Isotopic Tracers Unveil Distinct Fates for Nitrogen Sources during Wine Fermentation with Two Non-Saccharomyces Strains

© 2020 by the authors.

Detalles Bibliográficos
Autores: Su, Ying, Seguinot, Pauline, Bloem, Audrey, Ortiz-Julien, Anne, Heras, José María, Guillamón, José Manuel, Camarasa, Carole
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/215688
Acceso en línea:http://hdl.handle.net/10261/215688
Access Level:acceso abierto
Palabra clave:15N- and 13C-isotope labelling
Quantitative analysis of metabolism
Nitrogen resource management
Torulaspora delbrueckii
Metschnikowia pulcherrima
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spelling Isotopic Tracers Unveil Distinct Fates for Nitrogen Sources during Wine Fermentation with Two Non-Saccharomyces StrainsSu, YingSeguinot, PaulineBloem, AudreyOrtiz-Julien, AnneHeras, José MaríaGuillamón, José ManuelCamarasa, Carole15N- and 13C-isotope labellingQuantitative analysis of metabolismNitrogen resource managementTorulaspora delbrueckiiMetschnikowia pulcherrima© 2020 by the authors.Non-Saccharomyces yeast strains have become increasingly prevalent in the food industry, particularly in winemaking, because of their properties of interest both in biological control and in complexifying flavour profiles in end-products. However, unleashing the full potential of these species would require solid knowledge of their physiology and metabolism, which is, however, very limited to date. In this study, a quantitative analysis using 15N-labelled NH4Cl, arginine, and glutamine, and 13C-labelled leucine and valine revealed the specificities of the nitrogen metabolism pattern of two non-Saccharomyces species, Torulaspora delbrueckii and Metschnikowia pulcherrima. In T. delbrueckii, consumed nitrogen sources were mainly directed towards the de novo synthesis of proteinogenic amino acids, at the expense of volatile compounds production. This redistribution pattern was in line with the high biomass-producer phenotype of this species. Conversely, in M. pulcherrima, which displayed weaker growth capacities, a larger proportion of consumed amino acids was catabolised for the production of higher alcohols through the Ehrlich pathway. Overall, this comprehensive overview of nitrogen redistribution in T. delbrueckii and M. pulcherrima provides valuable information for a better management of co- or sequential fermentation combining these species with Saccharomyces cerevisiae.Pauline Seguinot was funded by the Lallemand Inc. and Ying Su was supported a fellowship granted by the Generalitat Valencia.Peer reviewedMultidisciplinary Digital Publishing InstituteGeneralitat ValencianaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/215688reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3390/microorganisms8060904Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2156882026-05-22T06:33:51Z
dc.title.none.fl_str_mv Isotopic Tracers Unveil Distinct Fates for Nitrogen Sources during Wine Fermentation with Two Non-Saccharomyces Strains
title Isotopic Tracers Unveil Distinct Fates for Nitrogen Sources during Wine Fermentation with Two Non-Saccharomyces Strains
spellingShingle Isotopic Tracers Unveil Distinct Fates for Nitrogen Sources during Wine Fermentation with Two Non-Saccharomyces Strains
Su, Ying
15N- and 13C-isotope labelling
Quantitative analysis of metabolism
Nitrogen resource management
Torulaspora delbrueckii
Metschnikowia pulcherrima
title_short Isotopic Tracers Unveil Distinct Fates for Nitrogen Sources during Wine Fermentation with Two Non-Saccharomyces Strains
title_full Isotopic Tracers Unveil Distinct Fates for Nitrogen Sources during Wine Fermentation with Two Non-Saccharomyces Strains
title_fullStr Isotopic Tracers Unveil Distinct Fates for Nitrogen Sources during Wine Fermentation with Two Non-Saccharomyces Strains
title_full_unstemmed Isotopic Tracers Unveil Distinct Fates for Nitrogen Sources during Wine Fermentation with Two Non-Saccharomyces Strains
title_sort Isotopic Tracers Unveil Distinct Fates for Nitrogen Sources during Wine Fermentation with Two Non-Saccharomyces Strains
dc.creator.none.fl_str_mv Su, Ying
Seguinot, Pauline
Bloem, Audrey
Ortiz-Julien, Anne
Heras, José María
Guillamón, José Manuel
Camarasa, Carole
author Su, Ying
author_facet Su, Ying
Seguinot, Pauline
Bloem, Audrey
Ortiz-Julien, Anne
Heras, José María
Guillamón, José Manuel
Camarasa, Carole
author_role author
author2 Seguinot, Pauline
Bloem, Audrey
Ortiz-Julien, Anne
Heras, José María
Guillamón, José Manuel
Camarasa, Carole
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Generalitat Valenciana
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv 15N- and 13C-isotope labelling
Quantitative analysis of metabolism
Nitrogen resource management
Torulaspora delbrueckii
Metschnikowia pulcherrima
topic 15N- and 13C-isotope labelling
Quantitative analysis of metabolism
Nitrogen resource management
Torulaspora delbrueckii
Metschnikowia pulcherrima
description © 2020 by the authors.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/215688
url http://hdl.handle.net/10261/215688
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3390/microorganisms8060904
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418868167933952
score 15,81155

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