Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification

Rodriguez Temporal, David; Alcaide Fernández de Vega, Fernando; Mareković, Ivana; O’Connor, James Anthony; Gorton, Rebecca; Ingen, Jakko van; Bossche, An van den; Héry-Arnaud, Genevieve; Beauruelle, Clémence; Orth-Höller, Dorothea; Palacios Gutiérrez, Juan José; Tudó i Vilanova, Griselda; Bou, Germán; Ceyssens, Pieter-Jan; Garrigó, Montserrat; González Martín, Julián; Greub, Gilbert; Hrabak, Jaroslav; Ingebretsen, André; Mediavilla Gradolph, Maria Concepción; Oviaño, Marina; Palop, Begoña; Pranada, Arthur B.; Quiroga, Lidia; Ruiz Serrano, Maria Jesús; Rodríguez Sánchez, Belén

Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification

The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of...

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Autores:	Rodriguez Temporal, David, Alcaide Fernández de Vega, Fernando, Mareković, Ivana, O’Connor, James Anthony, Gorton, Rebecca, Ingen, Jakko van, Bossche, An van den, Héry-Arnaud, Genevieve, Beauruelle, Clémence, Orth-Höller, Dorothea, Palacios Gutiérrez, Juan José, Tudó i Vilanova, Griselda, Bou, Germán, Ceyssens, Pieter-Jan, Garrigó, Montserrat, González Martín, Julián, Greub, Gilbert, Hrabak, Jaroslav, Ingebretsen, André, Mediavilla Gradolph, Maria Concepción, Oviaño, Marina, Palop, Begoña, Pranada, Arthur B., Quiroga, Lidia, Ruiz Serrano, Maria Jesús, Rodríguez Sánchez, Belén
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2022
País:	España
Institución:	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:	Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:	oai:recercat.cat:2445/183115
Acceso en línea:	https://hdl.handle.net/2445/183115
Access Level:	acceso abierto
Palabra clave:	Malalties del pulmó Bacteris patògens Pulmonary diseases Pathogenic bacteria

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spelling	Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identificationRodriguez Temporal, DavidAlcaide Fernández de Vega, FernandoMareković, IvanaO’Connor, James AnthonyGorton, RebeccaIngen, Jakko vanBossche, An van denHéry-Arnaud, GenevieveBeauruelle, ClémenceOrth-Höller, DorotheaPalacios Gutiérrez, Juan JoséTudó i Vilanova, GriseldaBou, GermánCeyssens, Pieter-JanGarrigó, MontserratGonzález Martín, JuliánGreub, GilbertHrabak, JaroslavIngebretsen, AndréMediavilla Gradolph, Maria ConcepciónOviaño, MarinaPalop, BegoñaPranada, Arthur B.Quiroga, LidiaRuiz Serrano, Maria JesúsRodríguez Sánchez, BelénMalalties del pulmóBacteris patògensPulmonary diseasesPathogenic bacteriaThe ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Lowenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score >= 1.6 was obtained for 100% of isolates in 5 laboratories (68.2-98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.Springer Science and Business Media LLC2022202220222022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion7 p.application/pdfhttps://hdl.handle.net/2445/183115Articles publicats en revistes (Fonaments Clínics)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1038/s41598-022-05315-7Scientific Reports, 2022, vol 12, num 1https://doi.org/10.1038/s41598-022-05315-7cc by (c) Rodriguez Temporal, David et al, 2022http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1831152026-05-29T05:05:01Z
dc.title.none.fl_str_mv	Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification
title	Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification
spellingShingle	Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification Rodriguez Temporal, David Malalties del pulmó Bacteris patògens Pulmonary diseases Pathogenic bacteria
title_short	Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification
title_full	Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification
title_fullStr	Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification
title_full_unstemmed	Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification
title_sort	Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification
dc.creator.none.fl_str_mv	Rodriguez Temporal, David Alcaide Fernández de Vega, Fernando Mareković, Ivana O’Connor, James Anthony Gorton, Rebecca Ingen, Jakko van Bossche, An van den Héry-Arnaud, Genevieve Beauruelle, Clémence Orth-Höller, Dorothea Palacios Gutiérrez, Juan José Tudó i Vilanova, Griselda Bou, Germán Ceyssens, Pieter-Jan Garrigó, Montserrat González Martín, Julián Greub, Gilbert Hrabak, Jaroslav Ingebretsen, André Mediavilla Gradolph, Maria Concepción Oviaño, Marina Palop, Begoña Pranada, Arthur B. Quiroga, Lidia Ruiz Serrano, Maria Jesús Rodríguez Sánchez, Belén
author	Rodriguez Temporal, David
author_facet	Rodriguez Temporal, David Alcaide Fernández de Vega, Fernando Mareković, Ivana O’Connor, James Anthony Gorton, Rebecca Ingen, Jakko van Bossche, An van den Héry-Arnaud, Genevieve Beauruelle, Clémence Orth-Höller, Dorothea Palacios Gutiérrez, Juan José Tudó i Vilanova, Griselda Bou, Germán Ceyssens, Pieter-Jan Garrigó, Montserrat González Martín, Julián Greub, Gilbert Hrabak, Jaroslav Ingebretsen, André Mediavilla Gradolph, Maria Concepción Oviaño, Marina Palop, Begoña Pranada, Arthur B. Quiroga, Lidia Ruiz Serrano, Maria Jesús Rodríguez Sánchez, Belén
author_role	author
author2	Alcaide Fernández de Vega, Fernando Mareković, Ivana O’Connor, James Anthony Gorton, Rebecca Ingen, Jakko van Bossche, An van den Héry-Arnaud, Genevieve Beauruelle, Clémence Orth-Höller, Dorothea Palacios Gutiérrez, Juan José Tudó i Vilanova, Griselda Bou, Germán Ceyssens, Pieter-Jan Garrigó, Montserrat González Martín, Julián Greub, Gilbert Hrabak, Jaroslav Ingebretsen, André Mediavilla Gradolph, Maria Concepción Oviaño, Marina Palop, Begoña Pranada, Arthur B. Quiroga, Lidia Ruiz Serrano, Maria Jesús Rodríguez Sánchez, Belén
author2_role	author author author author author author author author author author author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	Malalties del pulmó Bacteris patògens Pulmonary diseases Pathogenic bacteria
topic	Malalties del pulmó Bacteris patògens Pulmonary diseases Pathogenic bacteria
description	The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Lowenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score >= 1.6 was obtained for 100% of isolates in 5 laboratories (68.2-98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.
publishDate	2022
dc.date.none.fl_str_mv	2022 2022 2022 2022
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	https://hdl.handle.net/2445/183115
url	https://hdl.handle.net/2445/183115
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	Reproducció del document publicat a: https://doi.org/10.1038/s41598-022-05315-7 Scientific Reports, 2022, vol 12, num 1 https://doi.org/10.1038/s41598-022-05315-7
dc.rights.none.fl_str_mv	cc by (c) Rodriguez Temporal, David et al, 2022 http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess
rights_invalid_str_mv	cc by (c) Rodriguez Temporal, David et al, 2022 http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	7 p. application/pdf
dc.publisher.none.fl_str_mv	Springer Science and Business Media LLC
publisher.none.fl_str_mv	Springer Science and Business Media LLC
dc.source.none.fl_str_mv	Articles publicats en revistes (Fonaments Clínics) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str	Recercat. Dipósit de la Recerca de Catalunya
collection	Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15,198674

Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification

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