Morphology of bile salts micelles and mixed micelles with lipolysis products, from scattering techniques and atomistic simulations

[EN]Hypotheses: Bile salts (BS) are biosurfactants released into the small intestine, which play key and contrasting roles in lipid digestion: they adsorb at interfaces and promote the adsorption of digestive enzymes onto fat droplets, while they also remove lipolysis products from that interface, s...

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Bibliographic Details
Authors: Pabois, Olivia, Ziolek, Robert M., Lorenz, Christian D., Prévost, Sylvain, Mahmoudi, Najet, Skoda, Maximilian W.A., Welbourn, Rebecca J.L., Valero Juan, Margarita, Harvey, Richard D., Grundy, Myriam M. L., Wilde, Peter J., Grillo, Isabelle, Gerelli, Yuri, Dreiss, Cécile A.
Format: article
Status:Published version
Publication Date:2020
Country:España
Institution:Universidad de Salamanca (USAL)
Repository:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:dnet:gredos______::a0c6392fb624c71e4a82876b344b1d86
Online Access:http://hdl.handle.net/10366/170993
Access Level:Open access
Keyword:Bile salts
Bulk aggregation properties
Lipid digestion
Lipolysis products
Liposomes
Small-angle scattering
Lipolysis
Micelles
liposomas
micelas
lipólisis
Description
Summary:[EN]Hypotheses: Bile salts (BS) are biosurfactants released into the small intestine, which play key and contrasting roles in lipid digestion: they adsorb at interfaces and promote the adsorption of digestive enzymes onto fat droplets, while they also remove lipolysis products from that interface, solubilising them into mixed micelles. Small architectural variations on their chemical structure, specifically their bile acid moiety, are hypothesised to underlie these conflicting functionalities, which should be reflected in different aggregation and solubilisation behaviour. Experiments: The micellisation of two BS, sodium taurocholate (NaTC) and sodium taurodeoxycholate (NaTDC), which differ by one hydroxyl group on the bile acid moiety, was assessed by pyrene fluorescence spectroscopy, and the morphology of aggregates formed in the absence and presence of fatty acids (FA) and monoacylglycerols (MAG) – typical lipolysis products – was resolved by small-angle X-ray/neutron scattering (SAXS, SANS) and molecular dynamics simulations. The solubilisation by BS of triacylglycerolincorporating liposomes – mimicking ingested lipids – was studied by neutron reflectometry and SANS.

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