Morphology of bile salts micelles and mixed micelles with lipolysis products, from scattering techniques and atomistic simulations

[EN]Hypotheses: Bile salts (BS) are biosurfactants released into the small intestine, which play key and contrasting roles in lipid digestion: they adsorb at interfaces and promote the adsorption of digestive enzymes onto fat droplets, while they also remove lipolysis products from that interface, s...

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Detalles Bibliográficos
Autores: Pabois, Olivia, Ziolek, Robert M., Lorenz, Christian D., Prévost, Sylvain, Mahmoudi, Najet, Skoda, Maximilian W.A., Welbourn, Rebecca J.L., Valero Juan, Margarita, Harvey, Richard D., Grundy, Myriam M. L., Wilde, Peter J., Grillo, Isabelle, Gerelli, Yuri, Dreiss, Cécile A.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:dnet:gredos______::a0c6392fb624c71e4a82876b344b1d86
Acceso en línea:http://hdl.handle.net/10366/170993
Access Level:acceso abierto
Palabra clave:Bile salts
Bulk aggregation properties
Lipid digestion
Lipolysis products
Liposomes
Small-angle scattering
Lipolysis
Micelles
liposomas
micelas
lipólisis
Descripción
Sumario:[EN]Hypotheses: Bile salts (BS) are biosurfactants released into the small intestine, which play key and contrasting roles in lipid digestion: they adsorb at interfaces and promote the adsorption of digestive enzymes onto fat droplets, while they also remove lipolysis products from that interface, solubilising them into mixed micelles. Small architectural variations on their chemical structure, specifically their bile acid moiety, are hypothesised to underlie these conflicting functionalities, which should be reflected in different aggregation and solubilisation behaviour. Experiments: The micellisation of two BS, sodium taurocholate (NaTC) and sodium taurodeoxycholate (NaTDC), which differ by one hydroxyl group on the bile acid moiety, was assessed by pyrene fluorescence spectroscopy, and the morphology of aggregates formed in the absence and presence of fatty acids (FA) and monoacylglycerols (MAG) – typical lipolysis products – was resolved by small-angle X-ray/neutron scattering (SAXS, SANS) and molecular dynamics simulations. The solubilisation by BS of triacylglycerolincorporating liposomes – mimicking ingested lipids – was studied by neutron reflectometry and SANS.

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