli cell division factors ZapA and ZapB, respectively. ZapA− and ZapB− mutants of S. enterica are bile-sensitive. The amount of zapB mRNA increases in the presence of a sublethal concentration of sodium deoxycholate (DOC) while zapA mRNA remains unaffected. Increased zapB mRNA level in the presence of DOC is not caused by upregulation of zapB transcription but by increased stability of zapB mRNA. This increase is suppressed by an hfq mutation, suggesting the involvement of a small regulatory RNA. We provide evidence that such sRNA is MicA. The ZapB protein is degraded in the presence of DOC, and degradation appears to involve the Lon protease. We propose that increased stability of zapB mRNA in the presence of DOC may counter degradation of bile-damaged ZapB, thereby providing sufficient level of functional ZapB protein to permit Z-ring assembly in the presence of bileThis study was supported by grant BIO2016-75235-P from the Spanish Ministerio de Economía, Industria y Competitividad - Agencia Estatal de Investigación and the European Regional Fund. VU was supported by a postdoctoral contract associated to grant CVI-5879 from the Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía, Spain. MS-R was supported by the VI Plan Propio de Investigación y Transferencia de la Universidad de Sevilla.Peer reviewedFrontiers MediaMinisterio de Economía, Industria y Competitividad (España)European CommissionAgencia Estatal de Investigación (España)Junta de AndalucíaUniversidad de SevillaPuerta Fernández, Elena [0000-0003-3559-6791]Sánchez-Romero, María A. [0000-0003-3098-1033]Casadesús, Josep [0000-0002-2308-293X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/235697reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-75235-Phttp://dx.doi.org/10.3389/fmicb.2021.647305Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2356972026-05-22T06:33:51Z
dc.title.none.fl_str_mv Evidence for Involvement of the Salmonella enterica Z-Ring Assembly Factors ZapA and ZapB in Resistance to Bile
title Evidence for Involvement of the Salmonella enterica Z-Ring Assembly Factors ZapA and ZapB in Resistance to Bile
spellingShingle Evidence for Involvement of the Salmonella enterica Z-Ring Assembly Factors ZapA and ZapB in Resistance to Bile
Fernández-Fernández, Rocío
Salmonella
Bile resistance
Z-ring
Lon protease
MicA sRNA
title_short Evidence for Involvement of the Salmonella enterica Z-Ring Assembly Factors ZapA and ZapB in Resistance to Bile
title_full Evidence for Involvement of the Salmonella enterica Z-Ring Assembly Factors ZapA and ZapB in Resistance to Bile
title_fullStr Evidence for Involvement of the Salmonella enterica Z-Ring Assembly Factors ZapA and ZapB in Resistance to Bile
title_full_unstemmed Evidence for Involvement of the Salmonella enterica Z-Ring Assembly Factors ZapA and ZapB in Resistance to Bile
title_sort Evidence for Involvement of the Salmonella enterica Z-Ring Assembly Factors ZapA and ZapB in Resistance to Bile
dc.creator.none.fl_str_mv Fernández-Fernández, Rocío
Hernández, Sara B.
Puerta Fernández, Elena
Sánchez-Romero, María A.
Urdaneta, Veronica
Casadesús, Josep
author Fernández-Fernández, Rocío
author_facet Fernández-Fernández, Rocío
Hernández, Sara B.
Puerta Fernández, Elena
Sánchez-Romero, María A.
Urdaneta, Veronica
Casadesús, Josep
author_role author
author2 Hernández, Sara B.
Puerta Fernández, Elena
Sánchez-Romero, María A.
Urdaneta, Veronica
Casadesús, Josep
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía, Industria y Competitividad (España)
European Commission
Agencia Estatal de Investigación (España)
Junta de Andalucía
Universidad de Sevilla
Puerta Fernández, Elena [0000-0003-3559-6791]
Sánchez-Romero, María A. [0000-0003-3098-1033]
Casadesús, Josep [0000-0002-2308-293X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Salmonella
Bile resistance
Z-ring
Lon protease
MicA sRNA
topic Salmonella
Bile resistance
Z-ring
Lon protease
MicA sRNA
description 14 páginas.- 6 figuras.- 2 tablas.- referencias.- The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2021.647305/full#supplementary-material
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/235697
url http://hdl.handle.net/10261/235697
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-75235-P
http://dx.doi.org/10.3389/fmicb.2021.647305
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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