Micropropagación del aliso común para la conservación de su germoplasma
[EN] Shoot cultures from adult material of common alder (Alnus glutinosa) were established in vitro. Branches from the canopy and stump sprouts were collected from 20-30-year-old trees. Shoots developed from these branches were used to initiate the cultures using Woody Plant Medium supplemented with...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2010 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/39682 |
| Acceso en línea: | http://hdl.handle.net/10261/39682 |
| Access Level: | acceso abierto |
| Palabra clave: | Micropropagación Aliso Alnus Conservación |
| Sumario: | [EN] Shoot cultures from adult material of common alder (Alnus glutinosa) were established in vitro. Branches from the canopy and stump sprouts were collected from 20-30-year-old trees. Shoots developed from these branches were used to initiate the cultures using Woody Plant Medium supplemented with 2 mg/l benzyadenine and 0.5 mg/l indole acetic acid. The multiplication stage was divided into 3 cycles of 3 weeks each, transferring the shoots to fresh medium at the end of each cycle. Woody Plant Medium was used in this stage supplemented with 0.5 mg/l indole acetic acid, 0.2 mg/l benzyladenine (during the first cycle, 0.1 mg/l for the next two), and 0.5 mg/l zeatin in the 3rd cycle. Shoots (1.5-2 cm) from the multiplication stage were rooted in vitro. Rooting percentages getting up to 75% both in controls and with 0.1 mg/l indole butyric acid, although the presence of auxin increased the speed of root formation. |
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