Isoprenoid biosynthesis via the methylerythritol phosphate pathway. (E)-4-Hydroxy-3-methylbut-2-enyl diphosphate: chemical synthesis and formation from methylerythritol cyclodiphosphate by a cell-free system from Escherichia coli

2-C-Methyl-d-erythritol cyclodiphosphate is converted into (E)-4-hydroxy-3-methylbut-2-enyl diphosphate by a cell-free system from an Escherichia coli strain overexpressing the gcpE gene. The latter diphosphate, representing probably the last intermediate in the MEP pathway for isoprenoid biosynthes...

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Detalles Bibliográficos
Autores: Wolff, Murielle, Seemann, Myriam, Grosdemange Billiard, Catherine, Tritsch, Denis, Campos Martínez, Narciso, Rodríguez Concepción, Manuel, Boronat i Margosa, Albert, Rohmer, Michel
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2002
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/225957
Acceso en línea:https://hdl.handle.net/2445/225957
Access Level:acceso abierto
Palabra clave:Química bioinorgànica
Escheríchia coli
Bioinorganic chemistry
Escherichia coli
Descripción
Sumario:2-C-Methyl-d-erythritol cyclodiphosphate is converted into (E)-4-hydroxy-3-methylbut-2-enyl diphosphate by a cell-free system from an Escherichia coli strain overexpressing the gcpE gene. The latter diphosphate, representing probably the last intermediate in the MEP pathway for isoprenoid biosynthesis, was identified by comparison with reference material obtained by chemical synthesis.

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