Fission yeast mfr1 activates APC and coordinates meiotic nuclear division with sporulation

Meiosis is the developmental program by which sexually reproducing diploid organisms generate haploid gametes. In yeast, meiosis is followed by spore morphogenesis. These two events are normally coordinated in such a way that spore formation is dependent upon completion of the meiotic nuclear divisi...

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Detalles Bibliográficos
Autores: Blanco Rodríguez, Miguel A., Pelloquin, Laetitia, Moreno, Sergio
Tipo de recurso: artículo
Fecha de publicación:2001
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/66442
Acceso en línea:http://hdl.handle.net/10261/66442
Access Level:acceso abierto
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spelling Fission yeast mfr1 activates APC and coordinates meiotic nuclear division with sporulationBlanco Rodríguez, Miguel A.Pelloquin, LaetitiaMoreno, SergioMeiosis is the developmental program by which sexually reproducing diploid organisms generate haploid gametes. In yeast, meiosis is followed by spore morphogenesis. These two events are normally coordinated in such a way that spore formation is dependent upon completion of the meiotic nuclear divisions. Here we describe a meiosis-specific protein, mfr1, that is involved in this coordination. mfr1 is an activator of the anaphase-promoting complex (APC), which is necessary for the rapid degradation of the cdc13 cyclin at the end of meiosis II, prior to the formation of spores. An mfr1 null mutant completes meiosis II but remains with high levels of cdc13 and cdc2 kinase activity and has considerably delayed spore formation. By analogy with the mitotic cell cycle, where proteolysis and inactivation of cdc2 kinase are necessary to trigger mitotic exit and cytokinesis, we propose that at the end of meiosis rapid and timely proteolysis of cyclins is required to switch on the differentiation program that eventually leads to the formation of haploid gametes.This work was supported by funding from the Comision Interministerial de Ciencia y Tecnología (CICYT) and the European Union.Peer ReviewedCompany of Biologists2013201320012013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/66442reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://jcs.biologists.org/content/114/11/2135.longinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/664422026-05-22T06:33:51Z
dc.title.none.fl_str_mv Fission yeast mfr1 activates APC and coordinates meiotic nuclear division with sporulation
title Fission yeast mfr1 activates APC and coordinates meiotic nuclear division with sporulation
spellingShingle Fission yeast mfr1 activates APC and coordinates meiotic nuclear division with sporulation
Blanco Rodríguez, Miguel A.
title_short Fission yeast mfr1 activates APC and coordinates meiotic nuclear division with sporulation
title_full Fission yeast mfr1 activates APC and coordinates meiotic nuclear division with sporulation
title_fullStr Fission yeast mfr1 activates APC and coordinates meiotic nuclear division with sporulation
title_full_unstemmed Fission yeast mfr1 activates APC and coordinates meiotic nuclear division with sporulation
title_sort Fission yeast mfr1 activates APC and coordinates meiotic nuclear division with sporulation
dc.creator.none.fl_str_mv Blanco Rodríguez, Miguel A.
Pelloquin, Laetitia
Moreno, Sergio
author Blanco Rodríguez, Miguel A.
author_facet Blanco Rodríguez, Miguel A.
Pelloquin, Laetitia
Moreno, Sergio
author_role author
author2 Pelloquin, Laetitia
Moreno, Sergio
author2_role author
author
description Meiosis is the developmental program by which sexually reproducing diploid organisms generate haploid gametes. In yeast, meiosis is followed by spore morphogenesis. These two events are normally coordinated in such a way that spore formation is dependent upon completion of the meiotic nuclear divisions. Here we describe a meiosis-specific protein, mfr1, that is involved in this coordination. mfr1 is an activator of the anaphase-promoting complex (APC), which is necessary for the rapid degradation of the cdc13 cyclin at the end of meiosis II, prior to the formation of spores. An mfr1 null mutant completes meiosis II but remains with high levels of cdc13 and cdc2 kinase activity and has considerably delayed spore formation. By analogy with the mitotic cell cycle, where proteolysis and inactivation of cdc2 kinase are necessary to trigger mitotic exit and cytokinesis, we propose that at the end of meiosis rapid and timely proteolysis of cyclins is required to switch on the differentiation program that eventually leads to the formation of haploid gametes.
publishDate 2001
dc.date.none.fl_str_mv 2001
2013
2013
2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/66442
url http://hdl.handle.net/10261/66442
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://jcs.biologists.org/content/114/11/2135.long
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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