Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria

The single and comparative intradermal tuberculin tests (SITT and CITT) are official in vivo tests for bovine tuberculosis (TB) diagnosis using bovine and avian purified protein derivatives (PPD-B and PPD-A). Infection with bacteria other than Mycobacterium tuberculosis complex (MTC) can result in n...

Descripción completa

Detalles Bibliográficos
Autores: Fernández-Veiga, Leire, Fuertes, Miguel, Geijo, María V., Pérez de Val, Bernat|||0000-0003-3127-9182, Vidal Barba, Enric|||0000-0002-4965-3286, Michelet, Lorraine|||0000-0003-2991-1660, Boschiroli, Maria Laura|||0000-0002-6705-1684, Gómez-Buendía, Alberto, Bezos, Javier, Jones, Gareth J., Vordermeier, Martin, Juste, Ramón A.|||0000-0001-6037-5873, Garrido, Joseba M., Sevilla, Iker A.|||0000-0003-3968-3390
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:272466
Acceso en línea:https://ddd.uab.cat/record/272466
https://dx.doi.org/urn:doi:10.1038/s41598-023-30147-4
Access Level:acceso abierto
Palabra clave:Immunology
Microbiology
Infectious-disease diagnostics
id ES_c4cc58a8aeb4bcabbd79d8d0500e6b2d
oai_identifier_str oai:ddd.uab.cat:272466
network_acronym_str ES
network_name_str España
repository_id_str
spelling Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteriaFernández-Veiga, LeireFuertes, MiguelGeijo, María V.Pérez de Val, Bernat|||0000-0003-3127-9182Vidal Barba, Enric|||0000-0002-4965-3286Michelet, Lorraine|||0000-0003-2991-1660Boschiroli, Maria Laura|||0000-0002-6705-1684Gómez-Buendía, AlbertoBezos, JavierJones, Gareth J.Vordermeier, MartinJuste, Ramón A.|||0000-0001-6037-5873Garrido, Joseba M.Sevilla, Iker A.|||0000-0003-3968-3390ImmunologyMicrobiologyInfectious-disease diagnosticsThe single and comparative intradermal tuberculin tests (SITT and CITT) are official in vivo tests for bovine tuberculosis (TB) diagnosis using bovine and avian purified protein derivatives (PPD-B and PPD-A). Infection with bacteria other than Mycobacterium tuberculosis complex (MTC) can result in nonspecific reactions to these tests. We evaluated the performance of the skin test with PPDs and new defined antigens in the guinea pig model. A standard dose (SD) of Rhodococcus equi, Nocardia sp., M. nonchromogenicum, M. monacense, M. intracellulare, M. avium subsp. paratuberculosis, M. avium subsp. avium, M. avium subsp. hominissuis, M. scrofulaceum, M. persicum, M. microti, M. caprae and M. bovis, and a higher dose (HD) of M. nonchromogenicum, M. monacense, M. intracellulare, M. avium subsp. paratuberculosis were tested using PPD-B, PPD-A, P22, ESAT-6-CFP-10-Rv3615c peptide cocktail long (PCL) and fusion protein (FP). The SD of R. equi, Nocardia sp., M. nonchromogenicum, M. monacense, M. intracellulare and M. avium subsp. paratuberculosis did not cause any reactions. The HD of M. nonchromogenicum, M. monacense, M. intracellulare, and M. avium subsp. paratuberculosis and the SD of M. avium subsp. hominissuis, M. scrofulaceum and M. persicum, caused nonspecific reactions (SIT). A CITT interpretation would have considered M. avium complex and M. scrofulaceum groups negative, but not all individuals from M. nonchromogenicum HD, M. monacense HD and M. persicum SD groups. Only animals exposed to M. bovis and M. caprae reacted to PCL and FP. These results support the advantage of complementing or replacing PPD-B to improve specificity without losing sensitivity. 22023-01-0120232023-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/272466https://dx.doi.org/urn:doi:10.1038/s41598-023-30147-4reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-105155RB-C33open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2724662026-06-06T12:50:31Z
dc.title.none.fl_str_mv Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria
title Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria
spellingShingle Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria
Fernández-Veiga, Leire
Immunology
Microbiology
