Expression Profiling Analysis Reveals Key MicroRNA– mRNA Interactions in Early Retinal Degeneration in Retinitis Pigmentosa

PURPOSE. The aim of this study was to identify differentially expressed microRNAs (miRNAs) that might play an important role in the etiology of retinal degeneration in a genetic mouse model of retinitis pigmentosa (rd10 mice) at initial stages of the disease. Methods. miRNAs-mRNA interaction network...

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Autores: Anasagasti, Ander, Ezquerra-Inchausti, Maitane, Barandika, Olatz, Muñoz Culla, Maider, Caffarel, María M., Otaegui Bichot, David, López de Munain Arregui, Adolfo José, Ruiz Ederra, Javier
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/29723
Acceso en línea:http://hdl.handle.net/10810/29723
Access Level:acceso abierto
Palabra clave:retinitis pigmentosa
miRNA
rd10
network analysis
miRNA-mRNA interaction
rod cgmp phosphodiesterase
rd10 mouse
diabetic-retinopathy
target prediction
gene-expression
binding-sites
beta-subunit
apoptosis
cells
protein
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spelling Expression Profiling Analysis Reveals Key MicroRNA– mRNA Interactions in Early Retinal Degeneration in Retinitis PigmentosaAnasagasti, AnderEzquerra-Inchausti, MaitaneBarandika, OlatzMuñoz Culla, MaiderCaffarel, María M.Otaegui Bichot, DavidLópez de Munain Arregui, Adolfo JoséRuiz Ederra, Javierretinitis pigmentosamiRNArd10network analysismiRNA-mRNA interactionrod cgmp phosphodiesteraserd10 mousediabetic-retinopathytarget predictiongene-expressionbinding-sitesbeta-subunitapoptosiscellsproteinPURPOSE. The aim of this study was to identify differentially expressed microRNAs (miRNAs) that might play an important role in the etiology of retinal degeneration in a genetic mouse model of retinitis pigmentosa (rd10 mice) at initial stages of the disease. Methods. miRNAs-mRNA interaction networks were generated for analysis of biological pathways involved in retinal degeneration. RESULTS. Of more than 1900 miRNAs analyzed, we selected 19 miRNAs on the basis of (1) a significant differential expression in rd10 retinas compared with control samples and (2) an inverse expression relationship with predicted mRNA targets involved in biological pathways relevant to retinal biology and/or degeneration. Seven of the selected miRNAs have been associated with retinal dystrophies, whereas, to our knowledge, nine have not been previously linked to any disease. CONCLUSIONS. This study contributes to our understanding of the etiology and progression of retinal degeneration.Supported by the Fundacion Jesus de Gangoiti Barrera and from the Basque Government's Department of Industry and Education Grants SAIOTEK-PE11BN002, PC12BN001, and DEPLC13/002 (AA, JRE); funds from Foundation of Patients of Retinitis Pigmentosa of Gipuzkoa (Retinosis Gipuzkoa Begisare) (OB); a grant from the Fundacion Mutua Madrilena (OB); Basque Government's Department of Education grants DEDUC14/309 (MEI), Diputacion Foral de Gipuzkoa DFG15/006 (MM-C), and ELKARTEK 16/014 (MM-C); National Institute of Health Carlos III (Instituto de Salud Carlos III) Grants ISCIII: CP10/00572 (JRE), PI13/02621 (JRE); an Intensificacion Contract (ALdM) from the Basque Government's Department of Industry; and a grant from the Foundation of Patients of Retinitis Pigmentosa of Gipuzkoa (Retinosis Gipuzkoa Begisare) (JRE). JR-E is a Miguel Servet II Fellow, National Institute of Health Carlos III (Instituto de Salud Carlos III), ISCIII: CPII16/00012.Association Research Vision Ophthalmology201820182018info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/29723reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://iovs.arvojournals.org/article.aspx?articleid=2681208info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/This work is licensed under a Creative Commons Attribution 4.0 International License.Atribución 3.0 Españaoai:addi.ehu.eus:10810/297232026-06-18T09:23:17Z
dc.title.none.fl_str_mv Expression Profiling Analysis Reveals Key MicroRNA– mRNA Interactions in Early Retinal Degeneration in Retinitis Pigmentosa
