Isoprenoid biosynthesis in Escherichia coli via the methylerythritol phosphate pathway: enzymatic conversion of methylerythritol cyclodiphosphate into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol

A crude cell-free system from an Escherichia coli strain overexpressing the cluster containing the three genes yfgA, yfgB, and gcpE converted 2-C-methyl-d-erythritol 2,4-cyclodiphosphate (1) into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol (6), which most probably represents a nove...

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Autores: Seemann, Myriam, Campos Martínez, Narciso, Rodríguez Concepción, Manuel, Ibañez, Esther, Duvold, Tore, Tritsch, Denis, Boronat i Margosa, Albert, Rohmer, Michel
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2002
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/225926
Acceso en línea:https://hdl.handle.net/2445/225926
Access Level:acceso abierto
Palabra clave:Escheríchia coli
Enzims microbians
Escherichia coli
Microbial enzymes
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spelling Isoprenoid biosynthesis in Escherichia coli via the methylerythritol phosphate pathway: enzymatic conversion of methylerythritol cyclodiphosphate into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diolSeemann, MyriamCampos Martínez, NarcisoRodríguez Concepción, ManuelIbañez, EstherDuvold, ToreTritsch, DenisBoronat i Margosa, AlbertRohmer, MichelEscheríchia coliEnzims microbiansEscherichia coliMicrobial enzymesA crude cell-free system from an Escherichia coli strain overexpressing the cluster containing the three genes yfgA, yfgB, and gcpE converted 2-C-methyl-d-erythritol 2,4-cyclodiphosphate (1) into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol (6), which most probably represents a novel intermediate in the methylerythritol phosphate pathway for isoprenoid biosynthesis. The free diol 6 was accumulated by phosphatase treatment of the crude enzyme preparation and was identified by comparison with a synthetic reference.Elsevier Ltd.2026202620022026info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion3 p.application/pdfhttps://hdl.handle.net/2445/225926Articles publicats en revistes (Bioquímica i Biomedicina Molecular)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1016/S0040-4039(02)00003-5Tetrahedron Letters, 2002, vol. 43, num.8, p. 1413-1415https://doi.org/10.1016/S0040-4039(02)00003-5(c) Elsevier Ltd., 2002info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2259262026-05-29T05:05:01Z
dc.title.none.fl_str_mv Isoprenoid biosynthesis in Escherichia coli via the methylerythritol phosphate pathway: enzymatic conversion of methylerythritol cyclodiphosphate into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol
title Isoprenoid biosynthesis in Escherichia coli via the methylerythritol phosphate pathway: enzymatic conversion of methylerythritol cyclodiphosphate into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol
spellingShingle Isoprenoid biosynthesis in Escherichia coli via the methylerythritol phosphate pathway: enzymatic conversion of methylerythritol cyclodiphosphate into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol
Seemann, Myriam
Escheríchia coli
Enzims microbians
Escherichia coli
Microbial enzymes
title_short Isoprenoid biosynthesis in Escherichia coli via the methylerythritol phosphate pathway: enzymatic conversion of methylerythritol cyclodiphosphate into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol
title_full Isoprenoid biosynthesis in Escherichia coli via the methylerythritol phosphate pathway: enzymatic conversion of methylerythritol cyclodiphosphate into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol
title_fullStr Isoprenoid biosynthesis in Escherichia coli via the methylerythritol phosphate pathway: enzymatic conversion of methylerythritol cyclodiphosphate into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol
title_full_unstemmed Isoprenoid biosynthesis in Escherichia coli via the methylerythritol phosphate pathway: enzymatic conversion of methylerythritol cyclodiphosphate into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol
title_sort Isoprenoid biosynthesis in Escherichia coli via the methylerythritol phosphate pathway: enzymatic conversion of methylerythritol cyclodiphosphate into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol
dc.creator.none.fl_str_mv Seemann, Myriam
Campos Martínez, Narciso
Rodríguez Concepción, Manuel
Ibañez, Esther
Duvold, Tore
Tritsch, Denis
Boronat i Margosa, Albert
Rohmer, Michel
author Seemann, Myriam
author_facet Seemann, Myriam
Campos Martínez, Narciso
Rodríguez Concepción, Manuel
Ibañez, Esther
Duvold, Tore
Tritsch, Denis
Boronat i Margosa, Albert
Rohmer, Michel
author_role author
author2 Campos Martínez, Narciso
Rodríguez Concepción, Manuel
Ibañez, Esther
Duvold, Tore
Tritsch, Denis
Boronat i Margosa, Albert
Rohmer, Michel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Escheríchia coli
Enzims microbians
Escherichia coli
Microbial enzymes
topic Escheríchia coli
Enzims microbians
Escherichia coli
Microbial enzymes
description A crude cell-free system from an Escherichia coli strain overexpressing the cluster containing the three genes yfgA, yfgB, and gcpE converted 2-C-methyl-d-erythritol 2,4-cyclodiphosphate (1) into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol (6), which most probably represents a novel intermediate in the methylerythritol phosphate pathway for isoprenoid biosynthesis. The free diol 6 was accumulated by phosphatase treatment of the crude enzyme preparation and was identified by comparison with a synthetic reference.
publishDate 2002
dc.date.none.fl_str_mv 2002
2026
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/225926
url https://hdl.handle.net/2445/225926
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/S0040-4039(02)00003-5
Tetrahedron Letters, 2002, vol. 43, num.8, p. 1413-1415
https://doi.org/10.1016/S0040-4039(02)00003-5
dc.rights.none.fl_str_mv (c) Elsevier Ltd., 2002
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Elsevier Ltd., 2002
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 3 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ltd.
publisher.none.fl_str_mv Elsevier Ltd.
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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