Infectious-disease diagnostics
title_short Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria
title_full Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria
title_fullStr Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria
title_full_unstemmed Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria
title_sort Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria
dc.creator.none.fl_str_mv Fernández-Veiga, Leire
Fuertes, Miguel
Geijo, María V.
Pérez de Val, Bernat|||0000-0003-3127-9182
Vidal Barba, Enric|||0000-0002-4965-3286
Michelet, Lorraine|||0000-0003-2991-1660
Boschiroli, Maria Laura|||0000-0002-6705-1684
Gómez-Buendía, Alberto
Bezos, Javier
Jones, Gareth J.
Vordermeier, Martin
Juste, Ramón A.|||0000-0001-6037-5873
Garrido, Joseba M.
Sevilla, Iker A.|||0000-0003-3968-3390
author Fernández-Veiga, Leire
author_facet Fernández-Veiga, Leire
Fuertes, Miguel
Geijo, María V.
Pérez de Val, Bernat|||0000-0003-3127-9182
Vidal Barba, Enric|||0000-0002-4965-3286
Michelet, Lorraine|||0000-0003-2991-1660
Boschiroli, Maria Laura|||0000-0002-6705-1684
Gómez-Buendía, Alberto
Bezos, Javier
Jones, Gareth J.
Vordermeier, Martin
Juste, Ramón A.|||0000-0001-6037-5873
Garrido, Joseba M.
Sevilla, Iker A.|||0000-0003-3968-3390
author_role author
author2 Fuertes, Miguel
Geijo, María V.
Pérez de Val, Bernat|||0000-0003-3127-9182
Vidal Barba, Enric|||0000-0002-4965-3286
Michelet, Lorraine|||0000-0003-2991-1660
Boschiroli, Maria Laura|||0000-0002-6705-1684
Gómez-Buendía, Alberto
Bezos, Javier
Jones, Gareth J.
Vordermeier, Martin
Juste, Ramón A.|||0000-0001-6037-5873
Garrido, Joseba M.
Sevilla, Iker A.|||0000-0003-3968-3390
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Immunology
Microbiology
Infectious-disease diagnostics
topic Immunology
Microbiology
Infectious-disease diagnostics
description The single and comparative intradermal tuberculin tests (SITT and CITT) are official in vivo tests for bovine tuberculosis (TB) diagnosis using bovine and avian purified protein derivatives (PPD-B and PPD-A). Infection with bacteria other than Mycobacterium tuberculosis complex (MTC) can result in nonspecific reactions to these tests. We evaluated the performance of the skin test with PPDs and new defined antigens in the guinea pig model. A standard dose (SD) of Rhodococcus equi, Nocardia sp., M. nonchromogenicum, M. monacense, M. intracellulare, M. avium subsp. paratuberculosis, M. avium subsp. avium, M. avium subsp. hominissuis, M. scrofulaceum, M. persicum, M. microti, M. caprae and M. bovis, and a higher dose (HD) of M. nonchromogenicum, M. monacense, M. intracellulare, M. avium subsp. paratuberculosis were tested using PPD-B, PPD-A, P22, ESAT-6-CFP-10-Rv3615c peptide cocktail long (PCL) and fusion protein (FP). The SD of R. equi, Nocardia sp., M. nonchromogenicum, M. monacense, M. intracellulare and M. avium subsp. paratuberculosis did not cause any reactions. The HD of M. nonchromogenicum, M. monacense, M. intracellulare, and M. avium subsp. paratuberculosis and the SD of M. avium subsp. hominissuis, M. scrofulaceum and M. persicum, caused nonspecific reactions (SIT). A CITT interpretation would have considered M. avium complex and M. scrofulaceum groups negative, but not all individuals from M. nonchromogenicum HD, M. monacense HD and M. persicum SD groups. Only animals exposed to M. bovis and M. caprae reacted to PCL and FP. These results support the advantage of complementing or replacing PPD-B to improve specificity without losing sensitivity.
publishDate 2023
dc.date.none.fl_str_mv 2
2023-01-01
2023
2023-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/272466
https://dx.doi.org/urn:doi:10.1038/s41598-023-30147-4
url https://ddd.uab.cat/record/272466
https://dx.doi.org/urn:doi:10.1038/s41598-023-30147-4
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-105155RB-C33
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418933679816704
score 15.300719

Similares en LA Referencia