title Expression Profiling Analysis Reveals Key MicroRNA– mRNA Interactions in Early Retinal Degeneration in Retinitis Pigmentosa
spellingShingle Expression Profiling Analysis Reveals Key MicroRNA– mRNA Interactions in Early Retinal Degeneration in Retinitis Pigmentosa
Anasagasti, Ander
retinitis pigmentosa
miRNA
rd10
network analysis
miRNA-mRNA interaction
rod cgmp phosphodiesterase
rd10 mouse
diabetic-retinopathy
target prediction
gene-expression
binding-sites
beta-subunit
apoptosis
cells
protein
title_short Expression Profiling Analysis Reveals Key MicroRNA– mRNA Interactions in Early Retinal Degeneration in Retinitis Pigmentosa
title_full Expression Profiling Analysis Reveals Key MicroRNA– mRNA Interactions in Early Retinal Degeneration in Retinitis Pigmentosa
title_fullStr Expression Profiling Analysis Reveals Key MicroRNA– mRNA Interactions in Early Retinal Degeneration in Retinitis Pigmentosa
title_full_unstemmed Expression Profiling Analysis Reveals Key MicroRNA– mRNA Interactions in Early Retinal Degeneration in Retinitis Pigmentosa
title_sort Expression Profiling Analysis Reveals Key MicroRNA– mRNA Interactions in Early Retinal Degeneration in Retinitis Pigmentosa
dc.creator.none.fl_str_mv Anasagasti, Ander
Ezquerra-Inchausti, Maitane
Barandika, Olatz
Muñoz Culla, Maider
Caffarel, María M.
Otaegui Bichot, David
López de Munain Arregui, Adolfo José
Ruiz Ederra, Javier
author Anasagasti, Ander
author_facet Anasagasti, Ander
Ezquerra-Inchausti, Maitane
Barandika, Olatz
Muñoz Culla, Maider
Caffarel, María M.
Otaegui Bichot, David
López de Munain Arregui, Adolfo José
Ruiz Ederra, Javier
author_role author
author2 Ezquerra-Inchausti, Maitane
Barandika, Olatz
Muñoz Culla, Maider
Caffarel, María M.
Otaegui Bichot, David
López de Munain Arregui, Adolfo José
Ruiz Ederra, Javier
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv retinitis pigmentosa
miRNA
rd10
network analysis
miRNA-mRNA interaction
rod cgmp phosphodiesterase
rd10 mouse
diabetic-retinopathy
target prediction
gene-expression
binding-sites
beta-subunit
apoptosis
cells
protein
topic retinitis pigmentosa
miRNA
rd10
network analysis
miRNA-mRNA interaction
rod cgmp phosphodiesterase
rd10 mouse
diabetic-retinopathy
target prediction
gene-expression
binding-sites
beta-subunit
apoptosis
cells
protein
description PURPOSE. The aim of this study was to identify differentially expressed microRNAs (miRNAs) that might play an important role in the etiology of retinal degeneration in a genetic mouse model of retinitis pigmentosa (rd10 mice) at initial stages of the disease. Methods. miRNAs-mRNA interaction networks were generated for analysis of biological pathways involved in retinal degeneration. RESULTS. Of more than 1900 miRNAs analyzed, we selected 19 miRNAs on the basis of (1) a significant differential expression in rd10 retinas compared with control samples and (2) an inverse expression relationship with predicted mRNA targets involved in biological pathways relevant to retinal biology and/or degeneration. Seven of the selected miRNAs have been associated with retinal dystrophies, whereas, to our knowledge, nine have not been previously linked to any disease. CONCLUSIONS. This study contributes to our understanding of the etiology and progression of retinal degeneration.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/29723
url http://hdl.handle.net/10810/29723
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://iovs.arvojournals.org/article.aspx?articleid=2681208
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
This work is licensed under a Creative Commons Attribution 4.0 International License.
Atribución 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
This work is licensed under a Creative Commons Attribution 4.0 International License.
Atribución 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Association Research Vision Ophthalmology
publisher.none.fl_str_mv Association Research Vision Ophthalmology
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418704434888704
score 15,300719

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