Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification

The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of...

Descripción completa

Detalles Bibliográficos
Autores: Rodriguez Temporal, David, Alcaide Fernández de Vega, Fernando, Mareković, Ivana, O’Connor, James Anthony, Gorton, Rebecca, Ingen, Jakko van, Bossche, An van den, Héry-Arnaud, Genevieve, Beauruelle, Clémence, Orth-Höller, Dorothea, Palacios Gutiérrez, Juan José, Tudó i Vilanova, Griselda, Bou, Germán, Ceyssens, Pieter-Jan, Garrigó, Montserrat, González Martín, Julián, Greub, Gilbert, Hrabak, Jaroslav, Ingebretsen, André, Mediavilla Gradolph, Maria Concepción, Oviaño, Marina, Palop, Begoña, Pranada, Arthur B., Quiroga, Lidia, Ruiz Serrano, Maria Jesús, Rodríguez Sánchez, Belén
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/183115
Acceso en línea:https://hdl.handle.net/2445/183115
Access Level:acceso abierto
Palabra clave:Malalties del pulmó
Bacteris patògens
Pulmonary diseases
Pathogenic bacteria
Descripción
Sumario:The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Lowenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score >= 1.6 was obtained for 100% of isolates in 5 laboratories (68.2-98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.

Similares en LA Referencia