3D chromatin remodelling in the germ line modulates genome evolutionary plasticity

Chromosome folding has profound impacts on gene regulation, whose evolutionary consequences are far from being understood. Here we explore the relationship between 3D chromatin remodelling in mouse germ cells and evolutionary changes in genome structure. Using a comprehensive integrative computation...

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Detalles Bibliográficos
Autores: Álvarez-González, Lucía|||0000-0001-8154-8614, Burden, Frances, Doddamani, Dadakhalandar, Malinverni, Roberto|||0000-0002-0113-3417, Leach, Emma|||0000-0002-9781-4021, Marin Garcia, Cristina|||0000-0002-5008-1078, Marin Gual, Laia|||0000-0003-1480-0976, Gubern Burset, Albert|||0000-0001-6458-1498, Vara González, Covadonga|||0000-0002-8264-9262, Paytuví Gallart, Andreu|||0000-0002-0958-6592, Buschbeck, Marcus|||0000-0002-3218-4567, Ellis, Peter J. I.|||0000-0001-9709-7934, Farré, Marta|||0000-0001-9170-5767, Ruiz Herrera Moreno, Aurora|||0000-0003-3868-6151
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:263136
Acceso en línea:https://ddd.uab.cat/record/263136
https://dx.doi.org/urn:doi:10.1038/s41467-022-30296-6
Access Level:acceso abierto
Palabra clave:Animals
Chromatin
Chromatin Assembly and Disassembly
DNA Breaks, Double-Stranded
Genome
Germ Cells
Male
Meiosis
Mice
Spermatogenesis
Descripción
Sumario:Chromosome folding has profound impacts on gene regulation, whose evolutionary consequences are far from being understood. Here we explore the relationship between 3D chromatin remodelling in mouse germ cells and evolutionary changes in genome structure. Using a comprehensive integrative computational analysis, we (i) reconstruct seven ancestral rodent genomes analysing whole-genome sequences of 14 species representatives of the major phylogroups, (ii) detect lineage-specific chromosome rearrangements and (iii) identify the dynamics of the structural and epigenetic properties of evolutionary breakpoint regions (EBRs) throughout mouse spermatogenesis. Our results show that EBRs are devoid of programmed meiotic DNA double-strand breaks (DSBs) and meiotic cohesins in primary spermatocytes, but are associated in post-meiotic cells with sites of DNA damage and functional long-range interaction regions that recapitulate ancestral chromosomal configurations. Overall, we propose a model that integrates evolutionary genome reshuffling with DNA damage response mechanisms and the dynamic spatial genome organisation of germ cells.

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