Expression Profiling Analysis Reveals Key MicroRNA– mRNA Interactions in Early Retinal Degeneration in Retinitis Pigmentosa

PURPOSE. The aim of this study was to identify differentially expressed microRNAs (miRNAs) that might play an important role in the etiology of retinal degeneration in a genetic mouse model of retinitis pigmentosa (rd10 mice) at initial stages of the disease. Methods. miRNAs-mRNA interaction network...

Descripción completa

Detalles Bibliográficos
Autores: Anasagasti, Ander, Ezquerra-Inchausti, Maitane, Barandika, Olatz, Muñoz Culla, Maider, Caffarel, María M., Otaegui Bichot, David, López de Munain Arregui, Adolfo José, Ruiz Ederra, Javier
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/29723
Acceso en línea:http://hdl.handle.net/10810/29723
Access Level:acceso abierto
Palabra clave:retinitis pigmentosa
miRNA
rd10
network analysis
miRNA-mRNA interaction
rod cgmp phosphodiesterase
rd10 mouse
diabetic-retinopathy
target prediction
gene-expression
binding-sites
beta-subunit
apoptosis
cells
protein
Descripción
Sumario:PURPOSE. The aim of this study was to identify differentially expressed microRNAs (miRNAs) that might play an important role in the etiology of retinal degeneration in a genetic mouse model of retinitis pigmentosa (rd10 mice) at initial stages of the disease. Methods. miRNAs-mRNA interaction networks were generated for analysis of biological pathways involved in retinal degeneration. RESULTS. Of more than 1900 miRNAs analyzed, we selected 19 miRNAs on the basis of (1) a significant differential expression in rd10 retinas compared with control samples and (2) an inverse expression relationship with predicted mRNA targets involved in biological pathways relevant to retinal biology and/or degeneration. Seven of the selected miRNAs have been associated with retinal dystrophies, whereas, to our knowledge, nine have not been previously linked to any disease. CONCLUSIONS. This study contributes to our understanding of the etiology and progression of retinal degeneration.

Similares en LA Referencia