Changes in white adipose tissue metabolism induced by resveratrol in rats.

BACKGROUND: A remarkable range of biological functions have been ascribed to resveratrol. Recently, this polyphenol has been shown to have body fat lowering effects. The aim of the present study was to assess some of the potential underlying mechanisms of action which take place in adipose tissue. M...

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Autores: Alberdi, Goiuri, Rodríguez, Víctor Manuel, Miranda, Jonatan, Mascarulla, María T., Arias, Noemí, Andrés Lacueva, Ma. Cristina, Portillo, María Puy
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/68564
Acceso en línea:https://hdl.handle.net/2445/68564
Access Level:acceso abierto
Palabra clave:Polifenols
Teixit adipós
Polyphenols
Adipose tissues
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spelling Changes in white adipose tissue metabolism induced by resveratrol in rats.Alberdi, GoiuriRodríguez, Víctor ManuelMiranda, JonatanMascarulla, María T.Arias, NoemíAndrés Lacueva, Ma. CristinaPortillo, María PuyPolifenolsTeixit adipósPolyphenolsAdipose tissuesBACKGROUND: A remarkable range of biological functions have been ascribed to resveratrol. Recently, this polyphenol has been shown to have body fat lowering effects. The aim of the present study was to assess some of the potential underlying mechanisms of action which take place in adipose tissue. METHODS: Sixteen male Sprague-Dawley rats were randomly divided into two groups: control and treated with 30 mg resveratrol/kg body weight/d. All rats were fed an obesogenic diet and after six weeks of treatment white adipose tissues were dissected. Lipoprotein lipase activity was assessed by fluorimetry, acetyl-CoA carboxylase by radiometry, and malic enzyme, glucose-6P-dehydrogenase and fatty acid synthase by spectrophotometry. Gene expression levels of acetyl-CoA carboxylase, fatty acid synthase, lipoprotein lipase, hormone-sensitive lipase, adipose triglyceride lipase, PPAR-gamma, SREBP-1c and perilipin were assessed by Real time RT-PCR. The amount of resveratrol metabolites in adipose tissue was measured by chromatography. RESULTS: There was no difference in the final body weight of the rats; however, adipose tissues were significantly decreased in the resveratrol-treated group. Resveratrol reduced the activity of lipogenic enzymes, as well as that of heparin-releasable lipoprotein lipase. Moreover, a significant reduction was induced by this polyphenol in hormone-sensitive lipase mRNA levels. No significant changes were observed in other genes. Total amount of resveratrol metabolites in adipose tissue was 2.66 ± 0.55 nmol/g tissue. CONCLUSIONS: It can be proposed that the body fat-lowering effect of resveratrol is mediated, at least in part, by a reduction in fatty acid uptake from circulating triacylglycerols and also in de novo lipogenesis.BioMed Central2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/68564Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1186/1743-7075-8-29Nutrition and Metabolism, 2011, vol. 8, p. 29http://dx.doi.org/10.1186/1743-7075-8-29cc-by (c) Alberdi, Goiuri et al., 2011http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/685642026-05-27T06:46:51Z
dc.title.none.fl_str_mv Changes in white adipose tissue metabolism induced by resveratrol in rats.
title Changes in white adipose tissue metabolism induced by resveratrol in rats.
spellingShingle Changes in white adipose tissue metabolism induced by resveratrol in rats.
Alberdi, Goiuri
Polifenols
Teixit adipós
Polyphenols
Adipose tissues
title_short Changes in white adipose tissue metabolism induced by resveratrol in rats.
title_full Changes in white adipose tissue metabolism induced by resveratrol in rats.
title_fullStr Changes in white adipose tissue metabolism induced by resveratrol in rats.
title_full_unstemmed Changes in white adipose tissue metabolism induced by resveratrol in rats.
title_sort Changes in white adipose tissue metabolism induced by resveratrol in rats.
dc.creator.none.fl_str_mv Alberdi, Goiuri
Rodríguez, Víctor Manuel
Miranda, Jonatan
Mascarulla, María T.
Arias, Noemí
Andrés Lacueva, Ma. Cristina
Portillo, María Puy
author Alberdi, Goiuri
author_facet Alberdi, Goiuri
Rodríguez, Víctor Manuel
Miranda, Jonatan
Mascarulla, María T.
Arias, Noemí
Andrés Lacueva, Ma. Cristina
Portillo, María Puy
author_role author
author2 Rodríguez, Víctor Manuel
Miranda, Jonatan
Mascarulla, María T.
Arias, Noemí
Andrés Lacueva, Ma. Cristina
Portillo, María Puy
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Polifenols
Teixit adipós
Polyphenols
Adipose tissues
topic Polifenols
Teixit adipós
Polyphenols
Adipose tissues
description BACKGROUND: A remarkable range of biological functions have been ascribed to resveratrol. Recently, this polyphenol has been shown to have body fat lowering effects. The aim of the present study was to assess some of the potential underlying mechanisms of action which take place in adipose tissue. METHODS: Sixteen male Sprague-Dawley rats were randomly divided into two groups: control and treated with 30 mg resveratrol/kg body weight/d. All rats were fed an obesogenic diet and after six weeks of treatment white adipose tissues were dissected. Lipoprotein lipase activity was assessed by fluorimetry, acetyl-CoA carboxylase by radiometry, and malic enzyme, glucose-6P-dehydrogenase and fatty acid synthase by spectrophotometry. Gene expression levels of acetyl-CoA carboxylase, fatty acid synthase, lipoprotein lipase, hormone-sensitive lipase, adipose triglyceride lipase, PPAR-gamma, SREBP-1c and perilipin were assessed by Real time RT-PCR. The amount of resveratrol metabolites in adipose tissue was measured by chromatography. RESULTS: There was no difference in the final body weight of the rats; however, adipose tissues were significantly decreased in the resveratrol-treated group. Resveratrol reduced the activity of lipogenic enzymes, as well as that of heparin-releasable lipoprotein lipase. Moreover, a significant reduction was induced by this polyphenol in hormone-sensitive lipase mRNA levels. No significant changes were observed in other genes. Total amount of resveratrol metabolites in adipose tissue was 2.66 ± 0.55 nmol/g tissue. CONCLUSIONS: It can be proposed that the body fat-lowering effect of resveratrol is mediated, at least in part, by a reduction in fatty acid uptake from circulating triacylglycerols and also in de novo lipogenesis.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/68564
url https://hdl.handle.net/2445/68564
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1186/1743-7075-8-29
Nutrition and Metabolism, 2011, vol. 8, p. 29
http://dx.doi.org/10.1186/1743-7075-8-29
dc.rights.none.fl_str_mv cc-by (c) Alberdi, Goiuri et al., 2011
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Alberdi, Goiuri et al., 2011
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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